Morphological study of equine amniotic compartment

Lanci, Aliai; Ingrà, Laura; Dondi, Francesco; Tomasello, Federico; Teti, Gabriella; Mariella, Jole; Falconi, Mirella; Castagnetti, Carolina

doi:10.1016/j.theriogenology.2021.10.019

Cited by 7 publications

(9 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…If parturition was eutocic it was assisted without intervention. The time of the rupture of chorioallantois was recorded; after the appearance of the amniotic membrane through the vulva, amniotic fluid was collected via needle puncture using a 60-mL sterile syringe [ 20 , 21 ], and amniotic lactate concentration (mmol/L) (Lactate SCOUT+, SensLab, GmbH, Lepzig, Germany) [ 20 ] was measured.…”

Section: Methodsmentioning

confidence: 99%

Dystocia in the Standardbred Mare: A Retrospective Study from 2004 to 2020

Lanci

Perina²,

Donadoni³

et al. 2022

Animals

Self Cite

View full text Add to dashboard Cite

Dystocia as a prolonged stage II parturition (>30 min) was associated with a higher risk of complications. The hypothesis of the study was that any type of dystocia could affect the foal’s health, even when the stage II was <30 min. Clinical reports on 222 Standardbred mares and their foals hospitalized at the Veterinary Teaching Hospital of the University of Bologna from 2004 to 2020 were reviewed. Mares were divided into the Eutocia Group (165, eutocic delivery) and the Dystocia Group (57, dystocic delivery). The incidence of dystocia was 4.9%. Stage II was longer in the Dystocia Group (median 20 min) than in the Eutocia Group (median 12 min). All occurrences of dystocia were retrospectively classified into three categories of severity: mild, moderate and severe dystocia. The occurrence of postpartum complications in mares and neonatal diseases and failure of passive transfer of immunity in foals was higher in the Dystocia Group. Foal venous lactatemia and serum creatine kinase were significantly higher in the Dystocia Group (median 3.9 mmol/L; 262 UI/L respectively) than in the Eutocia Group (median 3.1 mmol/L; 187 UI/L respectively). The APGAR score was lower in the Dystocia Group (median 8) than in the Eutocia Group (median 10) and significantly lower in severe dystocia (median 3). The duration of stage II should not be considered the only parameter of dystocia in mares: even a rapid resolution of dystocia could pose health risks to the foal and the mare.

show abstract

Section: Methodsmentioning

confidence: 99%

Dystocia in the Standardbred Mare: A Retrospective Study from 2004 to 2020

Lanci

Perina²,

Donadoni³

et al. 2022

Animals

Self Cite

View full text Add to dashboard Cite

show abstract

“…At term, the extensive allantochorion consists of no more than a single thin layer of low columnar-to-cuboidal trophoblast cells which overlies and provides the essential structural framework for an incredibly densely packed mass of fetal capillaries supported in minimal amounts of allantoic mesoderm [13]. The amnion is the inner fetal membrane and consists of a cuboid epithelial layer that comes into direct contact with the amniotic fluid [45].…”

Section: Discussionmentioning

confidence: 99%

Study on NGF and VEGF during the Equine Perinatal Period—Part 1: Healthy Foals Born from Normal Pregnancy and Parturition

Ellero

Lanci

Baldassarro

et al. 2022

Veterinary Sciences

Self Cite

View full text Add to dashboard Cite

The importance of trophic factors, such as nerve growth factor (NGF), vascular endothelial growth factor (VEGF), and brain-derived neurotrophic factor (BDNF) during the perinatal period, is now emerging. Through their functional activities of neurogenesis and angiogenesis, they play a key role in the final maturation of the nervous and vascular systems. The present study aims to: (i) evaluate the NGF and VEGF levels obtained at parturition from the mare, foal and umbilical cord vein plasma, as well as in amniotic fluid; (ii) evaluate NGF and VEGF content in the plasma of healthy foals during the first 72 h of life (T0, T24 and T72); (iii) evaluate NGF and VEGF levels at parturition in relation to the selected mares’ and foals’ clinical parameters; (iv) evaluate the relationship between the two trophic factors and the thyroid hormone levels (TT3 and TT4) in the first 72 h of life; (v) assess mRNA expression of NGF, VEGF and BDNF and their cell surface receptors in the placenta. Fourteen Standardbred healthy foals born from mares with normal pregnancies and parturitions were included in the study. The dosage of NGF and VEGF levels was performed using commercial ELISA kits, whereas NGF, VEGF and BDNF placental gene expression was performed using semi-quantitative real-time PCR. In foal plasma, both NGF and VEGF levels decreased significantly over time, from T0 to T24 (p = 0.0066 for NGF; p < 0.0001 for VEGF) and from T0 to T72 (p = 0.0179 for NGF; p = 0.0016 for VEGF). In foal serum, TT3 levels increased significantly over time from T0 to T24 (p = 0.0058) and from T0 to T72 (p = 0.0013), whereas TT4 levels decreased significantly over time from T0 to T24 (p = 0.0201) and from T0 to T72 (p < 0.0001). A positive correlation was found in the levels of NGF and VEGF in foal plasma at each time point (p = 0.0115; r = 0.2862). A positive correlation was found between NGF levels in the foal plasma at T0 and lactate (p = 0.0359; r = 0.5634) as well as between VEGF levels in the foal plasma at T0 and creatine kinase (p = 0.0459; r = 0.5407). VEGF was expressed in all fetal membranes, whereas NGF and its receptors were not expressed in the amnion. The close relationship between the two trophic factors in foal plasma over time and their fine expression in placental tissues appear to be key regulators of fetal development and adaptation to extra-uterine life.

show abstract

“…Amnion consists of an epithelium made up of a monolayer of cuboidal epithelial cells, a basement membrane, and a mesenchymal matrix. 6,11,19,20 This structure is common to both human and equine amnion. 19 The epithelium is the innermost of these 3 layers and is in contact with amniotic fluid during gestation.…”

mentioning

confidence: 99%

“…19 The epithelium is the innermost of these 3 layers and is in contact with amniotic fluid during gestation. 6,20 The epithelial cells in human and equine amnion are covered in microvilli on their apical surface. 6,20,21 These cells are presumed to have an active secretory function 6 and are recognized as a source of many cytokines and growth factors.…”

mentioning

confidence: 99%

“…6,20 The epithelial cells in human and equine amnion are covered in microvilli on their apical surface. 6,20,21 These cells are presumed to have an active secretory function 6 and are recognized as a source of many cytokines and growth factors. 6,7,22 Concentrations of various cytokines and growth factors in human amnion were significantly lower when the epithelium was removed prior to analysis, supporting the secretory role of epithelial cells.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Healing time of experimentally induced distal limb wounds in horses is not reduced by local injection of equine-origin liquid amnion allograft

Duddy

Schoonover

Williams

et al. 2022

ajvr

View full text Add to dashboard Cite

OBJECTIVE To assess the efficacy of an equine-origin liquid amnion allograft (ELAA) derived from both amniotic fluid and amniotic membrane on the healing time of experimentally induced distal limb wounds in horses. ANIMALS 8 adult horses. PROCEDURES On day 0, horses were anesthetized and a 2.5 X 2.5-cm, full-thickness skin wound was created on the dorsal aspect of each metacarpus and bandaged. On day 9, wound margins were injected with ELAA (treatment) or 0.9% NaCl (control). Bandages were changed at specific intervals through day 91 and, on each occasion, wounds were photographed to allow calculation of wound area. Exuberant granulation tissue was resected, if present. Wounds were deemed healed when completely epithelialized. Mean wound area was compared between groups throughout the study period. RESULTS Only 1 wound (control) remained unhealed at day 91. No difference was found between the treatment and control groups in either wound area over time (P = 1.0) or time for wounds to reduce in size by 95% (P = .2) Exuberant granulation tissue required resection twice (1 control wound and 1 treatment wound). CLINICAL RELEVANCE In this model, a single treatment with ELAA administered locally by SC injection did not accelerate distal limb wound healing in horses. However, it is possible that naturally occurring, chronic, or nonhealing wounds would respond differently.

show abstract

Morphological study of equine amniotic compartment

Cited by 7 publications

References 42 publications

Dystocia in the Standardbred Mare: A Retrospective Study from 2004 to 2020

Dystocia in the Standardbred Mare: A Retrospective Study from 2004 to 2020

Study on NGF and VEGF during the Equine Perinatal Period—Part 1: Healthy Foals Born from Normal Pregnancy and Parturition

Healing time of experimentally induced distal limb wounds in horses is not reduced by local injection of equine-origin liquid amnion allograft

Contact Info

Product

Resources

About