Morphological and protein alterations in Sclerotinia sclerotiorum (Lib.) de Bary after exposure to volatile organic compounds of Trichoderma spp.

Silva, Lincon Rafael da; Valadares-Inglis, M. C.; Moraes, M. C. B.; Magalhães, Diego Martins; Sifuentes, Daniel Nogoceke; Martins, Irene; Mello, S. C. M.

doi:10.1016/j.biocontrol.2020.104279

Cited by 15 publications

(4 citation statements)

References 29 publications

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“…It has also been reported that VOCs from Trichoderma spp. reduce the width and decrease the density of hyphae of S. sclerotiorum ( Motlagh and Abolghasemi, 2022 ; da Silva et al, 2020 ), and those of T. asperellum cause anomalies in the hyphae of Fusarium incarnatum ( Intana et al, 2021 ). From these results, it is postulated that VOCs can weaken the pathogen and hinder the infective process in plants and, consequently, reduce the severity of the disease.…”

Section: Discussionmentioning

confidence: 99%

“…However, until the execution of this study, no reports had been found on the action of Trichoderma VOCs in five different periods of cultivation of the biocontrol agent. The choice of growth periods of 0, 24, 48, 72, and 96 h was inferred from previous experiments, in which the doubleplate confrontation between T. azevedoi CEN1241 and S. sclerotiorum CEN1147 lasted approximately 72 to 96 h, compared to the control treatment, colonizing the entire diameter of the Petri dish (da Silva et al, 2020(da Silva et al, , 2021a.…”

Section: Discussionmentioning

confidence: 99%

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Colony Age of Trichoderma azevedoi Alters the Profile of Volatile Organic Compounds and Ability to Suppress Sclerotinia sclerotiorum in Bean Plants

Silva

Rodrigues

Botelho³

et al. 2023

Plant Pathol J

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Common bean (Phaseolus vulgaris L.) is one of the most important crops in human food production. The occurrence of diseases, such as white mold, caused by Sclerotinia sclerotiorum can limit the production of this legume. The use of Trichoderma has become an important strategy in the suppression of this disease. The aim of the present study was to evaluate the effect of volatile organic compounds (VOCs) emitted by Trichoderma azevedoi CEN1241 in five different growth periods on the severity of white mold in common bean. The in vitro assays were carried out in double-plate and split-plate, and the in vivo assays, through the exposure of the mycelia of S. sclerotiorum to the VOCs of T. azevedoi CEN1241 and subsequent inoculation in bean plants. Chemical analysis by gas chromatography coupled to mass spectrometry detected 37 VOCs produced by T. azevedoi CEN1241, covering six major chemical classes. The profile of VOCs produced by T. azevedoi CEN1241 varied according to colony age and was shown to be related to the ability of the biocontrol agent to suppress S. sclerotiorum. T. azevedoi CEN1241 VOCs reduced the size of S. sclerotiorum lesions on bean fragments in vitro and reduced disease severity in a greenhouse. This study demonstrated in a more applied way that the mechanism of antibiosis through the production of volatile compounds exerted by Trichoderma can complement other mechanisms, such as parasitism and competition, thus contributing to a better efficiency in the control of white mold in bean plants.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Colony Age of Trichoderma azevedoi Alters the Profile of Volatile Organic Compounds and Ability to Suppress Sclerotinia sclerotiorum in Bean Plants

Silva

Rodrigues

Botelho³

et al. 2023

Plant Pathol J

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“…Bacillus spp. attach to mycelial cell walls and deform hyphae through the production of extracellular enzymes such as chitosanase, protease, glucanase, and cellulase [ 5 , 39 ]. Lipopeptides, including fengycin, iturin, pumilacidin, mixirin, and surfactin, are antifungal peptides that function against pathogenic fungi in rhizospheres [ 1 , 3 ].…”

Section: Beneficial Bacteria and Crop Disease Managementmentioning

confidence: 99%

Bacterial and Fungal Biocontrol Agents for Plant Disease Protection: Journey from Lab to Field, Current Status, Challenges, and Global Perspectives

Ayaz,

Li,

Ali

et al. 2023

Molecules

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Plants are constantly exposed to various phytopathogens such as fungi, Oomycetes, nematodes, bacteria, and viruses. These pathogens can significantly reduce the productivity of important crops worldwide, with annual crop yield losses ranging from 20% to 40% caused by various pathogenic diseases. While the use of chemical pesticides has been effective at controlling multiple diseases in major crops, excessive use of synthetic chemicals has detrimental effects on the environment and human health, which discourages pesticide application in the agriculture sector. As a result, researchers worldwide have shifted their focus towards alternative eco-friendly strategies to prevent plant diseases. Biocontrol of phytopathogens is a less toxic and safer method that reduces the severity of various crop diseases. A variety of biological control agents (BCAs) are available for use, but further research is needed to identify potential microbes and their natural products with a broad-spectrum antagonistic activity to control crop diseases. This review aims to highlight the importance of biocontrol strategies for managing crop diseases. Furthermore, the role of beneficial microbes in controlling plant diseases and the current status of their biocontrol mechanisms will be summarized. The review will also cover the challenges and the need for the future development of biocontrol methods to ensure efficient crop disease management for sustainable agriculture.

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“…VOCs released by Trichoderma include lactones, ketones, alcohols, mono- and sesquiterpenes, esters and aldehydes, all of them with selective bioactivity [ 21 , 22 ]. Several investigations have demonstrated the antimicrobial properties of Trichoderma VOCs [ 23 , 24 , 25 , 26 , 27 , 28 ] as well as their role in the induction of defenses and/or growth promotion in plants [ 19 , 22 , 29 , 30 , 31 , 32 , 33 , 34 ]. Production of VOCs in Trichoderma is directly dependent not only on the species, but also on the strain, and it is also affected by growth conditions, developmental stage and the abiotic or biotic cues received from the environment [ 21 , 35 , 36 , 37 , 38 ].…”

Section: Introductionmentioning

confidence: 99%

Trichoderma harzianum Volatile Organic Compounds Regulated by the THCTF1 Transcription Factor Are Involved in Antifungal Activity and Beneficial Plant Responses

Rubio

Monti

Gualtieri

et al. 2023

JoF

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The transcription factor THCTF1 from Trichoderma harzianum, previously linked to the production of 6-pentyl-2H-pyran-2-one (6-PP) derivatives and antifungal activity against Fusarium oxysporum, has been related in this study to conidiation, production of an array of volatile organic compounds (VOCs) and expression of methyltransferase genes. VOCs emitted by three T. harzianum strains (wild type T34, transformant ΔD1-38 that is disrupted in the Thctf1 gene encoding the transcription factor THCTF1, and ectopic integration transformant ΔJ3-16) were characterized by Proton Transfer Reaction-Quadrupole interface-Time-Of-Flight-Mass Spectrometry (PTR-Qi-TOF-MS). Thctf1 disruption affected the production of numerous VOCs such as the antifungal volatiles 2-pentyl furan and benzaldehyde which were under-emitted, and acetoine, a plant systemic defense inductor, which was over-emitted. Biological assays show that VOCs regulated by THCTF1 are involved in the T. harzianum antifungal activity against Botrytis cinerea and in the beneficial effects leading to Arabidopsis plant development. The VOC blend from the disruptant ΔD1-38: (i) inhibited Arabidopsis seed germination for at least 26 days and (ii) when applied to Arabidopsis seedlings resulted in increased jasmonic acid- and salicylic acid-dependent defenses.

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Morphological and protein alterations in Sclerotinia sclerotiorum (Lib.) de Bary after exposure to volatile organic compounds of Trichoderma spp.

Cited by 15 publications

References 29 publications

Colony Age of Trichoderma azevedoi Alters the Profile of Volatile Organic Compounds and Ability to Suppress Sclerotinia sclerotiorum in Bean Plants

Colony Age of Trichoderma azevedoi Alters the Profile of Volatile Organic Compounds and Ability to Suppress Sclerotinia sclerotiorum in Bean Plants

Bacterial and Fungal Biocontrol Agents for Plant Disease Protection: Journey from Lab to Field, Current Status, Challenges, and Global Perspectives

Trichoderma harzianum Volatile Organic Compounds Regulated by the THCTF1 Transcription Factor Are Involved in Antifungal Activity and Beneficial Plant Responses

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