Morphological, cytological, and molecular variations induced by gamma rays in ground-grown chrysanthemum ‘Pinkling’

Wu, Jianhui; Zhang, Jing; Lan, Feng; Fan, Weifang; Wen, Li

doi:10.1139/cjps-2019-0064

Cited by 11 publications

(11 citation statements)

References 23 publications

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“…The most effective primer was ISSR_21 and the worst was ISSR_62. These results indicate that the gamma-ray irradiation was the effective method to induce mutations in plants, as previously proved [2,8,14,23]. High PIC, EMR The genetic similarity based on Jaccards scores of the 'Earlibrite' strawberry mutant genotypes, ranged from 3% to 87.04%.…”

Section: Resultssupporting

confidence: 65%

“…In vitro gamma-ray mutagenesis is an alternative to increase genetic variation in strawberries. Gamma-ray irradiation causes higher mutation rates than other types of irradiation [2,7,8]. Gamma rays can generate free radicals in cells and tissues that cause biochemical, cytological, and physiological changes affecting plant growth and development [7,[9][10][11].…”

Section: Introductionmentioning

confidence: 99%

“…Even at low doses, gamma-ray irradiation induced nuclear mutation in plants [13]. Mutation in the plant can be detected molecularly using repetitive sequence marker, like ISSR as is done in chrysanthemums [2,8], citrus [14], strawberries [3] and grape [15]. This study aimed to identify the DNA polymorphism of 'Earlibrite' strawberry mutants using ISSR markers.…”

Section: Introductionmentioning

confidence: 99%

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Disclosing the Genetic Diversity of ‘Earlibrite’ Strawberry Mutant Induced by Gamma-ray Irradiation Using ISSR Markers

Arisah

Saptadi²,

Ashari³

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

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Strawberry plantlets of the ‘Earlibrite’ variety were irradiated with gamma rays to induce genetic variability for resistance to abiotic stress. This study aimed to identify the DNA polymorphism of ‘Earlibrite’ strawberry mutants using ISSR markers. For this purpose, a population of M1V0 strawberry plants was gamma-irradiated at 20 Gy. In addition, the irradiated plantlets were subcultured onto MS medium enriched with 0.13 M of 1-NAA and 2 M of 6-BAP. The genetic variation of the mutant was molecularly analyzed using the ISSR marker. Analysis was performed on M1V0 mutant plantlets. The results showed that 93.36% of 192 scoreable bands produced by the ISSR markers were polymorphic. An average of PIC values was 0.48 per primer with a range of 0.40 (ISSR_56) to 0.50 (ISSR_22, ISSR_55, ISSR_58, ISSR_60, ISSR_61, ISSR_62 and ISSR_L10). The primer MI ranged from 2.38 (ISSR_62) to 4.85 (ISSR_21). The genetic variation of mutant individuals ranged from 2.74% to 87.04% according to the Jaccard similarity index. Cluster analysis using ISSR markers grouped the ‘Earlibrite’ strawberry mutant genotypes into five distinct groups. It is concluded that 20 Gy gamma-ray induced mutation produces strawberry mutants and that ISSR was a valuable marker for mutant identification.

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Section: Resultssupporting

confidence: 65%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Disclosing the Genetic Diversity of ‘Earlibrite’ Strawberry Mutant Induced by Gamma-ray Irradiation Using ISSR Markers

Arisah

Saptadi²,

Ashari³

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

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“…Indeed, gamma-ray irradiation induces a sufficient frequency of mutations in plants. Using ISSR markers to further analyze the mutants may offer a useful molecular marker for the detection of mutants in the plant, which provides some valuable information for future breeding of ornamental plants ( Wu et al, 2020 ). Therefore, studying the physiological, cytological, and genetic effects of gamma-ray irradiation on tulip ( Tulipa gesneriana L.) is of great importance for breeding new tulip varieties and improving the quality of flowering plants.…”

Section: Introductionmentioning

confidence: 99%

Biological effects of gamma-ray radiation on tulip (Tulipa gesneriana L.)

Chen

Zhan

et al. 2022

PeerJ

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Tulip, being an important ornamental plant, generally requires lengthy and laborious procedures to develop new varieties using traditional breeding methods requires. But ionizing radiation potentially accelerates the breeding process of ornamental plant species. The biological effects of γ-ray irradiation on tulip, therefore, were investigated through establishing an irradiation-mediated mutation breeding protocol to accelerate its breeding process. ISSR-PCR molecular marker technique was further used to identify the mutants of phenotypic variation plants. This study showed that low irradiation doses (5 Gy) stimulated bulb germination to improve the survival rate of tulip, while high irradiation doses (20 to 100 Gy) significantly (P < 0.05) inhibited its seed germination and growth, and decreased the flowering rate, petal number, flower stem length and flower diameter. More than 40 Gy significantly (P < 0.05) decreased the total chlorophyll content and increased the malondialdehyde (MDA) content in tulips. Interestingly, three types of both stigma variations and flower pattern variations, and four types of flower colour variations were observed. With increasing the irradiation dose from 5 to 100 Gy, the anthocyanin and flavonoid contents continuously decreased. Scanning electron microscopy (SEM) analysis evidenced that high irradiation doses altered the micromorphology of leaf stomata. Microscopic observations of tulip root apical mitosis further showed the abnormal chromosomal division behaviour occurring at different mitotic phases under irradiation treatment (80 Gy). Increasing the irradiation dose from 20 to 100 Gy enhanced the micronucleus rate. Moreover, the suspected genetic variation in tulips was evaluated by inter-simple sequence repeat (ISSR) analysis, and the percentage of polymorphic bands was 68%. Finally, this study concludes that that 80 Gy may be an appropriate radiation does to better enhance the efficiency of mutagenic breeds in tulip plants. Using γ-ray irradiation, therefore, is expected to offer a theoretical basis for mutation breeding in tulips.

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“…In order to develop new plant species, gamma irradiation breeding is highly useful. Although, after applying gamma-ray irradiation, plant growth can be impeded, the number of leaves can reduce, the flowering time can be delayed, and the number of flowers can fall, the variation rate of flower characteristics can also greatly have boosted at the same time (Wu et al, 2020). Lower gamma irradiation doses have favorable and effective effects on morphological and physicochemical traits in different types of ornamental plants, while greater levels of irradiation generally have a negative impact.…”

Section: Introductionmentioning

confidence: 99%

Induction of mutagenesis on Chrysanthemums

Haspolat¹

2022

Ornam. Hortic.

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Crop genetic diversity has a significant role in improving new plants through breeding. The chrysanthemum contains the most mutant varieties, making mutation breeding one of the most widely utilized breeding procedures for ornamental plants. The goal of this research is to use gamma irradiation to induce genetic variation and mutation breeding to improve chrysanthemum features. In vitro bud explants of the white ‘Bacardi’ type were treated with gamma rays at 20 Gy on this scope. The explants were subcultured until M1V4 growing period occurred, and observations were made during blooming on this time. Variable flower head widths, distinction on plant heights and widths, numerous flower numbers, color and size variations of ray florets were among the mutagenic changes observed in plants and flowers. Ray florets varied in length, width, number of rows, and color. The mutation frequency of the population was estimated 1.1% and yellow-colored florets were developed whereas the control group remained white. The dendrogram was grouped into five groups with 1, 28, 31, and 41 mutants in each based on the plant height and width, plant stem height and width, number of shoots and flowers per plant, flower head width, ray florets’ number- height- color, number of leaves, leaf length and width, and weight of flowering stems. The yellow-colored mutants were located in the first, second, and fourth groups. The advantageous mutations could result in improving new varieties. Gamma radiation is an effective mutagen for creating new chrysanthemum types when applied to in vitro bud explants.

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Morphological, cytological, and molecular variations induced by gamma rays in ground-grown chrysanthemum ‘Pinkling’

Cited by 11 publications

References 23 publications

Disclosing the Genetic Diversity of ‘Earlibrite’ Strawberry Mutant Induced by Gamma-ray Irradiation Using ISSR Markers

Disclosing the Genetic Diversity of ‘Earlibrite’ Strawberry Mutant Induced by Gamma-ray Irradiation Using ISSR Markers

Biological effects of gamma-ray radiation on tulip (Tulipa gesneriana L.)

Induction of mutagenesis on Chrysanthemums

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