Morphological, molecular characterization, plant pathogenicity and biocontrol of Cladosporium complex groups associated with faba beans

El‐Dawy, Eman G. A.; Gherbawy, Youssuf A.; Hussein, Mohamed A.

doi:10.1038/s41598-021-93123-w

Cited by 28 publications

(16 citation statements)

References 26 publications

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“…The slide was then stained with methylene blue and observed and photographed under an optical microscope (Nikon DS-Ril-U2, Tokyo, Japan). The morphological results were recorded and compared with knowledge from published literature for preliminary characterization, which was further investigated and verified by the DNA sequence shown below [ 48 , 49 , 50 , 51 , 52 , 53 , 54 ].…”

Section: Methodsmentioning

confidence: 99%

Endophytic Fungi Isolated from Ageratina adenophora Exhibits Potential Antimicrobial Activity against Multidrug-Resistant Staphylococcus aureus

Wen

Okyere

Wang

et al. 2023

Plants

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Multidrug-resistant bacteria such as Staphylococcus aureus (MRSA) cause infections that are difficult to treat globally, even with current available antibiotics. Therefore, there is an urgent need to search for novel antibiotics to tackle this problem. Endophytes are a potential source of novel bioactive compounds; however, the harnessing of novel pharmacological compounds from endophytes is infinite. Therefore, this study was designed to identify endophytic fungi (from Ageratina adenophora) with antibacterial activity against multidrug-resistant bacteria. Using fungal morphology and ITS-rDNA, endophytic fungi with antibacterial activities were isolated from A. adenophora. The results of the ITS rDNA sequence analysis showed that a total of 124 morphotype strains were identified. In addition, Species richness (S, 52), Margalef index (D/, 7.3337), Shannon–Wiener index (H/,3.6745), and Simpson’s diversity index (D, 0.9304) showed that A. adenophora have abundant endophytic fungi resources. Furthermore, the results of the agar well diffusion showed that the Penicillium sclerotigenum, Diaporthe kochmanii, and Pestalotiopsis trachycarpicola endophytic fungi’s ethyl acetate extracts showed moderate antibacterial and bactericidal activities, against methicillin-resistant Staphylococcus aureus (MRSA) SMU3194, with a MIC of 0.5–1 mg/mL and a MBC of 1–2 mg/mL. In summary, A. adenophora contains endophytic fungi resources that can be pharmacologically utilized, especially as antibacterial drugs.

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Section: Methodsmentioning

confidence: 99%

Endophytic Fungi Isolated from Ageratina adenophora Exhibits Potential Antimicrobial Activity against Multidrug-Resistant Staphylococcus aureus

Wen

Okyere

Wang

et al. 2023

Plants

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“…El-Dawy et al 15 technique was used to perform the pathogenicity test. One-third portion of root system of 10–13 days old seedlings was clipped off from distal end and dipped for 5 min in conidial suspension prepared from the spores of purified fungal culture isolated from root rot affected pea plants.…”

Section: Methodsmentioning

confidence: 99%

Effect of the combination of biological, chemical control and agronomic technique in integrated management pea root rot and its productivity

Nazir

Badri²,

Bhat³

et al. 2022

Sci Rep

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Root rot of pea caused by Fusarium spp. is one of the important diseases of pea (Pisum sativum L.). The causal fungus of the disease isolated from naturally infected pea plants was identified as Fusarium solani f. sp. pisi (Jones). Evaluation of four bio agents and nine fungicides was done in vitro against Fusarium solani. Trichoderma harzianum was the most effective bio agent in inhibiting the mycelial growth of F. solani by (82.62%). Carbendazim 50 WP was the most effective fungicide in inhibiting the mycelial growth of F. solani by (91.06%). Carbendazim at the rate of 0.1% and T. harzianum at concentration of 109 cfu when used as seed treatment under field conditions were evaluated along with three planting techniques v.i.z, raised beds, ridges and flat beds. It was found that Carbendazim at the rate of 0.1% when given as seed treatment in raised beds exhibited the lowest disease incidence (10.97%), intensity (2.89%) and the maximum pod yield (89.63 q ha−1) as compared to control.

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“…These descriptions are all in line with the morphological characteristics of Cladosporium sp. (El-Dawy et al 2021;Ellis 2021b).…”

Section: Morphological Identification Of Fungal Isolate From Sweet Tapaimentioning

confidence: 99%

Isolation and characterization of indigenous amylolytic enzyme-producing Aspergillus sp. from sweet-flavored tapai

ANASTASIA

KODRAT²,

VICTOR³

et al. 2022

Biodiversitas

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Abstract. Anastasia L, Kodrat EA, Victor H, Sanjaya A, Pinontoan R. 2022. Isolation and characterization of indigenous amylolytic enzyme-producing Aspergillus sp. from sweet-flavored tapai. Biodiversitas 23: 5580-5586. Aspergillus sp. are able to produce abundant amylolytic enzymes that may have a crucial role in producing the sweet flavor of tapai. This study was aimed to evaluate the amylolytic activity of Aspergillus sp. isolated from a widely known sweet tapai from Jember, East Java, Indonesia. The fungi isolated from sweet tapai were identified under notable morphological parameters, and its amylolytic activities were evaluated by utilizing various starch-based media, such as potato peel, potato dextrose broth, cassava, and maize. Optimum pH and temperature of partially purified amylolytic enzymes were evaluated using DNS assay. The result of this study suggested that TM3 fungal isolate isolated from sweet tapai belonged to Aspergillus sp. section Nigri. Aspergillus sp. TM3 grown in the potato peel medium displayed the highest amylolytic activity, followed by those grown in maize, potato dextrose broth, and cassava media. Further characterization of partially purified amylolytic enzymes from the isolate showed that optimal pH for the amylolytic activity was at pH 4.59 while the optimal temperature was around 54°C. Furthermore, analysis by thin-layer chromatography revealed that the amylolytic enzymes starch hydrolysis yielded dextrose as the main product and oligosaccharides as the side product, which indicated that Aspergillus sp. TM3 may have both alpha-amylase and glucoamylase involved in its amylolytic activity. This study explored the role of Aspergillus sp. TM3 in producing the sweet flavor of tapai.

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Morphological, molecular characterization, plant pathogenicity and biocontrol of Cladosporium complex groups associated with faba beans

Cited by 28 publications

References 26 publications

Endophytic Fungi Isolated from Ageratina adenophora Exhibits Potential Antimicrobial Activity against Multidrug-Resistant Staphylococcus aureus

Endophytic Fungi Isolated from Ageratina adenophora Exhibits Potential Antimicrobial Activity against Multidrug-Resistant Staphylococcus aureus

Effect of the combination of biological, chemical control and agronomic technique in integrated management pea root rot and its productivity

Isolation and characterization of indigenous amylolytic enzyme-producing Aspergillus sp. from sweet-flavored tapai

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