Morphological and quantitative study of the myenteric plexus of the ascending colon of rats subjected to proteic desnutrition

Sant’Ana, Débora de Mello Gonçales; Miranda-Neto, Marcílio Hubner de; Souza, Romeu Rodrigues de; Molinari, Sônia Lucy

doi:10.1590/s0004-282x1997000500003

Cited by 44 publications

(72 citation statements)

References 13 publications

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“…The importance of proteins in the processes of synthesis of substances for growth and tissue re-newal was demonstrated by the lower weight gain and smaller bowels of the experimental animals, similar to the findings in rats with protein desnutrition 16 .…”

Section: Discussionsupporting

confidence: 78%

“…Therefore, about 50.5% of the nitrergic neurons and 27.2% of the NADH-diaphorase positive neurons of the ascending colon were not evidenced. Significant reductions in the number of myenteric neurons of malnourished rats were also verified in other investigations, and have been attributed to the nutritional deficiency to which the animals were subjected [8][9][10][11]16 .…”

Section: Discussionmentioning

confidence: 55%

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Effects of protein and vitamin B deficiency on blood parameters and myenteric neurons of the colon of rats

Sant’Ana

Molinari

Miranda-Neto

2001

Arq. Neuro-Psiquiatr.

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-The aims of this work were to evaluate the effects of the deficient ingestion of protein and vitamin B on the biochemical and hematologic parameters and on the NADH-and NADPH-diaphorase positive myenteric neurons. The control animals (n=10) received commercial chow and the experimental rats (n=10) received chow with protein level reduced to 8% during 120 days. At the time of killing blood was collected for assessment of the blood and hematologic parameters and the ascending colon for quantitative analysis of the neurons of the myenteric plexus. It was observed that the reduction of the protein level to 8% coupled to the reduction of the levels of vitamin B in adult rats neither led to qualitative or quantitative changes on red or white blood cells, nor decreased globulin levels, induced the formation of edema or gave rise to clinical signs typical of protein or vitamin B deficiency. On the other hand, the experimental protocol led to less weight gain, change on the body composition with fat deposition; decrease of the values of serum total protein and albumin; reduction of the area of colon and density of nitrergic and NADH-diaphorase myenteric neurons inferior to the expected.KEY WORDS: enteric neurons, proteic desnutrition, ascending colon, hematology, vitamin B.Efeitos da deficiência de proteínas e vitamina B sobre parâmetros sanguíneos e neurônios mioentéricos do colo de ratos adultos RESUMO -Os objetivos deste trabalho foram avaliar as repercussões da ingestão deficiente de proteínas e vitaminas do complexo B sobre parâmetros bioquímicos e hematológicos e neurônios mientéricos NADH e NADPH-diaforase positivos. Os animais controle (n=10) receberam ração comercial e os animais experimentais (n=10) ração com teor protéico reduzido para 8% durante 120 dias. Durante o sacrifício, coletou-se o sangue dos animais para avaliação de parâmetros sanguíneos e hematológicos e o colo ascendente para a análise quantitativa dos neurônios do plexo mioentérico. Observou-se que a redução do teor de proteínas na dieta para 8% associado a redução no teor de vitaminas do complexo B em ratos adultos, não causa alterações qualitativas e quantitativas das células sanguíneas das séries vermelha e branca, não leva a redução do valor da globulina nem a formação de edema ou ao surgimento de sinais clínicos característicos das carências de proteínas e vitaminas do complexo B. Por outro lado causa: menor ganho de peso corporal; alteração da composição corporal com acúmulo de gordura; redução nos valores de proteína total e albumina; redução na área do colo e evidenciação de densidade de neurônios nitrérgicos e NADH-diaforase positivos inferior ao esperado.PALAVRAS CHAVE: neurônios entéricos, desnutrição protéica, colo ascendente, hematologia, vitamina B. The utilization of animal models in studies of food restriction with known and controlled diets has as an advantage the improved knowledge about the many aspects of the human desnutrition 1 . Research on desnutrition aimsat simulating natural conditions, which involve deficienc...

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Section: Discussionsupporting

confidence: 78%

Section: Discussionmentioning

confidence: 55%

Effects of protein and vitamin B deficiency on blood parameters and myenteric neurons of the colon of rats

Sant’Ana

Molinari

Miranda-Neto

2001

Arq. Neuro-Psiquiatr.

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“…The measurement of the yeast cell sizes was carried out with the aid of an Olympus CBB microscope coupled with a micrometer disc. We measured the major transverse and longitudinal axes of 100 viable cells of each preparation 19 .…”

Section: In Vitro Evaluation Of Fungal Strains Morphometrical Charactmentioning

confidence: 99%

Paracoccidioides brasilienses isolates obtained from patients with acute and chronic disease exhibit morphological differences after animal passage

Svidzinski

Neto

Santana

et al. 1999

Rev. Inst. Med. trop. S. Paulo

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SUMMARYThe basis for virulence in Paracoccidioides brasiliensis is not completely understood. There is a consensus that the sequencial in vitro subcultivation of P. brasiliensis leads to loss of its pathogenicity, which can be reverted by reisolation from animal passage. Attention to morphological and biochemical properties that are regained or demonstrated after animal passage may provide new insights into factors related to the pathogenicity and virulence of P. brasiliensis. We evaluated morphological characters: the percentage of budding cells, number of buds by cell and the diameter of 100 mother cells of yeast-like cells of 30 P. brasiliensis isolates, before and after animal passage. The isolates were obtained from patients with different clinical forms of paracoccidioidomycosis (PCM): acute form (group A, n=15) and chronic form (group C, n=15). The measurement of the yeast cell sizes was carried out with the aid of an Olympus CBB microscope coupled with a micrometer disc. We measured the major transverse and longitudinal axes of 100 viable cells of each preparation. The percentage of budding cells as also the number of buds by cell was not influenced by animal passage, regardless of the source of the strain (acute or chronic groups). The size values of P. brasiliensis isolates from groups A and C, measured before the animal passage exhibited the same behavior. After animal passage, there was a statistically significant difference between the cell sizes of P. brasiliensis isolates recovered from testicles inoculated with strains from groups A and C. The maximum diameter of mother cells from group A isolates exhibited a size of 42.1µm in contrast with 32.9µm exhibited by mother cells from group C (p<0.05). The diameter of 1500 mother cells from group A isolates exhibited a medium size of 16.0µm (SD ± 4.0), a value significantly higher than the 14.1µm (SD = ± 3.3) exhibited by 1500 mother cells from group C isolates (p<0.05). Our results reinforce the polymorphism exhibited by P. brasiliensis in biological material and the need for further investigations to elucidate the role of morphological parameters of the fungus in the natural history of the disease.

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“…Myenteric neurons were considered large when the areas of cell body profiles were above the mean+SD, small when the areas were below the mean-SD and medium when the areas were on the interval of the mean±SD. This procedure of classification was the same as that employed in other morphometric analyses previously carried out in our laboratory 5,6,8 .…”

Section: Discussionmentioning

confidence: 99%

“…The morphoquantitative studies of the myenteric plexus carried out until the 80s used to consider a given segment of the digestive tube, but recently it was found that different regions of the stomach and of the intestinal circumference also differ in their features and in their sensitivity to experimental procedures [4][5][6] . This fact deserves attention because phylogenetic, age-related and pathophysiological comparisons can be interpreted erroneously due to the random selection of neurons around the intestinal circumference.…”

mentioning

confidence: 99%

Evaluation of the areas of neuronal cell bodies and nuclei in the myenteric plexus of the duodenum of adult rats

Miranda-Neto

Furlan

Sant’Ana

et al. 2000

Arq. Neuro-Psiquiatr.

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-This study compared the areas of cell body and nucleus profiles of the myenteric neurons in the antimesenteric and intermediate regions of the duodenum of adult rats. Five male rats were used. The duodenum was removed and dissected to whole-mount preparations, which were stained by the Giemsa technique. The areas of cell body and nucleus profiles of 100 neurons, 50 from each region, of each animal, were assessed with image analyser. Based on the global mean±SD of the areas of cell body profiles, neurons were labelled as small, medium or large. It was observed that the neurons did not differ significantly in size or incidence between the antimesenteric and intermediate regions. However, the nuclei of the small and medium neurons were significantly smaller in the latter region. It is discussed that the smaller nuclear size could be related to the cell bodies being slightly smaller on this region and to a possible smaller biosynthetic activity which would influence nuclear size.KEY WORDS: myenteric neurons, neuronal area, duodenum, rats. Avaliação das áreas dos corpos celulares e dos núcleos neuronais no plexo mientérico do duodeno de ratos adultosRESUMO -Este estudo comparou as áreas dos perfis dos corpos celulares e dos núcleos neuronais dos neurônios mientéricos nas regiões antimesentérica e intermediária do duodeno de ratos adultos. Cinco ratos machos foram usados. O duodeno foi removido e dissecado a preparados de membrana, os quais foram corados pela técnica de Giemsa. As áreas dos perfis dos corpos celulares e dos núcleos neuronais de 100 neurônios, 50 de cada região, de cada animal, foram avaliadas com analisador de imagens. Com base na média global±SD das áreas dos perfis dos corpos celulares, os neurônios foram classificados como pequenos, médios ou grandes. Foi observado que os neurônios não diferiram significativamente de tamanho ou incidência entre as regiões antimesentérica e intermediária. Contudo, os núcleos dos neurônios pequenos e médios foram significativamente menores na última. Discute-se que o menor tamanho nuclear poderia estar relacionado ao fato dos corpos celulares serem ligeiramente menores nesta região e a uma atividade biossintética possivelmente menor, que influenciaria o tamanho nuclear. PALAVRAS-CHAVE: neurônios mientéricos, área neuronal, duodeno, ratos.For many years the enteric nervous system (ENS) has been the goal of studies aiming, first, at unravelling its intricate structure, neuronal cell types, connections and neurochemical coding and, second, at understanding how these aspects are affected by factors such as age and pathophysiological Trabalho resultante de pesquisa realizada durante o mestrado de MMDP Furlan pelo

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Morphological and quantitative study of the myenteric plexus of the ascending colon of rats subjected to proteic desnutrition

Cited by 44 publications

References 13 publications

Effects of protein and vitamin B deficiency on blood parameters and myenteric neurons of the colon of rats

Effects of protein and vitamin B deficiency on blood parameters and myenteric neurons of the colon of rats

Paracoccidioides brasilienses isolates obtained from patients with acute and chronic disease exhibit morphological differences after animal passage

Evaluation of the areas of neuronal cell bodies and nuclei in the myenteric plexus of the duodenum of adult rats

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