Morphological and Molecular Variability in Weedy Rices of Haryana

Choudhary, N.; Ahuja, Uma; Chawla, Veena; Jain, R. K.; Kumari, Puja; Batan, K.R.

doi:10.3923/ajar.2011.250.259

Cited by 10 publications

(5 citation statements)

References 4 publications

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“…Heterozygosity was also identified in a number of SSR loci in these mutants, suggesting the segregated soybean mutant lines observed in this study. Unique alleles existed in lines of M5, and M15 on specific loci, allowing to determine DNA fingerprint for its genetic identity (Choudhary et al 2011;Lestari et al 2016). The PIC (Polymorphism Information Content) reflecting the polymorphism and allele frequency among lines, were not uniform and ranged from 0.77 (GmES1424) to 0.96 (Satt197) with an average of 0.90.…”

Section: Molecular Diversity Among Soybean Mutantsmentioning

confidence: 99%

Evaluation of SSR and important agronomical characters of promising mutant lines of soybean

et al. 2019

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Abstract. Asadi, Dewi N, Nugroho K, Terryana RT, Mastur, Lestari P. 2020. Evaluation of SSR and important agronomical characters of promising mutant lines of soybean. Biodiversitas 21: 299-310. Improved soybean (Glycine max (L.) Merr.) varieties resistant to major pest or disease, and in accordance with consumer preferences are important in breeding programs to raise their productivity. Identification of superior promising mutant lines of soybean before releasing them needs multiple environment trials complemented with molecular assay. This study aimed to assess morpho-agronomical and molecular characters using SSR markers of promising mutant lines of soybean (Glycine max (L.) Merr.). A total of 14 SSR markers were used to evaluate 20 mutant lines along with their parental lines and check varieties, and eight different locations were chosen to field evaluation of 11 selected lines induced by gamma-ray. Values of Polymorphism Information Content, allele number, and gene diversity index were high, indicating the great genetic diversity among these mutant lines, and far distant from their parental lines. Phylogenetic tree also supported the distinguishable among gamma ray-induced mutant lines compared to the parental lines. The significant interaction between promising line and environment showed their high adaptability and stable yield in various environments. Biosoy-8 (2.713 ton/ha) and Biosoy-11 (2.631 ton/ha) revealing the high yields lines supported with the molecular information could be potential to be released as new varieties and can direct their efficient utilization for field application or further improvement scheme.

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Section: Molecular Diversity Among Soybean Mutantsmentioning

confidence: 99%

Evaluation of SSR and important agronomical characters of promising mutant lines of soybean

et al. 2019

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“…Kumar and Bhagwat (2012) described that distinct alleles were identi ed for 18 rice mutants either in a single or combination of two SSRs markers. A sum of 19 unique alleles was detected from 17 genotypes of weedy rice with the size range of 200 to1300bp (Choudhary et al 2011). Results of Gealy et al (2009) explained that presence or absence of discrete SSRs loci was exploited to differentiate white rice from US weedy rice.…”

Section: P P a A A A A A A A A P A A P A A P P A P A A A A M 7 8 A A mentioning

confidence: 99%

Detection of genetic divergence among Putative Ethyl Methane Sulfonate Mutants of Super Basmati using Microsatellite Markers

Hameed¹,

Sadia²,

Habib³

et al. 2020

Preprint

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Mutation is a resource of generating genetic diversity in crop plant for breeding as well as genetic analysis. Ethyl Methane Sulphonate (EMS) is frequently applied chemical agents in plants. The present study was completed to investigate the mutagenic effects of different concentration of EMS on yield contributing traits of super basmati. The seeds of rice cultivar (Super basmati) were mutagenized with different doses from 0-2% v/v of EMS with difference of 0.25% for determination LD50. The treated and non-treated plants were observed under different agronomic parameters. A total of 48 putative EMS mutants of super basmati were selected randomly to analyze genetic diversity using 25 SSR primers. These markers were located on twelve chromosomes of rice. SSRs analysis revealed that abroad level of genetic diversity was existed among mutants of super basmati. A sum of 91 alleles was identified of which 82 alleles were originated to be polymorphic and the rest of nine alleles were discovered as monomorphic. The range of allele number was 2-10 with mean of 3.64 alleles/locus. The value of polymorphic information content (PIC) was from 0.039 (RM5) to 0.878 (RM44) with mean of 0.439 for each locus. All mutants differentiated from each other in more than two set of primers due to presence or absence of unique bands on chromosomes at definite base pair. The size base pair range was 75-1000bp. Dendrogram located mutant indices into four major groups. Phylogenic analyses exposed 40-96% similarity. It is concluded that EMS induced genetic variability and SSRs markers (RM44, RM154, RM1, RM252, RM334, RM487, RM110 and RM257) could be employed for the selection of rice mutants throughout molecular breeding program.

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“…The genetic diversity of plants has traditionally been observed based on morphological characters or growth responses. In this method, individuals are differentiated based on their phenotypic character, which can be influenced by the environment [8]. The development of molecular markers provides a sensitive method for detecting diversity at the DNA level.…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity of Dacrycarpus imbricatus At Bukit Tapak, Tabanan, Bali Based on RAPD Marker

Pharmawati

Wijaya

2021

Biotropika

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Dacrycarpus imbricatus Blume is a member of the Podocarpaceae family. In Bali, D. imbricatus was found in Bukit Tapak, Tabanan Regency. This species is one of the dominant species in Bukit Tapak. This study aimed to determine the genetic variation of D. imbricatus in Bukit Tapak using molecular markers RAPD (Random Amplified Polymorphic DNA). The genetic diversity of D. imbricatus needs to be studied to obtain the information used for the conservation of this species. Leaf samples were taken from Bukit Tapak, Candikuning, Baturiti, Tabanan Regency, Bali. DNA was extracted using the CTAB method, followed by extraction using chloroform: isoamyl alcohol. DNA precipitation was carried out using ethanol. RAPD analysis was performed using polymerase chain reaction (PCR) using four primers. PCR products were visualized using agarose gel electrophoresis and ethidium bromide staining. The results showed that the amplified DNA bands ranged from 1 to 5 bands with DNA band sizes ranging from 230 bp -422 bp. Only OPA4 and UBC106 primers can be used to detect D. imbricatus diversity based on the H, D, and R values. The detected genetic variation is low, as indicated by an average polymorphism of 32.5% and similarities between samples ranging from 0.51 to 1.

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Morphological and Molecular Variability in Weedy Rices of Haryana

Cited by 10 publications

References 4 publications

Evaluation of SSR and important agronomical characters of promising mutant lines of soybean

Evaluation of SSR and important agronomical characters of promising mutant lines of soybean

Detection of genetic divergence among Putative Ethyl Methane Sulfonate Mutants of Super Basmati using Microsatellite Markers

Genetic Diversity of Dacrycarpus imbricatus At Bukit Tapak, Tabanan, Bali Based on RAPD Marker

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