Morphological Type Identification of Self-Incompatibility in Dendrobium and Its Phylogenetic Evolution Pattern

Niu, Shan-Ce; Huang, Jie; Chen, Nansheng; Li, Pei-Xing; Yang, Haijun; Zhang, Yongqiang; Zhang, Guoqiang; Chen, Lijun; Niu, Yunxia; Luo, Yan; Liu, Zhong‐Jian

doi:10.3390/ijms19092595

Cited by 18 publications

(13 citation statements)

References 29 publications

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“…Among those barcoding regions, ITS was the most commonly used. [2,8,9,14,15,25,[36][37][38][39]. Our results again confirmed the effect of ITS in the evaluation of genetic diversity and the identification of Dendrobium species not only in Southern Vietnam but also in other habitats.…”

Section: Discussionsupporting

confidence: 81%

Molecular Identification and Evaluation of the Genetic Diversity of Dendrobium Species Collected in Southern Vietnam

Nguyen

et al. 2020

Biology

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Dendrobium has been widely used not only as ornamental plants but also as food and medicines. The identification and evaluation of the genetic diversity of Dendrobium species support the conservation of genetic resources of endemic Dendrobium species. Uniquely identifying Dendrobium species used as medicines helps avoid misuse of medicinal herbs. However, it is challenging to identify Dendrobium species morphologically during their immature stage. Based on the DNA barcoding method, it is now possible to efficiently identify species in a shorter time. In this study, the genetic diversity of 76 Dendrobium samples from Southern Vietnam was investigated based on the ITS (Internal transcribed spacer), ITS2, matK (Maturase_K), rbcL (ribulose-bisphosphate carboxylase large subunit) and trnH-psbA (the internal space of the gene coding histidine transfer RNA (trnH) and gene coding protein D1, a polypeptide of the photosystem I reaction center (psaB)) regions. The ITS region was found to have the best identification potential. Nineteen out of 24 Dendrobium species were identified based on phylogenetic tree and Indel information of this region. Among these, seven identified species were used as medicinal herbs. The results of this research contributed to the conservation, propagation, and hybridization of indigenous Dendrobium species in Southern Vietnam.

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Section: Discussionsupporting

confidence: 81%

Molecular Identification and Evaluation of the Genetic Diversity of Dendrobium Species Collected in Southern Vietnam

Nguyen

et al. 2020

Biology

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“…The typical GSI reactions that can lead to PCD in pollen tubes are based on S-RNase proteins in the pistil and F-box proteins in the microgametophyte in Solanaceae [ 5 , 38 , 39 ] as well as a Ca 2+ -dependent signaling cascade in Papaveraceae [ 40 ]. In the case of self-incompatible Orchidaceae species, molecular studies have not revealed the presence of S-RNases, suggesting that a distinct molecular control of GSI could occur in the family [ 41 , 42 ]. Additionally, there are no records involving a Ca 2+ -dependent signaling cascade in the death of pollen tubes in Orchidaceae.…”

Section: Discussionmentioning

confidence: 99%

Two Self-Incompatibility Sites Occur Simultaneously in the Same Acianthera Species (Orchidaceae, Pleurothallidinae)

Duarte

Oliveira

Borba

2020

Plants

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In most species of Pleurothallidinae, the self-incompatibility site occurs in the stylar canal inside the column, which is typical of gametophytic self-incompatibility (GSI). However, in some species of Acianthera, incompatible pollen tubes with anomalous morphology reach the ovary, as those are obstructed in the column. We investigated if a distinct self-incompatibility (SI) system is acting on the ovary of A. johannensis, which is a species with partial self-incompatibility, contrasting with a full SI species, A. fabiobarrosii. We analyzed the morphology and development of pollen tubes in the column, ovary, and fruit using light, epifluorescence, and transmission electron microscopy. Our results show that the main reaction site in A. johannensis is in the stylar canal inside the column, which was also recorded in A. fabiobarrosii. Morphological and cytological characteristics of the pollen tubes with obstructed growth in the column indicated a process of programmed cell death in these tubes, showing a possible GSI reaction. In addition, partially self-incompatible individuals of A. johannensis exhibit a second SI site in the ovary. We suggest that this self-incompatibility site in the ovary is only an extension of GSI that acts in the column, differing from the typical late-acting self-incompatibility system recorded in other plant groups.

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“…SI-related patents have also been issued in species with unknown SI genetics. For instance, Dendrobium species are of interest as ornamental and medicinal plants, but show a high rate of SI and the system has not been characterized (Niu et al, 2018). Thus, in Dendrobium officinale (Orchidaceae) a method has been patented for overcoming SI using a pollination accelerator based on indole acetic acid (Xin et al, 2017).…”

Section: Patented Applications Of Plant Si Systemsmentioning

confidence: 99%

Self-(In)compatibility Systems: Target Traits for Crop-Production, Plant Breeding, and Biotechnology

et al. 2020

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Self-incompatibility (SI) mechanisms prevent self-fertilization in flowering plants based on specific discrimination between self-and non-self pollen. Since this trait promotes outcrossing and avoids inbreeding it is a widespread mechanism of controlling sexual plant reproduction. Growers and breeders have effectively exploited SI as a tool for manipulating domesticated crops for thousands of years. However, only within the past thirty years have studies begun to elucidate the underlying molecular features of SI. The specific S-determinants and some modifier factors controlling SI have been identified in the sporophytic system exhibited by Brassica species and in the two very distinct gametophytic systems present in Papaveraceae on one side and in Solanaceae, Rosaceae, and Plantaginaceae on the other. Molecular level studies have enabled SI to SC transitions (and vice versa) to be intentionally manipulated using marker assisted breeding and targeted approaches based on transgene integration, silencing, and more recently CRISPR knock-out of SI-related factors. These scientific advances have, in turn, provided a solid basis to implement new crop production and plant breeding practices. Applications of self-(in)compatibility include widely differing objectives such as crop yield and quality improvement, marker-assisted breeding through SI genotyping, and development of hybrids for overcoming intra-and interspecific reproductive barriers. Here, we review scientific progress as well as patented applications of SI, and also highlight future prospects including further elucidation of SI systems, deepening our understanding of SI-environment relationships, and new perspectives on plant self/non-self recognition.

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Morphological Type Identification of Self-Incompatibility in Dendrobium and Its Phylogenetic Evolution Pattern

Cited by 18 publications

References 29 publications

Molecular Identification and Evaluation of the Genetic Diversity of Dendrobium Species Collected in Southern Vietnam

Molecular Identification and Evaluation of the Genetic Diversity of Dendrobium Species Collected in Southern Vietnam

Two Self-Incompatibility Sites Occur Simultaneously in the Same Acianthera Species (Orchidaceae, Pleurothallidinae)

Self-(In)compatibility Systems: Target Traits for Crop-Production, Plant Breeding, and Biotechnology

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