Morphological, Biochemical, and Proteomic Analyses to Understand the Promotive Effects of Plant-Derived Smoke Solution on Wheat Growth under Flooding Stress

Komatsu, Setsuko; Yamaguchi, Hisateru; Hitachi, Keisuke; Tsuchida, Kunihiro; Rehman, Shafiq Ur; Ohno, Toshihisa

doi:10.3390/plants11111508

Cited by 10 publications

(5 citation statements)

References 59 publications

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“…In flooding conditions, the energy to maintain plant vitality mainly relies on the ethanol-metabolic pathway in glycolysis to degrade glucose and glycogen accompanied by ATP generation [ 24 ]. Under flooding stress, a plant-derived smoke solution enhanced wheat growth; and FBA/GAPDH among glycolysis, which increased under flooding, decreased with its application under the same condition [ 25 ]. In this study, FBA was accumulated in root/leaf under flooding; and its accumulation was recovered to the control level in irradiated wheat ( Figure 5 ).…”

Section: Discussionmentioning

confidence: 99%

Proteomic and Biochemical Approaches Elucidate the Role of Millimeter-Wave Irradiation in Wheat Growth under Flooding Stress

Komatsu

Tsutsui

Furuya

et al. 2022

IJMS

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Flooding impairs wheat growth and considerably affects yield productivity worldwide. On the other hand, irradiation with millimeter waves enhanced the growth of chickpea and soybean under flooding stress. In the current work, millimeter-wave irradiation notably enhanced wheat growth, even under flooding stress. To explore the protective mechanisms of millimeter-wave irradiation on wheat under flooding, quantitative proteomics was performed. According to functional categorization, proteins whose abundances were changed significantly with and without irradiation under flooding stress were correlated to glycolysis, reactive-oxygen species scavenging, cell organization, and hormonal metabolism. Immunoblot analysis confirmed that fructose-bisphosphate aldolase and β tubulin accumulated in root and leaf under flooding; however, even in such condition, their accumulations were recovered to the control level in irradiated wheat. The abundance of ascorbate peroxidase increased in leaf under flooding and recovered to the control level in irradiated wheat. Because the abundance of auxin-related proteins changed with millimeter-wave irradiation, auxin was applied to wheat under flooding, resulting in the application of auxin improving its growth, even in such condition. These results suggest that millimeter-wave irradiation on wheat seeds improves the recovery of plant growth from flooding via the regulation of glycolysis, reactive-oxygen species scavenging, and cell organization. Additionally, millimeter-wave irradiation could promote tolerance against flooding through the regulation of auxin contents in wheat.

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Section: Discussionmentioning

confidence: 99%

Proteomic and Biochemical Approaches Elucidate the Role of Millimeter-Wave Irradiation in Wheat Growth under Flooding Stress

Komatsu

Tsutsui

Furuya

et al. 2022

IJMS

Self Cite

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“…Among the modulated proteins, fructose‐bisphosphate aldolase, the key enzyme of glycolysis and gluconeogenesis, was strongly upregulated (Komatsu et al . 2022). A similar pattern was observed in model plants subjected to flooding (Khatoon et al .…”

Section: Discussionmentioning

confidence: 99%

“…2012; Komatsu et al . 2022) and in Acanthus mollis and A. ilicifolius (Liu & Zheng 2021). In these studies, the authors suggested that plants acclimate to chronic hypoxic stress by activating glycolysis and gluconeogenesis to generate energy for survival.…”

Section: Discussionmentioning

confidence: 99%

Physiology and proteomics analyses reveal the response mechanisms of Rhizophora mucronata seedlings to prolonged complete submergence

et al. 2023

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Mangrove seedlings are subject to natural tidal inundation, while occasional flooding may lead to complete submergence. Complete submergence reduces light availability and limits gas exchange, affecting several plant metabolic processes. The present study focuses on Rhizophora mucronata, a common mangrove species found along the coasts of Thailand and the Malay Peninsula.• To reveal response mechanisms of R. mucronata seedlings to submergence, a physiological investigation coupled with proteomic analyses of leaf and root tissues was carried out in plants subjected to 20 days of control (drained) or submerged conditions.• Submerged seedlings showed decreased photosynthetic activity, lower stomatal conductance, higher total antioxidant capacity in leaves and higher lipid peroxidation in roots than control plants. At the same time, tissue nutrient ion content displayed organ-specific responses. Proteome analysis revealed a significant change in 240 proteins in the leaves and 212 proteins in the roots. In leaves, most differentially accumulated proteins (DAPs) are associated with nucleic acids, stress response, protein transport, signal transduction, development and photosynthesis. In roots, most DAPs are associated with protein metabolic process, response to abiotic stimulus, nucleic acid metabolism and transport.• Our study provides a comprehensive understanding of submergence responses in R. mucronata seedlings. The results suggest that submergence induced multifaceted stresses related to light limitation, oxidative stress and osmotic stress, but the responses are organ specific. The results revealed many candidate proteins which may be essential for survival of R. mucronata under prolonged submergence.

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“…Stable isotope labelling by/with amino acids in cell culture (SILAC) and isobaric tags for absolute and relative quantification (iTRAQ) seem to be more sophisticated techniques that may monitor changes in the specific kinase domain. So far, various proteomic studies revealed abiotic stress tolerance of the plant, such as in drought [ 167 , 168 ], heat [ 158 , 169 ], chilling [ 159 , 170 ], salinity [ 156 , 171 ] and waterlogging [ 172 , 173 ]. Comparative proteome analysis for Medicago sativa cv.…”

Section: Proteomicsmentioning

confidence: 99%

Multi-Omics Pipeline and Omics-Integration Approach to Decipher Plant’s Abiotic Stress Tolerance Responses

Roychowdhury

Das²,

Gupta

et al. 2023

Genes

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The present day’s ongoing global warming and climate change adversely affect plants through imposing environmental (abiotic) stresses and disease pressure. The major abiotic factors such as drought, heat, cold, salinity, etc., hamper a plant’s innate growth and development, resulting in reduced yield and quality, with the possibility of undesired traits. In the 21st century, the advent of high-throughput sequencing tools, state-of-the-art biotechnological techniques and bioinformatic analyzing pipelines led to the easy characterization of plant traits for abiotic stress response and tolerance mechanisms by applying the ‘omics’ toolbox. Panomics pipeline including genomics, transcriptomics, proteomics, metabolomics, epigenomics, proteogenomics, interactomics, ionomics, phenomics, etc., have become very handy nowadays. This is important to produce climate-smart future crops with a proper understanding of the molecular mechanisms of abiotic stress responses by the plant’s genes, transcripts, proteins, epigenome, cellular metabolic circuits and resultant phenotype. Instead of mono-omics, two or more (hence ‘multi-omics’) integrated-omics approaches can decipher the plant’s abiotic stress tolerance response very well. Multi-omics-characterized plants can be used as potent genetic resources to incorporate into the future breeding program. For the practical utility of crop improvement, multi-omics approaches for particular abiotic stress tolerance can be combined with genome-assisted breeding (GAB) by being pyramided with improved crop yield, food quality and associated agronomic traits and can open a new era of omics-assisted breeding. Thus, multi-omics pipelines together are able to decipher molecular processes, biomarkers, targets for genetic engineering, regulatory networks and precision agriculture solutions for a crop’s variable abiotic stress tolerance to ensure food security under changing environmental circumstances.

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Morphological, Biochemical, and Proteomic Analyses to Understand the Promotive Effects of Plant-Derived Smoke Solution on Wheat Growth under Flooding Stress

Cited by 10 publications

References 59 publications

Proteomic and Biochemical Approaches Elucidate the Role of Millimeter-Wave Irradiation in Wheat Growth under Flooding Stress

Proteomic and Biochemical Approaches Elucidate the Role of Millimeter-Wave Irradiation in Wheat Growth under Flooding Stress

Physiology and proteomics analyses reveal the response mechanisms of Rhizophora mucronata seedlings to prolonged complete submergence

Multi-Omics Pipeline and Omics-Integration Approach to Decipher Plant’s Abiotic Stress Tolerance Responses

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