Morphological and phagocytic characteristics of peritoneal exudate cells in tilapia, Oreochromis niloticus (Trewavas), and carp, Cyprinus carpio L.

Abstract

The morphology and phagocytic activity of peritoneal exudate cells (PEC) obtained by an intraperitoneal injection of liquid paraffin into tilapia, Oreochromis niloticus, and carp, Cyprinus carpio, were studied with light and electron microscopy. PEC consisted of monocyte-macrophage series cells (M-MB), neutrophils, eosinophils (granular cells) and others. Cells exhibiting the same morphology as mammalian macrophages but different from monocytes of the same species were identified with light and electron microscopy and designated as peritoneal macrophages. Light and electron microscopy revealed that M-Me, neutrophils and eosinophils (granular cells) phagocytozed foreign materials added in vivo and in virro. Eosinophils appeared later in the peritoneal exudate and less actively phagocytic as compared with M-M0 and neutrophils. Small and large phagosomes were formed in M-M0, neutrophils and eosinophils (granular cells). Large phagosomes were common in neutrophils. Fusion of cytoplasmic granules with the phagosome membrane was observed. The in vitro experiment on phagocytosis revealed that the phagocytic rates in M-Ms and neutrophils were positively correlated with thedoses of foreign materials. The results indicated that these two cell types have the highest capacity of phagocytosis.,

Características hematológicas de teleósteos brasileiros: II. Parâmetros sangüíneos do Piaractus mesopotamicus Holmberg (Osteichthyes, Characidae) em policultivo intensivo

“…1985;KAJIGA YA et aI. 1985;MATUSHIMA & MARIANO 1996) e são as células de maior prevalência no exsudato inflamatório de Oreochromis niloticus Trewavas 1983 (Cichlidae), com clara evidência de atividade fagocítica (SUZUKI 1986;MATUSHIMA & MARIANO (996). Por isso não podem ser considerados responsáveis somente pelo processo de coagulação.…”

Seabream (Sparus aurata) long-term dominant-subordinate interplay affects phagocytosis by peritoneal cavity cells

“…Peritoneal cavity cells (PCCs) play important roles in both non-specific and specific immune mechanisms (Ellis, 1982;Sakai, 1984;Suzuki, 1986;Olivier et al, 1992), and the peritoneal exudate has been used as a source of leucocytes for studies of innate immunity, including phagocytosis (Bodammer, 1986;Vazzana et al, 2003) and cytotoxicity (Cammarata et al, 2000).…”

Characterization of the acute inflammatory response in the hybrid tambacu (Piaractus mesopotamicus male × Colossoma macropomum female) (Osteichthyes)

“…Later, the inflammatory response was evaluated by ink injection (Ellis et al, 1976), complete Freund's adjuvant and Staphylococcus aureus (Finn and Nielsen, 1971), carrageenin (Timur et al, 1977), Escherichia coli lipopolysaccharide (LPS), complete Freund's adjuvant, turpentine and carrageenin (White et al, 1981), Vibrio alginolyticus (MacArthur et al, 1984), liquid paraffin (Suzuki, 1986), Edwardsiella ictaluri LPS (Saeed and Plumb, 1986), Pseudomonas alcaligenes and Aeromonas punctata (Siwicki and Studnicka, 1987), Aeromonas salmonicida (Reite and Evensen, 1994), LPS (Brunetti et al, 1994), Freund's adjuvant and squalene (Jenkins and Klesius, 1998), microcystin (Vajcová et al, 1998), Escherichia coli , peptidoglican (Kono and Sakai, 2001), beta-glucan of Saccharomyces cerevisiae and Salmonella typhimurium LPS (Paulsen et al, 2001) and Mycobacterium marinum (Gauthier et al, 2003). In these studies, the inflammation was characterized by showing macrophages, leucocytes, fibroblasts and fibroplasia.…”