Morphological changes in the ovarian carcinoma cells of Wistar rats induced by chemotherapy with cisplatin and dioxadet

Abstract

The development of new express methods for the analysis of the efficacy of anticancer therapy on the cellular level is highly desirable for the analysis of chemotherapeutic agent performance. In this paper we suggest the use of parameters of cell morphology determined by holographic microscopy and tomography for the effective label free quantitative analysis of cell viability under antitumor chemotherapy and thus of cytostatic agent efficacy. As shown, measured phase shifts and cell morphology change dramatically as a result of chemotherapy and depend strongly on the cell type and agent applied. Experimentally, a comparative analysis of the antitumor efficacy of the two cytostatics, cisplatin and dioxadet, that are commonly used for chemotherapy of disseminated ovarian carcinoma has been performed. The experiments were carried out on the Wistar rat model. An essential difference in the morphology of cells, both normal (erythrocytes) and cancerous, present in ascitic fluid taken from the non-treated group of rats and the groups treated with either dioxadet or cisplatin, has been observed. The results obtained can be interpreted as an indication of the antitumor performance of both cytostatics at the cellular level and as a demonstration of the higher efficacy of therapy with dioxadet as compared to that with cisplatin. Differences in cell morphology are suggested to be applied as quantitative markers of cell viability and cytostatic agent efficacy. The conclusions made are supported by a comparison with the results of recent experiments based on survival rates of laboratory animals treated with these agents.

High-speed in vitro intensity diffraction tomography

“…Quantifying these factors could be highly beneficial to immunology and pathophysiology applica-tions ,where longitudinal studies of parasite and bacterial interactions and induced morphological changes in cells carry critical information for understanding and mitigating infection [19,46]. Furthermore, quantifying volumetric morphological changes of cellular and subcellular information also has significant utility in oncology for both differentiating cancer types and evaluating their response to drug and therapy treatments [23][24][25].…”

High-speed in vitro intensity diffraction tomography

“…19,46 Furthermore, quantifying volumetric morphological changes of cellular and subcellular information also has significant utility in oncology, both for differentiating cancer types and evaluating their response to drug and therapy treatments. [23][24][25]…”

“…In ODT, the scattered field is directly measured from digitally recorded interferograms taken under different illumination angles (i.e., phase projection measurement). Several ODT approaches have been applied in biomedical studies for evaluating cell physiology, 17,18 pathophysiology and immunology, [19][20][21][22] oncology, [23][24][25] and micro-organism and intracellular particle tracking. 26,27 Notably, annular illumination is particularly effective in achieving high-quality 3-D RI recovery using a relatively small number of phase projection measurements.…”

Разработка Алгоритмов Сегментации В Голографической Микроскопии И Томографии Для Определения Морфологических Параметров Клеток

“…Большое количество флуорофоров, специфичных к различным типам белков, участвующих в важных биологических процессах, и быстрое развитие технологий повышения пространственного разрешения во флуоресцентной микроскопии [2] делают такие методы анализа живых клеток незаменимыми при изучении многих биологических процессов. Вместе с тем подходы, основанные на анализе морфологических характеристик клеток, попрежнему остаются актуальными и информативными как при использовании в клинической практике [3], так и в фундаментальных биологических исследованиях [4].…”

“…Измерения проводились на лабораторной установке голографического микроскопа (см. [4,7]), голографическом томографическом микроскопе 3D Cell Explorer (Nanolive) и конфокальном флуоресцентном микроскопе Leica TCS SP5.…”