Morphological and molecular characterization, sexual reproduction, and pathogenicity of<i>Setosphaeria rostrata</i>isolates from rice leaf spot

Kusai, Nor Azizah; Azmi, Madihah Mior Zakuan; Zainudin, Nur Ain Izzati Mohd; Yusof, Mohd Termizi; Razak, Azmi Abd

doi:10.3852/15-175

Cited by 13 publications

(7 citation statements)

References 46 publications

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“…In contrast to B. oryzae populations, E. rostratum populations showed a dominant distribution of a single mating type, MAT1-2 (79%). These results are similar to those of Kusai et al (2016), observed on E. rostratum isolates from rice in Malaysia. The linkage disequilibrium and homoplasy tests did not reject the hypothesis of recombination within the E. rostratum populations of Burkina Faso.…”

Section: Rodolfisupporting

confidence: 91%

“…Identification of mating types was done by amplification of MAT1-1 and MAT1-2 genes with primers specific to B. oryzae (Ahmadpour et al, 2018) and E. rostratum (Kusai et al, 2016). Each PCR reaction using the kit Go-Taq (Promega) had a final volume of 25 mL, containing 1 mL of template genomic DNA (20 ng/µL),1 mL forward primer (10 µM), 1 mL reverse primer (10 µM), 1µL of dNTPs (10 µM each), 5 mL of the buffer 5x, 0.25 µL Taq polymerase (5U/µL) and 14.75 mL sterile water.…”

Section: Mating Type Characterization and In Vitro Sexual Reproductionmentioning

confidence: 99%

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Genetic diversity and structure of Bipolaris oryzae and Exserohilum rostratum populations causing brown spot of rice in Burkina Faso based on genotyping-by-sequencing

et al. 2022

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In recent years, Brown spot disease of rice (BSR) has been observed on leaves and seeds of rice in all rice-growing areas of Burkina Faso. Bipolaris oryzae and Exserohilum rostratum are the main fungal species isolated from BSR infected tissues and they are frequently observed in the same field. However, we are lacking information on the genetic diversity and population structure of these fungi in Burkina Faso. The mode of reproduction is also unknown. The genetic diversity of isolates of B. oryzae (n=61) and E. rostratum (n=151), collected from major rice-growing areas of Burkina Faso, was estimated using genotyping-by-sequencing (GBS). The mean values for nucleotide diversity (π) were 1.9 x10-4 for B. oryzae and 4.8 x10-4 for E. rostratum. There is no genetic differentiation between the geographical populations of each species. The analysis of molecular variance revealed that 89% and 94% of the genetic variances were within the populations of B. oryzae and E. rostratum, respectively. For each species, four genetic clusters were identified by two clustering methods (DAPC and sNMF). The distribution of these genetic groups was independent of the geographical origin of the isolates. Evidence of recombination was detected in the populations of B. oryzae and E. rostratum. For B. oryzae balanced mating type ratios were supporting sexual reproduction. For E. rostratum overrepresentation of MAT1-2 isolates (79%) suggested a predominant asexual reproduction. This study provides important information on the biology and genetics of the two major fungi causing brown spot disease of rice in Burkina Faso.

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Section: Rodolfisupporting

confidence: 91%

Section: Mating Type Characterization and In Vitro Sexual Reproductionmentioning

confidence: 99%

Genetic diversity and structure of Bipolaris oryzae and Exserohilum rostratum populations causing brown spot of rice in Burkina Faso based on genotyping-by-sequencing

et al. 2022

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“…It was reported that Phoma sp causes leaf spot disease on Schisandra chinensis in Korea (Choi, 2014). Setosphaeria rostrata is a common plant pathogen causing leaf spot disease, affects a wide range of plant species, mainly grasses (Kusai et al, 2016).…”

Section: Resultsmentioning

confidence: 99%

Molecular characterization of the main fungi associated to Bambara groundnut foliar diseases in Burkina Faso

Ouoba¹,

Zida²,

Soalla³

et al. 2019

J. App. Bioscience.

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Objective: This study aims to update the database of fungi associated to Bambara groundnut foliar diseases in Burkina Faso using both molecular and morphological identification approaches. Methodology and Results: In this study, molecular approach based on the sequencing of ITS (Internal Transcripted Spacer) region of fungi and morphological approach were used to identify the main fungi associated to Bambara groundnut foliar diseases. The study was performed with universal polymerase chain reaction (PCR) primer ITS1/ITS4. BlastN comparisons between 19 fungal isolates contigs of the 16 major fungi were produced by their DNA sequences assembly and GenBank sequences yielded identity scores of 99 to 100 % with all of them. The degrees of similarity between these contigs and the loci sequences of classified fungi in GenBank indicate that our fungal isolates are the same species with those in Genbank, particularly the first of the list show after the blastN. It is the first report of molecular characterization of the main fungi infecting Bambara groundnut in Burkina Faso. Conclusion and Application of results: Nineteen fungi associated to Bambara groundnut foliar diseases were identified and can be taken as targets in varietal improvement of Bambara groundnut for resistance to fungal diseases in Burkina Faso.

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“…Exserohilum has been described as a pathogen on cereals and grasses (Kusai et al 2016). Every year about 10-20% losses due to diseases are reported in wheat (Figueroa et al 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Exserohilum pathogenic species include E. pedicellatum, E. prolatum producing leaf spot on maize and wheat, but E. rostratum infect numerious hosts, including wheat, banana, maize and grasses (Lin et al 2011). Exserohilum leaf blight is characterized by leaf and stem lesions that spread to the entire leaf after a few days of infection (Kusai et al 2016). A strong interaction between the pathogen and the environment is also reported (Kusai et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Pathogenicity and infection behaviour of Exserohilum rostratum on wheat and associated collateral hosts

et al. 2023

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Exserohilum leaf spot is a newly arising fungal disease that mostly affects monocots. Thirty-two plant species of 14 families were evaluated for pathogenicity to Exserohilum rostratum as a potential pathogen. The isolate collected from diseased wheat leaves produced typical dark brown lesions upon inoculation to healthy wheat plants and produced similar symptoms. The arti cial inoculation of detached leaf assay symptoms appeared on major cereals like Triticum aestivum, Oryza sativa, Echinochloa esculenta, Panicum miliaceum and Eleusine coracana. The symptoms were reddish-brown in most of the genera of Poaceae. Histopathological studies revealed that conidia produce the appressoria within 24 hrs and penetrate the host through stomata or epidermal cells after germination. Study reveals that collateral hosts serve as reservoirs for the infection, allowing it to persist in the absence of its primary host. These secondary hosts aid the pathogen in continuing the infection cycle and spreading the disease.

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Morphological and molecular characterization, sexual reproduction, and pathogenicity ofSetosphaeria rostrataisolates from rice leaf spot

Cited by 13 publications

References 46 publications

Genetic diversity and structure of Bipolaris oryzae and Exserohilum rostratum populations causing brown spot of rice in Burkina Faso based on genotyping-by-sequencing

Genetic diversity and structure of Bipolaris oryzae and Exserohilum rostratum populations causing brown spot of rice in Burkina Faso based on genotyping-by-sequencing

Molecular characterization of the main fungi associated to Bambara groundnut foliar diseases in Burkina Faso

Pathogenicity and infection behaviour of Exserohilum rostratum on wheat and associated collateral hosts

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