Morphological, functional, compositional and transcriptional constraints shape the distinct venom profiles of the assassin bug Sycanus croceovittatus

Wu, Chaoyan; Li, Lu; Wang, Yuqin; Wei, Shujun; Zhu, Jiaying

doi:10.1016/j.ijbiomac.2023.126162

Cited by 3 publications

(1 citation statement)

References 65 publications

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“…Notably, while most of these proteins could be functionally categorized and are commonly observed in the venoms of parasitoids within the Braconidae family and other investigated families [ 54 , 55 ], DDOST and those venom proteins with unknown functions stood out as an exception. DDOST was recently recognized as a venom component in the venom of the assassin bug Sycanus croceovittatus [ 56 ]. Those venom proteins in H. hebetor that lack known functions may represent recently evolved components that warrant detailed functional elucidation.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis Reveals the Venom Genes of the Ectoparasitoid Habrobracon hebetor (Hymenoptera: Braconidae)

Yang,

Lu,

Wang

et al. 2024

Insects

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The ectoparasitoid Habrobracon hebetor (Hymenoptera: Braconidae) exhibits a broad parasitic capability towards various lepidopteran pests, with venom serving as a crucial virulent factor ensuring successful parasitization and subsequent host mortality. Analyzing the constituents of its venom is essential for elucidating the mechanisms underlying efficient host killing by this parasitoid and for exploring potentially functional venom proteins. Through a transcriptomic analysis, a total of 34 venom proteins were identified within the venom of H. hebetor, encompassing known components such as serine protease, metalloproteinase, esterase, and serine protease inhibitors commonly present in parasitoid venoms. Unique components like paralytic protein and ion transport peptide-like were identified, possibly specific to certain parasitoids, along with novel proteins with uncharacterized functions. Spatial gene expression profiling of the identified venom proteins using transcriptomic data, corroborated by quantitative PCR validation for 13 randomly selected proteins, revealed abundant expression levels in the venom apparatus, affirming them as genuine venom components. Notably, the paralytic protein exhibited prominent expression, with the highest FPKM (fragments per kilobase of transcript per million fragments mapped) value of 24,704.87 in the venom apparatus, indicative of its significant role in successful parasitism by H. hebetor. The identification of these venom proteins establishes a foundation for the further exploration of bioactive agents for pest management strategies.

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Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis Reveals the Venom Genes of the Ectoparasitoid Habrobracon hebetor (Hymenoptera: Braconidae)

Yang,

Lu,

Wang

et al. 2024

Insects

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Insecticidal activities of three recombinant venom proteins of the predatory stink bug, Arma custos

Wang,

Li,

Yang

et al. 2024

Pest Management Science

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BACKGROUNDWidespread resistance of insect pests to insecticides and transgenic crops in the field is a significant challenge for sustainable agriculture, and calls for the development of novel alternative strategies to control insect pests. One potential resource for the discovery of novel insecticidal molecules is natural toxins, particularly those derived from the venoms of insect predators.RESULTSIn this study, we identified three insecticidal proteinaceous toxins from the venom glands (VGs) of the predatory stink bug, Arma custos (Hemiptera: Asopinae). Transcriptomic analysis of A. custos VGs revealed 151 potentially secreted VG‐rich venom proteins. Three VG‐rich venom proteins (designated AcVP1 ~ 3) were produced by overexpression in Escherichia coli. Injection of the recombinant proteins into tobacco cutworm (Spodoptera litura) larvae showed that all of the three recombinant proteins caused paralysis, liquefaction and death. Injection of recombinant proteins into rice brown planthopper (Nilaparvata lugens) nymphs showed higher insecticidal activities, among which a trypsin (AcVP2) caused 100% mortality postinjection at 1.27 pmol mg−1 body weight.CONCLUSIONA natural toolkit for the discovery of insecticidal toxins from predatory insects has been revealed by the present study. © 2024 Society of Chemical Industry.

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Venom exaptation and adaptation during the trophic switch to blood-feeding by kissing bugs

Zdenek,

Cardoso,

Robinson

et al. 2024

iScience

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Morphological, functional, compositional and transcriptional constraints shape the distinct venom profiles of the assassin bug Sycanus croceovittatus

Cited by 3 publications

References 65 publications

Transcriptome Analysis Reveals the Venom Genes of the Ectoparasitoid Habrobracon hebetor (Hymenoptera: Braconidae)

Transcriptome Analysis Reveals the Venom Genes of the Ectoparasitoid Habrobracon hebetor (Hymenoptera: Braconidae)

Insecticidal activities of three recombinant venom proteins of the predatory stink bug, Arma custos

Venom exaptation and adaptation during the trophic switch to blood-feeding by kissing bugs

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