Morphological and morphometric evaluation of silver barb, Barbodes gonionotus (Bleeker, 1849) sperm supplemented with antibiotics

Abstract

Aim of this study was to evaluate sperm morphology of silver barb, Barbodes gonionotus, sperm and describe the effect of antibiotics on morphological characteristics of the sperm using an ASMA plug-in. The experiment was done at the room temperature (25°C) and divided into four treatments in three replicates: (i) freshly collected semen, (ii) extended semen (control), (iii) extended semen supplemented with 0.5% penicillin-streptomycin (PS), and (iv) extended semen supplemented with 0.5% penicillin-gentamicin (PG). Silver barb sperm comprised three main compartments: a circular head with no acrosome, a midpiece, and a single flagellum. Addition of 0.5% PS had no detrimental effects on sperm morphometry, except flagellum width. Administration of 0.5% PG affected sperm morphology in two distinct ways: (i) intact sperm (76.92 AE 5.84% of total sperm) except for flagellum width, and (ii) severe morphological damage (23.08 AE 2.67%).

Larval fish assemblage patterns in three tributaries of Mekong River in Thailand

“…Most common were small grey fishes with protracted spawning periods. These small grey fishes as well as the medium sized cyprinids such as Hampala spp., Henichorhynchus spp., Barbonymus spp., are known to start migration from the Mekong into the tributaries during February and March, then mature and spawn as early as the water level starts rising in May (Warren et al, 1998;Suntornratana et al, 2002;Baran, 2006;Boonthai et al, 2016).…”

EFFECT OF DIETARY PAPAYA PEEL AND BAKER’S YEAST SUPPLEMENTATION ON GROWTH PERFORMANCE, HEMATOLOGICAL VALUE OF SILVER BARB (Barbodes gonionotus) AND ITS RESISTANCE TO Aeromonas hydrophila

“…The bacteria is usually transmitted after insemination of the fish eggs with tainted cryopreserved sperm, which leads to reduced fertilization capacity as the embryos die and egg production gets disrupted. (Boonthai et al, 2016;Boonthai et al, 2018).…”