Morphological changes in glial cells arrangement under mechanical loading: A quantitative study

Eskandari, Faezeh; Shafieian, Mehdi; Aghdam, M.M.; Laksari, Kaveh

doi:10.1016/j.injury.2022.08.062

Cited by 2 publications

(1 citation statement)

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“…Previous studies have provided first insights into the effects of mechanical loading on the brain’s microstructure 11 . Existing investigations assessing damage mechanisms associated with traumatic brain injury (TBI) have mostly used animal models, for example performing controlled cortical impact (reviewed in 12 ), or performed sequential mechanical testing and histological examination on animal tissue 13 , 14 , in vitro cell cultures 15 , 16 , or brain organoids 17 . Bar-Kochba et al 18 simultaneously loaded and imaged neurons embedded in a 3D collagen gel.…”

Section: Introductionmentioning

confidence: 99%

Mechanisms of mechanical load transfer through brain tissue

Reiter

Paulsen

Budday

2023

Sci Rep

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Brain injuries are often characterized by diffusely distributed axonal and vascular damage invisible to medical imaging techniques. The spatial distribution of mechanical stresses and strains plays an important role, but is not sufficient to explain the diffuse distribution of brain lesions. It remains unclear how forces are transferred from the organ to the cell scale and why some cells are damaged while neighboring cells remain unaffected. To address this knowledge gap, we subjected histologically stained fresh human and porcine brain tissue specimens to compressive loading and simultaneously tracked cell and blood vessel displacements. Our experiments reveal different mechanisms of load transfer from the organ or tissue scale to single cells, axons, and blood vessels. Our results show that cell displacement fields are inhomogeneous at the interface between gray and white matter and in the vicinity of blood vessels—locally inducing significant deformations of individual cells. These insights have important implications to better understand injury mechanisms and highlight the importance of blood vessels for the local deformation of the brain’s cellular structure during loading.

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