Morphological Processes of Foot Process Effacement in Puromycin Aminonucleoside Nephrosis Revealed by FIB/SEM Tomography

Ichimura, Koichiro; Miyaki, Takayoshi; Kawasaki, Yuto; Kinoshita, Mui; Kakuta, Soichiro; Sakai, Tatsuo

doi:10.1681/asn.2018020139

Cited by 31 publications

(30 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Recently, we revealed the three-dimensional (3D) ultrastructural architecture of podocytes using focused ion beam/ scanning electron microscopy (FIB/SEM) tomography in combination with a reconstruction technique (Ichimura et al 2015(Ichimura et al , 2019) (see Fig. S1 for further details of this technique).…”

Section: R Bmentioning

confidence: 99%

Three-dimensional architecture of pericardial nephrocytes in Drosophila melanogaster revealed by FIB/SEM tomography

et al. 2019

Self Cite

View full text Add to dashboard Cite

Nephrocytes are similar in structure to podocytes and play a role in the isolation of toxic substances from hemolymph in insects. Drosophila melanogaster nephrocytes have recently been used to study podocyte function and disease. However, the threedimensional ultrastructure of nephrocytes is not clearly understood because their surrounding basement membrane makes it difficult to observe using conventional scanning electron microscopy. We reconstructed the three-dimensional ultrastructure of Drosophila pericardial nephrocytes using serial focused-ion beam/scanning electron microscopy (FIB/SEM) images. The basal surfaces were occupied by foot processes and slit-like spaces between them. The slit-like spaces corresponded to the podocyte filtration slits and were formed by longitudinal infolding/invagination of the basal plasma membrane. The basal surface between the slit-like spaces became the foot processes, which ran almost linearly, and had a Bwashboard-like^appearance. Both ends of the foot processes were usually anastomosed to neighboring foot processes and thus free ends were rarely observed. We demonstrated that FIB/SEM is a powerful tool to better understand the three-dimensional architecture of nephrocytes.

show abstract

Section: R Bmentioning

confidence: 99%

Three-dimensional architecture of pericardial nephrocytes in Drosophila melanogaster revealed by FIB/SEM tomography

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…For instance, we can show that podocytes frequently form cytoplasmic fragmentation (Fig. 7c′) and autocellular tight junctions during the developmental period of the foot process effacement in puromycin aminonucleoside (PAN) nephrosis (Ichimura et al 2019).…”

Section: Fib-sem Tomographymentioning

confidence: 99%

“…This is not possible with conventional FE-SEM. Our research group analyzed the 3D ultrastructure of podocytes in health, disease and development by utilizing FE-SEM and FIB-SEM tomography Ichimura et al 2019;Ichimura et al 2015;Ichimura and Sakai 2017;Kawasaki et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Three-dimensional imaging of podocyte ultrastructure using FE-SEM and FIB-SEM tomography

et al. 2019

Self Cite

View full text Add to dashboard Cite

Podocytes are specialized epithelial cells used for glomerular filtration in the kidney. They can be divided into the cell body, primary process and foot process. Here, we describe two useful methods for the three-dimensional(3D) visualization of these subcellular compartments in rodent podocytes. The first method, field-emission scanning electron microscopy (FE-SEM) with conductive staining, is used to visualize the luminal surface of numerous podocytes simultaneously. The second method, focusedion beam SEM (FIB-SEM) tomography, allows the user to obtain serial images from different depths of field, or Z-stacks, of the glomerulus. This allows for the 3D reconstruction of podocyte ultrastructure, which can be viewed from all angles, from a single image set. This is not possible with conventional FE-SEM. The different advantages and disadvantages of FE-SEM and FIB-SEM tomography compensate for the weaknesses of the other. The combination renders a powerful approach for the 3D analysis of podocyte ultrastructure. As a result, we were able to identify a new subcellular compartment of podocytes, "ridge-like prominences" (RLPs).

show abstract

“…Due to the interconnection by the SD (indicated by the continuous black line at the interface of the green and purple FPs), this structure represents a completely interconnected homogenous cell layer that covers the entire tuft. A and B reproduced from Ichimura et al (2019) with permission…”

Section: F I G U R Ementioning

confidence: 99%

The Inability of Podocytes to Proliferate: Cause, Consequences, and Origin

Kriz

2019

The Anatomical Record

View full text Add to dashboard Cite

This study presents a theoretical analysis of the problems related to the inability of podocytes to proliferate. The basis of these problems is the very high rate of glomerular filtration. Podocytes do not in general die by apoptosis or necrosis but are lost by detachment from the glomerular basement membrane (GBM) as viable cells. Podocytes situated on the outside of the filtration barrier and attached to the GBM only by their foot processes are permanently exposed to the flow dynamic forces of the high filtration rate tending to detach them from the GBM. The major challenge seems to consist of the high shear stresses on the foot processes within the filtration slits due to filtrate flow. Healthy podocytes are able to resist this challenge, injured podocytes are not, and may undergo foot process detachment, leading to a gap in the podocyte cover of the GBM. This represents a mortal event. Like a dam break, such a leak cannot be repaired. The ongoing exposure to filtrate flow prevents any attempt to close the gap, thus preventing any regeneration including cell proliferation. An improvement of this precarious situation consists of healing by scarring that may involve only one lobule of the glomerulus, permitting the remaining lobules to maintain filtration. An answer to the question of which waste product requires such a high filtration rate for its excretion may be in the huge quantity of circulating peptides, a problem that dates far back in evolution.

show abstract

Morphological Processes of Foot Process Effacement in Puromycin Aminonucleoside Nephrosis Revealed by FIB/SEM Tomography

Cited by 31 publications

References 28 publications

Three-dimensional architecture of pericardial nephrocytes in Drosophila melanogaster revealed by FIB/SEM tomography

Three-dimensional architecture of pericardial nephrocytes in Drosophila melanogaster revealed by FIB/SEM tomography

Three-dimensional imaging of podocyte ultrastructure using FE-SEM and FIB-SEM tomography

The Inability of Podocytes to Proliferate: Cause, Consequences, and Origin

Contact Info

Product

Resources

About