Morphological and molecular variability among Indian isolates of Rhizoctonia solani causing banded leaf and sheath blight in maize

Singh, V. P.; Amaradasa, Bimal S.; Karjagi, Chikappa G.; Lakshman, Dilip K.; Hooda, K. S.; Kumar, A. S. H.

doi:10.1007/s10658-018-1447-2

Cited by 16 publications

(21 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The isolate RS4 was found to be highly virulent with 78% disease incidence, while RS1 showed only 9.21% disease incidence. Many workers have reported morphological as well as pathological variations in fungal isolates associated with sheath diseases of rice (Guleria et al, 2007;Singh et al, 2015;Ramos-Molina and Chavarro-Mesa, 2016;Singh et al, 2018). Macro-sized sclerotia forming isolate RS4 was observed to be more virulent than isolate RS3 which formed micro-sized sclerotia; and nonsclerotia forming isolate RS1 was the least virulent.…”

Section: Test For Pathogenicity Of Different Isolatesmentioning

confidence: 99%

“…Several studies on rice sheath blight have used molecular markers such as RAPD (Guleria et al, 2007;Susheela, 2012;Lal et al, 2014;Singh et al, 2015), RFLP (Linde et al, 2005), AFLP (Taheri et al, 2007) and ISSR (Guleria et al, 2007;Yugander et al, 2015;Goswami et al, 2017) along with morphological markers. Recently, rDNA-ITS sequencing has been used for identifying variations (Ramos-Molina and Chavarro-Mesa, 2016;Bintang et al, 2017;Singh et al, 2018).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Morphological and Molecular Characterization of Rhizoctonia solani causing Sheath Blight in Rice

Pralhad¹,

Krishnaraj²,

Prashanthi³

2019

Int.J.Curr.Microbiol.App.Sci

View full text Add to dashboard Cite

Section: Test For Pathogenicity Of Different Isolatesmentioning

confidence: 99%

mentioning

confidence: 99%

Morphological and Molecular Characterization of Rhizoctonia solani causing Sheath Blight in Rice

Pralhad¹,

Krishnaraj²,

Prashanthi³

2019

Int.J.Curr.Microbiol.App.Sci

View full text Add to dashboard Cite

“…The infected leaf sheath (5 mm 2 ) and sclerotia of the fungus from each infected sample were clean in sterilize distilled water and surface sterilized in 0.5% sodium hypochlorite solution for 30-60s, washed three times with sterilized distilled water, dried on filter paper and placed on potato dextrose agar (PDA) medium (OXOID, made in the United Kingdom) The plate was at 27-30 o C for 2-3 days incubated (Lal et al 2014;Singh et al 2018). Pure cultures were obtained by re-culture from hyphal tips and maintained on PDA (Priyatmojo et al 2001;Bintang et al 2017).…”

Section: Isolation Of R Solanimentioning

confidence: 99%

“…The mean colony growth was then calculated from the three replicates of each treatment. Based on the colony growth, each isolate was classified into three groups, i.e., fast (48 h), medium (72 h), and slow (96 h or more) growing (Debbarma and Dutta 2015;Singh et al 2018).…”

Section: Culture Morphology Characteristicsmentioning

confidence: 99%

Effect of photoperiod on the cultural morphology of Rhizoctonia solani isolates of maize from Yogyakarta and Central Java, Indonesia

Budiarti

Lukman²,

Sumardiyono

et al. 2019

Biodiversitas

View full text Add to dashboard Cite

Abstract. Budiarti SW, Lukman R, Sumardiyono C, Wibowo A, Priyatmojo A. 2019. Effect of photoperiod on the cultural morphology of Rhizoctonia solani isolates of maize from Yogyakarta and Central Java, Indonesia. Biodiversitas 20: 2028-2038. Banded leaf and sheath blight (BLSB) caused by Rhizoctonia solani Kuhn is a significant constraint of maize production in Indonesia, especially within the last several years. R. solani is an important soil-borne necrotrophic fungal pathogen which is known to be sensitive to different environmental conditions. The differential response of fungi to light and dark is important to study, especially of those causing disease to crop plant as it helps to reveal the factors regulating the pathogenicity of the fungus. This study aims to determine the effect of light duration on the mycelial development and sclerotia formation of R. solani isolates obtained from different locations in Yogyakarta Special Region and Central Java, Indonesia. Ten isolates of R. solani collected from different geographic regions in Yogyakarta Special Region, and Central Java from maize (nine isolates) and rice (one isolate) were exposed to three different light conditions: 12 h alternating light/dark, continuous darkness, and continuous light. Cultural morphology and sclerotial characteristics were recorded for each isolate. All isolates revealed great diversity in terms of cultural and morphological characteristics after exposure to three different light conditions. Under complete darkness, the mycelial growth phase was extended, and a fluffy mass of mycelia was produced. All isolates showed abundant growth under the 12 h alternating light/dark condition than under the continuous dark and continuous light conditions. Sclerotia were initiated earlier under the 12 h alternating light/dark condition than under other conditions. The 12 h alternating light/dark condition also induced number and heavier weight of sclerotia compared with those produced under the continuous light and continuous dark conditions. The modifications in light condition exert a significant impact on some R. solani isolates.

show abstract

“…However, due to improper treatment, straw mulching has provided habitat for many soil-borne pathogens. As an important cereal crop in the global agricultural economy (Feng et al ., 2020), maize ( Zea mays L.) is critical for increased grain yield, but the incidence of banded leaf sheath blight in maize has been increasing annually, resulting in a decline in the quality and yield of maize (Singh et al ., 2018). Currently, farmers have a weak sense of prevention and control of banded leaf sheath blight in maize, and there is little use of control agents.…”

Section: Introductionmentioning

confidence: 99%

Establishment of baseline sensitivity ofRhizoctonia solanito thifluzamide in maize and its field application

et al. 2020

View full text Add to dashboard Cite

In recent years, banded leaf sheath blight in maize (Zea mays L.) has become an important disease that seriously affects quality and yield. This paper aims to evaluate the sensitivity of Rhizoctonia solani Kuhn to thifluzamide on maize, to clarify the effect of seed coating using a thifluzamide suspension agent on safety and physiological indicators and to determine the effectiveness of control of banded leaf sheath blight in the field. In this study, the thifluzamide sensitivity of 102 strains of R. solani in maize from Shandong was determined using the mycelial growth rate method; the average half-maximal effective concentration value (EC50) was 0.086 ± 0.004 μg/ml and displayed a unimodal frequency distribution, indicating that thifluzamide had strong inhibitory activity on the mycelial growth of R. solani in maize. In an indoor pot test, the root activities under 24 g a.i./100 kg seed were found to increase by 78.01%, compared with the control. Similarly, chlorophyll content increased most significantly at this dose, by 32.3%. Thifluzamide (FS) could significantly increase the per-plot yield. Among the examined dosages, 48 g a.i./100 kg seed had the most significant treatment effect, with the yield rate increasing by 15.7% and 14.1%, respectively, in 2017 and 2018 compared with the control. The field effectiveness against banded leaf sheath blight in maize was highest at the dosage of 48 g a.i./100 kg seed for a seed dressing with thifluzamide (FS). These results indicate that thifluzamide has enormous potential for controlling banded leaf sheath blight in maize.

show abstract

Morphological and molecular variability among Indian isolates of Rhizoctonia solani causing banded leaf and sheath blight in maize

Cited by 16 publications

References 24 publications

Morphological and Molecular Characterization of Rhizoctonia solani causing Sheath Blight in Rice

Morphological and Molecular Characterization of Rhizoctonia solani causing Sheath Blight in Rice

Effect of photoperiod on the cultural morphology of Rhizoctonia solani isolates of maize from Yogyakarta and Central Java, Indonesia

Establishment of baseline sensitivity ofRhizoctonia solanito thifluzamide in maize and its field application

Contact Info

Product

Resources

About