Morphological findings in the liver of diabetic rats after intraportal transplantation of neonatal isologous pancreatic islets

Cossel, L; Wohlrab, F; Blech, W; Hahn, Hans-Jürgen

doi:10.1007/bf02899389

Cited by 13 publications

(6 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since this dose of pentobarbital causes anesthesia in a rat, these data suggest that the intrahepatic pacemaker and system for propagation of the signal between islets are relatively resistant to suppression by anesthetic doses of barbiturate and therefore are analogous to the sinoatrial node and Purkinje tissue present in the heart (18). Using synaptophysin labeling, islet innervation was clearly demonstrable in 12 out of 12 200-d Tx livers but not in any of the early Tx livers, consistent with previous studies (12)(13)(14). Innervation was confirmed by the presence of synaptophysin staining nerve endings penetrating the transplanted islet as indicated in Fig.…”

Section: A 30supporting

confidence: 91%

“…Since islets at this time are still intraportal where they may be aggregated ( 12), this single case most likely represented coordinate pulsatile insulin secretion from an aggregate of islets as occurs in periperfused islets (30,31). However, by 7 d after transplantation the islets had become topographically dispersed within the hepatic parenchyma (12), and at that time there were no high frequency coordinate secretory bursts present, although they became reestablished between 30 and 200 d, which coincides with the time period described for the establishment of islet reinnervation (12)(13)(14). Taken together these data are consistent with the hypothesis that coordinate high frequency insulin secretory bursts by islets dispersed within an organ are mediated by interislet innervation, which allows for transmission of a synchronizing signal or a wave of depolarization throughout the islet population (27).…”

Section: Discussionmentioning

confidence: 78%

“…If coordinate insulin secretion is mediated by the intrapancreatic neural network (11), it might be expected that the dispersal of transplanted islets throughout the liver would result in loss of pulsatile insulin secretion. However, since intrahepatic transplanted islets are reinnervated (12)(13)(14), a process that has been identified in occasional islets -4 wk after transplantation (12) and has become clearly established by 14 wk (14), it is plausible that coordinate pulsatile insulin secretion is restored in chronically transplanted islets.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Coordinate pulsatile insulin secretion by chronic intraportally transplanted islets in the isolated perfused rat liver.

et al. 1994

View full text Add to dashboard Cite

In the present studies we sought to address the following questions: do chronically transplanted intrahepatic islets (IHI-Tx) secrete insulin in a coordinate pulsatile manner, and, if so, is reestablishment of this coordinate pulsatility a function of time after transplantation? We studied isolated perfused livers at 10 mM glucose from 27 rats rendered diabetic with streptozotocin and then transplanted with -2 x 103 islets, 2 (n = 5), 7 (n = 5), 30 (n = 5), and 200 (n = 12) d after transplantation. (-200 d) 111-Tx secrete insulin in a coordinate pulsatile manner and that establishment of coordinate pulsatile insulin secretion by IHI-Tx is accompanied by increased total insulin secretion and is associated with islet reinnervation. (J. Clin. Invest. 1994. 94: 219-227.)

show abstract

Section: A 30supporting

confidence: 91%

Section: Discussionmentioning

confidence: 78%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Coordinate pulsatile insulin secretion by chronic intraportally transplanted islets in the isolated perfused rat liver.

et al. 1994

View full text Add to dashboard Cite

show abstract

“…This is, however, important because one of the arguments for implanting islets intraportally into the liver is that nutrients and other substances in the portal blood could directly affect islet function. In long-term intra-hepatic islet grafts there is often a disruption of the normal islet structure, with strands of endocrine cells sometimes entering the liver lobuli [94,95], which probably improves blood supply.…”

Section: Vasculature Of Transplanted Isletsmentioning

confidence: 99%

Graft vascular function after transplantation of pancreatic islets

2002

View full text Add to dashboard Cite

Endogenous pancreatic islets have a dense glomerular-like angioarchitecture, which ensures an optimal delivery of oxygen and nutrients to the islet cells, provides signals from other cells in the body and disposes secreted hormones. Transplantation of isolated islets means that their vascular connection is interrupted. The islet grafts therefore depend upon endothelial cells and microvessels originating in the implantation organ for derivation of a new vascular system. A re-establishment of islet blood-flow occurs within 7-14 days after transplantation, mainly through vascular sprouting. The newly formed blood vessels acquire the morphological characteristics of those in endogenous islets. In intraportally transplanted islets to the liver, the islets become revascularized almost exclusively from tributaries to the hepatic artery. Exocrine contamination of the transplanted islets could hamper the revascularization process, whereas neither cryopreservation nor immunosuppressive drugs like cyclosporin, prednisolon and RS-61443 have any essential effects on the angiogenesis. Investigators have noticed improvements in islet graft survival and function by means of basic fibroblast growth factor (bFGF), acidic FGF and endothelial cell growth factor exposure of the grafts. The functional properties of transplanted islets are largely unknown, but evidence from experimental islet transplantation suggests that both the blood perfusion and the tissue oxygen tension of the grafted islets are chronically decreased, indicating an insufficient vascular system. In order to achieve optimal condition for survival and function of transplanted beta cells, it is important to ascertain whether impairments in vascular function are present also after clinical islet transplantations as well.

show abstract

“…The presence of unmyelinated nerve fibers in islets or fetal pancreases transplanted into the liver (3,8), spleen (9), or anterior eye chamber (10) has been noted, but systematic investigations of the presence of different types of nerves within the grafts are lacking. The aim of this study therefore was to evaluate, with immunocytochemical and enzyme histochemical techniques, the development and extent of the reinnervation of transplanted syngeneic pancreatic islets implanted into the renal subcapsular space in mice.…”

mentioning

confidence: 97%

Reinnervation of Syngeneic Mouse Pancreatic Islets Transplanted Into Renal Subcapsular Space

et al. 1992

View full text Add to dashboard Cite

A syngeneic transplantation of 150 islets into the subcapsular renal space was performed on normoglycemic or alloxan-induced diabetic male C57BL/6 mice. Six, 8, 14, or 20-21 wk after transplantation, the graft-bearing kidney was removed and processed for microscopical examinations with indirect immunofluorescence for neuropeptides and tyrosine hydroxylase, and with acetylcholinesterase staining to visualize nerve fibers within the graft. Six weeks after implantation, only a few scattered nerve fibers were observed within the grafts. A progressive increase in the number of nerves was observed until 14 wk after transplantation, after which, a stable level was reached. Alloxan-induced diabetic mice showed quantitatively and qualitatively similar reinnervation to normoglycemic mice 20 wk after transplantation. The findings demonstrate the presence of sympathetic nerve fibers (containing tyrosine hydroxylase and neuropeptide Y), mainly accompanying ingrowing blood vessels; parasympathetic nerve fibers (containing acetylcholinesterase and vasoactive intestinal peptide), possibly reaching the graft from the adjacent renal capsule; and afferent nerve fibers (containing substance P and calcitonin gene-related peptide), which were less numerous. The data suggest that transplanted islets become reinnervated by ingrowth of nerve fibers from the implantation organ and that several types of nerves are present.

show abstract

Morphological findings in the liver of diabetic rats after intraportal transplantation of neonatal isologous pancreatic islets

Cited by 13 publications

References 18 publications

Coordinate pulsatile insulin secretion by chronic intraportally transplanted islets in the isolated perfused rat liver.

Coordinate pulsatile insulin secretion by chronic intraportally transplanted islets in the isolated perfused rat liver.

Graft vascular function after transplantation of pancreatic islets

Reinnervation of Syngeneic Mouse Pancreatic Islets Transplanted Into Renal Subcapsular Space

Contact Info

Product

Resources

About