Morphological study of thymus stromal cells (TEL-2 cell) which play a role in the elimination of double positive immature thymocytes by phagocytosis

Kawabuchi, Masaru; Nakamura, Kei‐ichiro; Hirata, Ken‐ichi; Mori, Keiko; Nakashima, Mitsuyoshi; Kishi, Hiroyuki; Islam, Sameer; Zhou, Chong-Jian; Watanabe, Takeshi

doi:10.1002/(sici)1097-0185(199603)244:3<271::aid-ar1>3.0.co;2-1

Cited by 3 publications

References 36 publications

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Synthesis of Functional Tertiary Lymphoid Organs

Kobayashi

Kato

Nakamura

et al. 2016

Synthetic Immunology

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The regeneration of functional immune organ will be one of major focus in future immunology research. It will be an useful tool which induces efficient immune responses in the body on demand and offers effective ways to restore the immune status and treat uncontrollable obstinate diseases such as cancer, autoimmune diseases, severe infection and immuno-insufficiency/deficiency caused by tissue damages, abnormality, primary defect and aging. Artificially synthesized lymphoid organs may also provide us with a highly informative method not only for clinical aim but also basic study on the development and functions of immunological tissues and organs. We first reported successful generation of artificiallyconstructed lymph node-like tertiary lymphoid tissues at ectopic sites in mouse by applying certain stromal cell lines (Suematsu S, Watanabe T. Nat Biotechnol 22 (11):422-428, 2010). They showed a remarkable ability to induce immune responses upon antigen stimulation, especially when transplanted into naïve or immune-compromised hosts. In this review, we discuss about the rationale and method for the synthesis of functional tertiary lymph node-like lymphoid tissues in mouse. Especially, we discuss here on the method with applying only soluble factors but without using any stromal cell, that enables proper accumulation and functional organization of immune cells in grafts.

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Synthesis of Functional Tertiary Lymphoid Organs

Kobayashi

Kato

Nakamura

et al. 2016

Synthetic Immunology

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Detection of DNA fragmentation in human breast cancer tissue by an antibody specific to single-stranded DNA

et al. 1998

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While there have been many reports concerning the clinical significance of bcl-2 expression in human breast cancer, little is known about apoptosis in primary breast cancers. We immunohistochemically examined DNA fragmentation in 107 primary human breast cancers from Japanese women using an antibody specific to single-stranded DNA. The apoptosis index calculated as the product of the positive cell number and the cellularity coefficient, ranged from 0 to 48. The average incidence of apoptosis was calculated as 0.1% of tumor cells. No relationships were observed among the apoptosis index, expression of bcl-2, and the histological grade of the tumors. Almost all apoptotic cells were phagocytosed by surrounding tumor cells immediately after DNA fragmentation. Apoptotic body formation was rare. The apoptotic cells seemed to be degraded within phagocytes, leaving no trace of apoptosis except the tiny shells of nuclei. The intensive phagocytic reaction might be one of the main reasons for the low incidence of apoptosis in human breast cancers.

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Different Roles of a Rat Cortical Thymic Epithelial Cell Line In Vitro on Thymocytes and Thymocyte Hybridoma Cells: Phagocytosis, Induction of Apoptosis, Nursing and Growth Promoting Activities

Vučević¹,

Čolić²,

Popović³

et al. 2002

Journal of Immunology Research

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In this work, the interaction between a rat cortical thymic epithelial cell (TEC) line (R-TNC.1) with nursing activity and thymocytes as well as BWRT 8 thymocyte hybridoma (TH) cells has been studied. The R-TNC.1 cell line significantly bound thymocytes and TH. Binding was stronger during the first 30 min of cell incubation and was followed by a progressive deadhesion. Among adherent thymocytes the proportion of apoptotic cells increased with culture time which was a consequence of higher capacity of the line for binding of apoptotic than viable cells and induction of apoptosis in a subset of adherent thymocytes. Emperiopolesis activity of this thymic nurse cell (TNC) line was manifested by engulfment of thymocytes as well as TH cells. A subset of viable intra-TNC thymocytes has been triggered to die by apoptosis, whereas other internalized thymocytes have been stimulated to proliferate, as measured by an increase in the percentage of cells in mitosis and higher incorporation of bromodeoxyuridine (BrdU), in comparison to thymocytes cultivated alone. A significant stimulation of proliferation of engulfed TH cells was also observed. The R-TNC.1 cell line efficiently phagocytosed both apoptotic thymocytes and TH, and the process is followed by intra-TNC destruction of ingested cells. Cumulatively, these results suggest different role of the R-TNC.1 clone: phagocytosis of apoptotic cells; induction of apoptotic cell death in a subset of both bound and internalized thymocytes and stimulation of proliferation of a subset of intra-TNC thymocytes or TH cells.

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Morphological study of thymus stromal cells (TEL-2 cell) which play a role in the elimination of double positive immature thymocytes by phagocytosis

Cited by 3 publications

References 36 publications

Synthesis of Functional Tertiary Lymphoid Organs

Synthesis of Functional Tertiary Lymphoid Organs

Detection of DNA fragmentation in human breast cancer tissue by an antibody specific to single-stranded DNA

Different Roles of a Rat Cortical Thymic Epithelial Cell Line In Vitro on Thymocytes and Thymocyte Hybridoma Cells: Phagocytosis, Induction of Apoptosis, Nursing and Growth Promoting Activities

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