Morphological Evaluation of the Tissue Reaction to Subcutaneous Implantation of Decellularized Matrices

Сотниченко, А. С.; Накохов, Р. З.; Губарева, Е. А.; Куевда, Е. В.; Gumenyuk, I. S.

doi:10.1007/s10517-018-4334-0

Cited by 5 publications

(11 citation statements)

References 6 publications

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“…However, it must not be neglected that the immune reaction to heterotopic and orthotopic transplantation (OTX) may differ to some extent. [ 11,35 ]…”

Section: Discussionmentioning

confidence: 99%

“…[32] Removal of cellular components is realized by the sole application or a combination of physical, chemical, and enzymatic methods. [35,36] However, thorough decellularization often runs at the expense of ECM preservation as harsh processing methods and substances may cause ECM component reduction, disruption, and loss. [33,37] The balance between thoroughness of cell removal and preservation of ECM components depends on adjustable processing parameters, including type of detergent and time of exposure.…”

Section: Decellularization-based Scaffoldsmentioning

confidence: 99%

“…The Krasnodar group has recently examined both quantitative and qualitative non-invasive evaluation tools for verification of decellularization efficiency. [11,35,44,45] Accordingly: 1) quantitative determination of cell viability through electron paramagnetic resonance spectroscopy; and 2) monitoring free radical generation through chemiluminescence may serve as objectifiable decellularization quantification tools. Ultrasound imaging as a clinically proven safe examination method can likewise be employed for decellularized, as well as recellularized tissues through high-frequency acoustic microscopy prior to implanta-www.advancedsciencenews.com www.advtherap.com tion.…”

Section: Pre-implantation Evaluation Tools For Decellularization-base...mentioning

confidence: 99%

“…However, it must not be neglected that the immune reaction to heterotopic and orthotopic transplantation (OTX) may differ to some extent. [11,35] Besides patch properties, in vivo performance of tissueengineered constructs depends on multiple characteristics of the diaphragmatic defect itself. Complications of thereupon required patch repair occur in varying degrees according to pivotal variables -the 4 "S": implant site, affected side, defect size, and integration speed.…”

mentioning

confidence: 99%

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Tissue Engineering for the Diaphragm and its Various Therapeutic Possibilities – A Systematic Review

Boehm

Hillebrandt

Dziodzio

et al. 2022

Advanced Therapeutics

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Diaphragmatic impairments exhibit high morbidity as well as mortality while current treatment options remain unsatisfactory. Tissue engineering (TE) approaches have explored the generation of an optimal biocompatible scaffold for diaphragmatic repair through tissue decellularization or de novo construction, with or without the addition of cells. The authors conducted a systematic review on the current state of the art in diaphragmatic tissue engineering (DTE) and found 24 articles eligible for final synthesis. The included approaches studied decellularization-based graft generation (9) and de novo bioscaffold construction (9). Three studies focused on in vitro host-scaffold interaction with synthesized, recellularized grafts (2) and decellularized extracellular matrix scaffolds (1). Another three studies investigated evaluation tools for decellularization efficacy. Among all studies, recellularization is performed in both decellularization-based (4) and de novo generated scaffolds (4). De novo constructed biocomposites as well as decellularized and recellularized scaffolds induced pro-regenerative remodeling and recovery of diaphragmatic function in all examined animal models. Potential therapeutic applications comprise substance defects requiring patch repair, such as congenital diaphragmatic hernia, and functional diseases demanding an entire organ transplant, like muscular dystrophies or dysfunction after prolonged artificial respiration.

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“…However, it must not be neglected that the immune reaction to heterotopic and orthotopic transplantation (OTX) may differ to some extent. [ 11,35 ]…”

Section: Discussionmentioning

confidence: 99%

Section: Decellularization-based Scaffoldsmentioning

confidence: 99%

Section: Pre-implantation Evaluation Tools For Decellularization-base...mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Tissue Engineering for the Diaphragm and its Various Therapeutic Possibilities – A Systematic Review

Boehm

Hillebrandt

Dziodzio

et al. 2022

Advanced Therapeutics

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show abstract

“…Once deceullularisation has removed cellular material, the remaining ECM retains the native 3D microstructure, molecular composition and growth factors that support skeletal muscle regeneration [25]. Decellularization protocols vary between studies but commonly include the use of detergents and enzymes, such as DNase and Trypsin; the effectiveness of the decellularization protocol is central to minimising scaffold immunogenicity [27][28][29][30][31].…”

Section: Decellularised Scaffoldsmentioning

confidence: 99%

Regenerative medicine for skeletal muscle loss: a review of current tissue engineering approaches

Langridge

Butler

2021

J Mater Sci: Mater Med

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Skeletal muscle is capable of regeneration following minor damage, more significant volumetric muscle loss (VML) however results in permanent functional impairment. Current multimodal treatment methodologies yield variable functional recovery, with reconstructive surgical approaches restricted by limited donor tissue and significant donor morbidity. Tissue-engineered skeletal muscle constructs promise the potential to revolutionise the treatment of VML through the regeneration of functional skeletal muscle. Herein, we review the current status of tissue engineering approaches to VML; firstly the design of biocompatible tissue scaffolds, including recent developments with electroconductive materials. Secondly, we review the progenitor cell populations used to seed scaffolds and their relative merits. Thirdly we review in vitro methods of scaffold functional maturation including the use of three-dimensional bioprinting and bioreactors. Finally, we discuss the technical, regulatory and ethical barriers to clinical translation of this technology. Despite significant advances in areas, such as electroactive scaffolds and three-dimensional bioprinting, along with several promising in vivo studies, there remain multiple technical hurdles before translation into clinically impactful therapies can be achieved. Novel strategies for graft vascularisation, and in vitro functional maturation will be of particular importance in order to develop tissue-engineered constructs capable of significant clinical impact.

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Extracellular matrix scaffold-immune microenvironment modulates tissue regeneration

Zhang

Liu

et al. 2022

Composites Part B: Engineering

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Morphological Evaluation of the Tissue Reaction to Subcutaneous Implantation of Decellularized Matrices

Cited by 5 publications

References 6 publications

Tissue Engineering for the Diaphragm and its Various Therapeutic Possibilities – A Systematic Review

Tissue Engineering for the Diaphragm and its Various Therapeutic Possibilities – A Systematic Review

Regenerative medicine for skeletal muscle loss: a review of current tissue engineering approaches

Extracellular matrix scaffold-immune microenvironment modulates tissue regeneration

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