Morphological, physiological and genomic comparisons between diploids and induced tetraploids in Anemone sylvestris L.

Zahumenická, Pavla; Lojka, Bohdan; Šedivá, J.; Žiarovská, Jana; Ros-Santaella, José Luis; Martínez-Fernández, Domingo; Russo, Daniela; Milella, Luigi

doi:10.1007/s11240-017-1331-3

Cited by 25 publications

(14 citation statements)

References 46 publications

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“…Leaf samples from each R and S biotypes were analysed by flow cytometry, using a Partec PAS flow cytometer (Partec GmbH, Münster, Germany) equipped with a high‐pressure mercury arc lamp, as described by Zahumenická et al 29 . Chromosome counting was performed using α‐bromonaphthalene pretreatment 30 …”

Section: Methodsmentioning

confidence: 99%

Enhanced metabolism and target gene overexpression confer resistance against acetolactate synthase‐inhibiting herbicides in Bromus sterilis

Sen

Hamouzová

Mikulka

et al. 2021

Pest Management Science

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BACKGROUND Intensive application of acetolactate synthase (ALS)‐inhibiting herbicides has resulted in herbicide‐resistance in many weeds, including Bromus sterilis. The present study was conducted to identify the mechanisms conferring resistance to ALS‐inhibiting herbicides in a Bromus sterilis biotype. RESULTS Dose–response studies revealed the resistant biotype to be 288 times less sensitive to pyroxsulam than the susceptible biotype. Furthermore, experiment with a single‐dose, proved this biotype was also cross‐resistant to propoxycarbazone, iodosulfuron plus mesosulfuron and sulfosulfuron. Prior treatment with malathion, a known inhibitor of cytochrome P450s, reduced the level of resistance to pyroxsulam. No mutations were detected from the partial ALS gene sequencing. Flow cytometry and chromosome counting rejected ploidy level variation between the susceptible and resistant biotypes. Relative copy number variation ruled out gene amplification. Quantitative real‐time polymerase chain reaction (PCR) detected a significant difference in ALS gene expression between the susceptible and resistant biotypes. CONCLUSIONS Target gene overexpression and enhanced metabolism by cytochrome P450s are likely mechanisms of resistance to pyroxsulam in Bromus sterilis. The current findings highlight the need to monitor additional brome populations for herbicide resistance in Europe and endorse the need for alternate herbicides in integrated weed management to delay the possible evolution of herbicide resistance in these species. © 2020 Society of Chemical Industry

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Section: Methodsmentioning

confidence: 99%

Enhanced metabolism and target gene overexpression confer resistance against acetolactate synthase‐inhibiting herbicides in Bromus sterilis

Sen

Hamouzová

Mikulka

et al. 2021

Pest Management Science

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“…Borna et al (2017) studied the applicability of iPBS markers for assessment of molecular variations and genetic relationships between 89 genotypes of Leonurus cardiaca L. In this study, 7 iPBS primers were used to amplify a total of 191 fragments ranging from 180 bp to 4,000 bp. Záhumenická et al (2018) used iPBS markers for investigation of genomic stability in Amenome sylvestris diploid and tetraploid plants. All these studies demonstrate that iPBS analysis is quick, reliable and that this approach produces sufficient polymorphisms for large-scale DNA fingerprinting purposes.…”

Section: Resultsmentioning

confidence: 99%

Habitat-related specifity of iPBS fingerprint in European populations of Hedera helix L.

et al. 2019

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Žiarovská, J., Ražná, K., Fernandéz, E.C., Bošeľová, D., Kyseľ, M., 2019. Habitat-related specifity of iPBS fingerprint in European populations of Hedera helix L. Folia Oecologica, 46: 30-36.Inter primer binding site (iPBS) polymorphism was investigated for common ivy (Hedera helix, L.) to obtain the knowledge on genetic diversity in this species. Actually, a very limited information exists about application of DNA markers in Hedera helix. Natural and planted European populations of ivy were analysed using an iPBS marker 5´ACCTGGCGTGCCA3´ with a total number of 238 fragments generated. Of these, 86% were polymorphic. There were determined certain attributes of this marker such as the diversity index (DI) and polymorphism information content (PIC). The value of the diversity index was 0.79 and the polymorphic information index was 0.78. The proportion of polymorphisms of the individual amplified loci ranged from 0.32% to 6.98%. Cluster analysis was performed to determine the relationships among the European ivy populations where the distribution in the dendrogram under the habitat specifity was found for the used iPBS marker. We concluded that iPBS was very efficient in analysing the genetic diversity in Hedera helix, L. and that this marker can serve as a suitable tool to find genomically specific fingerprints relevant to the factors influencing the distribution of genetic variation.

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“…Several researchers, such as Al-Najm et al (2016); Gedik et al (2017) and Coutinho et al (2018), have reported the high efficiency of the iPBS markers in identifying the high genetic diversity among plants. Studies in literature are newly emerging on the usage of this technique produced with plant tissue culture (Gozukirmizi 2014; Zahumenická et al 2018). Genetic stability was also determined with iPBS as the second method black carrot plantlets were investigated with seven iPBS primers.…”

Section: Resultsmentioning

confidence: 99%

Plant regeneration from hypocotyls of black carrot via direct somatic embryogenesis and determination of its genetic stability by RAPD and iPBS methods

Çetin¹

2018

IJGPB

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A study was carried out to investigate plant regeneration from hypocotyls of black carrot (Daucus carota spp. sativus), an industrially and medicinally important plant via somatic embryogenesis, and the genetic stability of obtained plantlets with the random amplified polymorphic DNA (RAPD) and inter primer binding sites (iPBS) methods. Hypocotyl explants isolated from in vitro germinated seeds were incubated initially at Murashige and Skoog (MS) medium containing 0.5 mg/L or 1 mg/L 2,4-D for 2 weeks and then at hormone-free MS medium in order to obtain somatic embryos, which were observed after 15 days on the explants. Performed molecular analyses showed that all bands obtained from plantlets through micropropagation were monomorphic. This protocol where black carrot was determined to be clonally reproduced through somatic embryogenesis may lead the way for further research regarding germplasm conservation and breeding studies.

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Morphological, physiological and genomic comparisons between diploids and induced tetraploids in Anemone sylvestris L.

Cited by 25 publications

References 46 publications

Enhanced metabolism and target gene overexpression confer resistance against acetolactate synthase‐inhibiting herbicides in Bromus sterilis

Enhanced metabolism and target gene overexpression confer resistance against acetolactate synthase‐inhibiting herbicides in Bromus sterilis

Habitat-related specifity of iPBS fingerprint in European populations of Hedera helix L.

Plant regeneration from hypocotyls of black carrot via direct somatic embryogenesis and determination of its genetic stability by RAPD and iPBS methods

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