Morphological studies of rat brain tumors induced by N-nitrosomethylurea

Schmidek, Henry H.; Nielsen, Surl L.; Schiller, Alan L.; Messer, J. R.

doi:10.3171/jns.1971.34.3.0335

Cited by 163 publications

(61 citation statements)

References 7 publications

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“…9L/Ro, a subline of the 9L N-methylnitrosourea-induced rat brain tumour (Schmidek et al, 1971), was grown as monolayer cell cultures in BME containing 10% FCS . Plating efficiencies of this cell line were determined on exponentially growing cell cultures 2 days after seeding 2 x 106 cells into 75Cm2 culture flasks.…”

Section: Materials and Aiethodsmentioning

confidence: 99%

Cell subpopulations dispersed from solid tumours and separated by centrifugal elutriation

Siemann¹,

Lord²,

Keng³

et al. 1981

Br J Cancer

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Summary.-The degree of non-neoplastic host-cell infiltration was assessed in 3 in vivo-in vitro tumour models commonly used in radiobiological studies: EMT6/Ro mammary carcinoma, 9L/Ro tumour and KHT sarcoma. While the 2 former tumour models have been shown to be moderately to highly immunogenic when grown s.c., the KHT sarcoma is apparently non-immunogenic. Using differential staining on single-cell suspensions from enzymatically dissociated solid tumours, all 3 tumour types were found to contain large proportions (3060%) of non-neoplastic host cells.The actual host-cell component found in the cell suspensions differed both in type and percentage for the 3 tumours studied. These host and neoplastic cells in the cell suspensions prepared from the solid tumours could readily be separated by centrifugal elutriation. After separation the clonogenic potential of the neoplastic cells was assessed, and was found to be higher than the clonogenic capacity of the unseparated cell suspension by a factor directly related to the host/neoplastic cell ratio. Even after the removal of the host cells, the clonogenic capacities of the neoplastic EMT6 and 9L tumour cells were lower than that of the corresponding in vitro sublines (,30 vs -75%). However, in the KHT sarcoma the removal of the host cell component raised the plating efficiency to .60°/,, which was similar to the value for the in vitro cell subline of this tumour.

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Section: Materials and Aiethodsmentioning

confidence: 99%

Cell subpopulations dispersed from solid tumours and separated by centrifugal elutriation

Siemann¹,

Lord²,

Keng³

et al. 1981

Br J Cancer

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“…Keywords 9L transplantable brain tumour; Fischer rats; template implantationj alternative The rat 9L gliosarcoma brain tumour model has been used extensively in studies of radiotherapy, chemotherapy and mechanisms of brain cancer for more than 20 years (Schmidek et al 1971, Swenberg et al 1972…”

Section: Discussionmentioning

confidence: 99%

“…Studies on rat cadavers showed no statistical difference in placement measurements between the stereotactic and template methods. These results indicate that the template method for intra cerebrally implanting tumour cells in rats provides a precise, accurate and rapid procedure that maximizes reproducibility with a significant reduction in labour requirements, when compared with the conventional stereotactic methodology.Keywords 9L transplantable brain tumour; Fischer rats; template implantationj alternative The rat 9L gliosarcoma brain tumour model has been used extensively in studies of radiotherapy, chemotherapy and mechanisms of brain cancer for more than 20 years (Schmidek et al 1971, Swenberg et al 1972 …”

mentioning

confidence: 99%

An alternative method to stereotactic inoculation of transplantable brain tumours in large numbers of rats

et al. 2000

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SummaryThe rat 9L gliosarcoma brain tumour model has been widely used in brain cancer studies. Intracerebral implantation of the cells in the parietal lobe of the brain has been performed using the stereotactic or freehand inoculation methods. For large numbers of rats, we wished to develop a method more accurate and precise than the freehand method, but less labour intensive than the stereotactic method. A template implantation technique was developed and compared quantitatively with the stereotactic method. Rats were inoculated with either the template or stereotactic method at doses of 1000, 5000, 10 000, 20 000 or 40 000 cells. Results of this comparison showed that the template method is precise and accurate for tumour placement within the brain cortex, and decreases labour requirements. Mean survival rates between groups were not significantly different at doses of 5000, 20 000 or 40 000 cells inoculated. Significance was seen at the low dose of 1000 cells (P < 0.001). This was attributable to an absence of tumour growth in five of six stereotactic rats in this group. Significance was also seen at the 10 000 dose level (P < 0.05) with the stereotactic rats again surviving longer than the template rats. However, in this case all the stereotactic rats had tumour growth. Brain weights did not differ significantly between groups, except at the 1000 dose level where no growth of tumour occurred in five of the six stereotactic animals. Body weight gain within one week following surgery did not differ significantly between any of the groups at rJ. = 0.05. Studies on rat cadavers showed no statistical difference in placement measurements between the stereotactic and template methods. These results indicate that the template method for intra cerebrally implanting tumour cells in rats provides a precise, accurate and rapid procedure that maximizes reproducibility with a significant reduction in labour requirements, when compared with the conventional stereotactic methodology.Keywords 9L transplantable brain tumour; Fischer rats; template implantationj alternative The rat 9L gliosarcoma brain tumour model has been used extensively in studies of radiotherapy, chemotherapy and mechanisms of brain cancer for more than 20 years (Schmidek et al. 1971, Swenberg et al. 1972

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“…It carries a very poor prognosis with mean overall survival of [12][13][14][15][16][17] months. Despite testing a multitude of chemotherapy agents and strategies, very little progress has been made in extending the life expectancy of those diagnosed with a GBM, since the establishment of the Stupp protocol (a combination of radiotherapy and temozolomide) a decade ago [2,3].…”

Section: Introductionmentioning

confidence: 99%

“…The C6 glioma cell line was grown out from a glioma induced in an outbred Wistar rat strain by repeated rou ds of treat e t ith N'N'-methylnitrosourea [11,12]. Injection of the C6 cell line into newborn rats produced a tumour with pathology consistent with a glioblastoma multiforme including a diffuse invading border [13].…”

Section: Introductionmentioning

confidence: 99%