Morphological and physiological photon flux influence under in vitro culture of apple shoots

Zanandrea, Ilisandra; Bacarin, Marcos Antônio; Braga, Eugênia Jacira Bolacel; Bianchi, Valmor João; Peters, José Antônio

doi:10.1590/s1516-89132009000500005

Cited by 5 publications

(3 citation statements)

References 27 publications

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“…The plant material was dried in an oven with forced air circulation at 65°C until constant mass, crushed in a Willey mill, model STAR FT-50, and stored in a freezer at -40°C. The extracts were obtained according to Zanandrea et al (2009), using 0.2 g of plant tissue. For the quantification of total soluble sugars and starch, the anthrone method (Yemm and Willis 1954) was used, with modifications.…”

Section: Plant Materials and Growing Conditionsmentioning

confidence: 99%

Responses of wild Piper species to drought and rehydration cycles considering stomatal closure as a marker of the alarm phase

CERRI NETO,

SILVA,

FERREIRA

et al. 2023

Photosynt.

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Abbreviations: abE -abaxial epidermis; ABS/RC -energy absorption flux per reaction center; adE -adaxial epidermis; APDM -dry mass of the aerial part; Chl -chlorophyll; Ci -internal CO2 concentration; CSVBL -cortex stem vascular bundle length; DCSVdiameter of cortex stem vessels; DI0/RC -energy flux dissipated per reaction center; E -transpiration; EDS -equatorial diameter of stomata; ET0/RC -energy transport flow per reaction center; gs -stomatal conductance; ICE -instantaneous carboxylation efficiency; LA -leaf area; LAR -leaf area ratio; LDM -leaf dry mass; LMF -leaf mass fraction; LSMVB -length of stem medulla vascular bundle; LSMX -length of stem medulla xylem; LVBL -leaf vascular bundle length; NL -number of leaves; NRV -number of root vessels; NVMS -number of vessels medulla stem; NXV -number of xylem vessels; PDS -polar diameter of stomata; PI(abs) -performance index for conservation of energy from captured excitons to reduction of intersystem electron acceptors; PN -photosynthetic rate; PPTpalisade parenchyma thickness; RC/CS0 -number of active PSII reaction centers per cross sections; RDM -root dry mass; Rep -root epidermis; RI -robustness index; RMF -root mass fraction; R/SR -root/shoot ratio; RS -reducing sugar; RTD -root tissue density; RV -root volume; RVD -root vessel diameter; RXL -root xylem length; SD -stem diameter; Sd -stomatal density; SL -stem length; SMF -stem mass fraction; SPT -spongy parenchyma thickness; SRL -specific root length; TDM -total dry mass; TR0/RC -energy flow captured by reaction center; TSS -total soluble sugar; WUE -water-use efficiency; XCSL -xylem cortex stem length; φE0quantum efficiency of electron transfer from QAto the electron transport chain beyond QA -; φP0 -maximum photochemical quantum yield; Ψ -leaf water potential.

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Section: Plant Materials and Growing Conditionsmentioning

confidence: 99%

Responses of wild Piper species to drought and rehydration cycles considering stomatal closure as a marker of the alarm phase

CERRI NETO,

SILVA,

FERREIRA

et al. 2023

Photosynt.

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“…The allocation of carbohydrates was evaluated through the quantification of reducing sugars (RS), total soluble sugars (TSS), and leaf starch, stem above and below the grafting point and roots. The plant tissues used for dry mass evaluation were ground in a Willie-knife mill, model STAR FT-50, stored in a freezer at -18 ° C. The extracts were obtained according to Zanandrea et al (2009), using variable mass, 0.15 g (leaf and stem below the grafting point); 0.1 g (stem below the grafting point), 0.3 g (cultivar root), and 0.4 g (wild plants root).…”

Section: Analysis Of Carbohydratesmentioning

confidence: 99%

Primary metabolism and initial development of grafted black pepper seedlings

Crasque¹,

Arantes²,

Neto³

et al. 2021

RSD

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The production of black pepper seedlings through grafting is a promising strategy to mitigate the effect of environmental stresses, such as drought, that limit its cultivation. However, studies on compatibility between grafts and rootstocks are still scarce, especially considering the wild species of the genus Piper and the cultivated genotypes of black pepper. The objective of this work was to evaluate the relationship between primary metabolism and the development of black pepper seedlings obtained through intra- and interspecific grafting. The method used was that of cleft lateral grafting, as graft the Bragantina cultivar, better known in the world as ‘Panniyur 1’, and ‘Kottanadan’ cultivar and three wild species (Piper hispidum, Piper aduncum and Piper tuberculatum) were used as root-stock. As a control, ´Bragantina` was grafted onto itself (homograft). The experiment was carried out for 110 days and the seedlings were evaluated for development, gas exchange, and carbohydrate allocation. The rootstock influences the development and primary metabolism of seedlings. P. aduncum showed greater initial compatibility among wild species, due to less impediment to carbohydrate flow. P. tuberculatum showed early incompatibility, as the grafts died. The seedlings grafted on P. hispidum and 'Kottanadan' had initial compatibility with an average of 78% survival and 60% budding, but they may have late incompatibility due to limitation of carbohydrate flow.

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“…Отже, шляхом вимірювання виходу флуоресценції можна отримати інформацію про зміни ефективності фотосинтезу та розсіювання тепла [3,6]. Флуоресцентний аналіз хлорофілу є одним із найпотужніших і широко використовуваних методів вивчення впливу умов зовнішнього середовища на процес фотосинтезу рослин, що культивуються в умовах in vitro [7].…”

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Dependency of chlorophyll fluorescence of in vitro plant of Carlina L. on light conditions during their cultivation

Kolisnyk,

Hrytsak,

Prokopiak

et al. 2023

Fakt. eksp. evol. org.

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Aim. To investigate the peculiarities of the functioning of the photosynthetic apparatus of plants of Carlina L. species under different light conditions of in vitro cultivation and under natural conditions using the method of induction of fluorescence of chlorophyll a. Methods. Methods of in vitro plants cultivation, a method of inducing fluorescence of chlorophyll a in light-adapted leaves. Results. It has been demonstrated that in vitro conditions reflect the evolved light growth requirements of species. Our previously obtained results regarding a closer match of physiological needs of in vitro plants of the species of Carlina onopordifolia Besser ex Szafer, Kulcz. et Pawt. to light conditions of variant 1.1 have been confirmed. Based on the synthesis of the dynamics of photosynthetic pigment content and their ratios in in vitro plants of Carlina acaulis L., as well as key parameters of fluorescence under different cultivation light conditions, a conclusion has been drawn about the greater alignment of variant 1.1 with the needs of plants of this species. It is hypothesized that in natural conditions, plants of the species of C. onopordifolia and Carlina cirsioides Klokov undergo abiotic stresses, manifested in increased dissipation of light energy into heat and the processes of photo-inhibition. There is significant enhancement in electron transport within the light-harvesting complex and reduction in the efficiency of quantum yield and light energy storage by Photosystem II. The vitality index of these species in their natural growth conditions is found to be half of the commonly accepted optimum value and 1.97–2.96 times lower compared to experimental in vitro plant groups. Conclusions. The obtained results suggest the usefulness of using the chlorophyll fluorescence induction method for assessing the functioning of the photosynthetic apparatus of in vitro plants of the genus Carlina.

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Morphological and physiological photon flux influence under in vitro culture of apple shoots

Abstract: The aim of the present study was to evaluate the growth and development of apple rootstock shoots submitted to different photon flux densities (7, 14, 21

Cited by 5 publications

References 27 publications

Responses of wild Piper species to drought and rehydration cycles considering stomatal closure as a marker of the alarm phase

Responses of wild Piper species to drought and rehydration cycles considering stomatal closure as a marker of the alarm phase

Primary metabolism and initial development of grafted black pepper seedlings

Dependency of chlorophyll fluorescence of in vitro plant of Carlina L. on light conditions during their cultivation

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