Morphological, histological, and molecular description of Myxobolus ompok n. sp. (Myxosporea: Myxobolidae), a kidney myxozoan from Pabdah catfish Ompok pabda (Hamilton, 1822) (Siluriformes: Siluridae) in India

Abstract

In a parasitological survey of freshwater fishes near Meerut, Uttar Pradesh, India, myxozoan infections in Pabdah catfish Ompok pabda Ham. (Siluriformes: Siluridae) were found. Round plasmodia and scattered spores of Myxobolus ompok n. sp. were found in the kidney of the host. Plasmodia measuring 150-200 μm were located in the renal interstitium. Spores of Myxobolus ompok n. sp. were elongated pyriform, 13.6-14.4 (14.8 ± 0.42) μm long, 5.6-6.4 (6.5 ± 0.33) μm wide, and 5.2-6.4 (5.9 ± 0.43) μm thick with two equal polar capsules measuring 8.0-8.5 (8.2 ± 0.2) μm in length and 1.5-2.4 (1.8 ± 0.33) in width having six filamental turns. Both the morphology and DNA analysis of the 18S rRNA gene revealed that Myxobolus ompok n. sp. is distinct from previously described species of Myxobolus and shares no significant similarity with any other Myxobolus deposited in the GenBank database. Phylogenetic analysis inferred that this species showed the closest similarity to Myxobolus miyarii (KT001495). This is the first record of any Myxobolus sp. from O. pabda in India.

The occurrence of known Myxobolus and Thelohanellus species (Myxozoa, Myxosporea) from Indian major carps with the description of Myxobolus bandyopadhyayi n. sp. in West Bengal

“…Unfortunately, the majority of the Indian myxosporean species have been poorly described: they are characterised only by morphometrical measurements and by the shape of spores and polar capsules, and less information is provided about the location of plasmodia in infected tissues. The validity of several Indian myxosporean species has recently been supported by molecular biological data ( Székely et al, 2015 ; Chaudhary et al, 2018 , 2019 ; Gupta et al, 2018 ). Presumably, more in-depth investigations supplemented with sequence data might prove several species to be synonyms.…”

“…However, the description of only a part of these species has been complemented with molecular data, therefore it might be possible that several synonymous species exist among them. Gupta et al (2021) mention 40 species of myxobolids with sequence data based on only a limited number of research studies ( Singh and Kaur, 2012 ; Kaur, 2014 ; Rajesh et al, 2014 ; Abraham et al, 2015 ; Székely et al, 2015 ; Chaudhary et al, 2018 , 2019 ; Gupta et al, 2018 ). Myxozoan samples were obtained from the genera Labeo , Gibelion and Cirrhinus collected from West Bengal fish farms and local fish markets which showed signs of myxosporean infections.…”

Molecular Characterization of Two Myxosporean Species, Henneguya namae Haldar et al. 1983 and Myxobolus sophorae Jayasri, 1982 (Myxosporea: Myxobolidae)

“…Among myxozoan, Henneguya Thélohan 1892 and Myxobolus Bütschli 1882 are the species rich genera and reported worldwide (Eiras 2002, Lom and Dyková 2006, Eiras and Adriano 2012, Liu et al 2018. However, from the family Myxobolidae, more than 150 species have been described in India and most of the Henneguya and Myxobolus species were recorded from the freshwater environment in India (Kalavati and Nandi 2007, Kaur et al 2015, Gupta and Kaur 2017, Chaudhary et al 2018, Ahmed et al 2019, Chaudhary et al 2019. Though, in comparing to the large number of species described, the molecular data is available only to a small percentage of them.…”

Morphological, histopathological and molecular characterization of Myxobolus szekelyianus n. sp. (Cnidaria: Myxosporea: Myxobolidae) causing acute gill disease in Schizothorax esocinus (Heckel, 1838) from River Jhelum of Kashmir Himalayan region, India

“…Andree et al (1999) also studied small subunit ribosomal DNA sequences of some Myxobolus species. Further, studies based on molecular data have also been included in some recent Indian research reports on myxozoan parasites (Dar et al, 2017a(Dar et al, , 2017bGupta & Kaur, 2017aChaudhary et al, 2018).…”