Morphology and contractile gene expression of adipose-derived mesenchymal stem cells in response to short-term cyclic uniaxial strain and TGF-β1

Rashidi, Neda; Tafazzoli-Shadpour, Mohammad; Haghighipour, Nooshin; Khani, Mohammad‐Mehdi

doi:10.1515/bmt-2016-0228

Cited by 9 publications

(3 citation statements)

References 36 publications

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“…Furthermore, cells respond to different modes, frequencies, and magnitudes of mechanobiological stimuli with distinct behavior. For example, MSCs exposed to shear stress express endothelial phenotypes, whereas MSCs exposed to uniaxial stretch, but not equiaxial stretch, express vascular SMC phenotypes [50,53,54]. Stretch frequencies up to 1 Hz induce a contractile phenotype in MSCs and SMCs, whereas static conditions and higher frequencies do not [38,39,55,56].…”

Section: Multi-axial Mechanical Preconditioning For Future Tissue Bio...mentioning

confidence: 99%

Soft pneumatic actuators for mimicking multi-axial femoropopliteal artery mechanobiology

Fell¹,

Brooks-Richards²,

Woodruff³

et al. 2022

Biofabrication

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Tissue biomanufacturing aims to produce lab-grown stem cell grafts and biomimetic drug testing platforms but remains limited in its ability to recapitulate native tissue mechanics. The emerging field of soft robotics aims to emulate dynamic physiological locomotion, representing an ideal approach to recapitulate physiologically complex mechanical stimuli and enhance patient-specific tissue maturation. The kneecap’s femoropopliteal artery (FPA) represents a highly flexible tissue across multiple axes during blood flow, walking, standing, and crouching positions, and these complex biomechanics are implicated in the FPA’s frequent presentation of peripheral artery disease. We developed a soft pneumatically actuated (SPA) cell culture platform to investigate how patient-specific FPA mechanics affect lab-grown arterial tissues. Silicone hyperelastomers were screened for flexibility and biocompatibility, then additively manufactured into SPAs using a simulation-based design workflow to mimic normal and diseased FPA extensions in radial, angular, and longitudinal dimensions. SPA culture platforms were seeded with mesenchymal stem cells, connected to a pneumatic controller, and provided with 24-hour multi-axial exercise schedules to demonstrate the effect of dynamic conditioning on cell alignment, collagen production, and muscle differentiation without additional growth factors. Soft robotic bioreactors are promising platforms for recapitulating patient-, disease-, and lifestyle-specific mechanobiology for understanding disease, treatment simulations, and lab-grown tissue grafts.

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Section: Multi-axial Mechanical Preconditioning For Future Tissue Bio...mentioning

confidence: 99%

Soft pneumatic actuators for mimicking multi-axial femoropopliteal artery mechanobiology

Fell¹,

Brooks-Richards²,

Woodruff³

et al. 2022

Biofabrication

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“…However, the exact mechanisms by which cells sense and respond to local mechanical signals are not well understood [24–27]. Mechanotransduction occurs in living cells by various stimuli such as hydrostatic pressure [28–31], cyclic stretch [32–35], and cyclic shear stress [36–38].…”

Section: Introductionmentioning

confidence: 99%

Enhanced gene delivery in tumor cells using chemical carriers and mechanical loadings

et al. 2018

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Intracellular delivery of DNA is considered a challenge in biological research and treatment of diseases. The previously reported transfection rate by commercially available transfection reagents in cancer cell lines, such as the mouse lung tumor cell line (TC-1), is very low. The purpose of this study is to introduce and optimize an efficient gene transfection method by mechanical approaches. The combinatory transfection effect of mechanical treatments and conventional chemical carriers is also investigated on a formerly reported hard-to-transfect cell line (TC-1). To study the effect of mechanical loadings on transfection rate, TC-1 tumor cells are subjected to uniaxial cyclic stretch, equiaxial cyclic stretch, and shear stress. The TurboFect transfection reagent is exerted for chemical transfection purposes. The pEGFP-N1 vector encoding the green fluorescent protein (GFP) expression is utilized to determine gene delivery into the cells. The results show a significant DNA delivery rate (by ~30%) in mechanically transfected cells compared to the samples that were transfected with chemical carriers. Moreover, the simultaneous treatment of TC-1 tumor cells with chemical carriers and mechanical loadings significantly increases the gene transfection rate up to ~ 63% after 24 h post-transfection. Our results suggest that the simultaneous use of mechanical loading and chemical reagent can be a promising approach in delivering cargoes into cells with low transfection potentials and lead to efficient cancer treatments.

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“…The main tools for differentiating ASCs toward VSMCs include composing cell culture media and exerting mechanical stress in dynamic cell culture systems, similarly as for the re-differentiation of VSMCs. Examples of results obtained by various authors[10,11,[58][59][60][61][62][63][64][65][66][67] are summarized inTable 1. Culture conditions for differentiation of ASCs into VSMCs and the obtained markers of differentiation.…”

mentioning

confidence: 99%

Vascular Smooth Muscle Cells (VSMCs) in Blood Vessel Tissue Engineering: The Use of Differentiated Cells or Stem Cells as VSMC Precursors

Bačáková¹,

Trávníčková²,

Filová³

et al. 2018

Muscle Cell and Tissue - Current Status of Research Field

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Vascular smooth muscle cells (VSMCs) play important roles in the physiology and pathophysiology of the blood vessels. In a healthy adult organism, VSMCs are quiescent, but after a blood vessel injury, they undergo phenotypic modulation from the contractile phenotype to the synthetic phenotype, characterized by high activity in migration, proliferation and proteosynthesis. This behavior of VSMCs can lead to stenosis or obliteration of the vascular lumen. For this reason, VSMCs have tended to be avoided in the construction of blood vessel replacements. However, VSMCs are a physiological and the most numerous component of blood vessels, so their presence in novel advanced vascular replacements is indispensable. Either differentiated VSMCs or stem cells as precursors of VSMCs can be used in the reconstruction of the tunica media in these replacements. VSMCs can be obtained from blood vessels (usually from subcutaneous veins) taken surgically from the patients and can be expanded in vitro. During in vitro cultivation, VSMCs lose their differentiation markers, at least partly. These cells should therefore be re-differentiated by seeding them on appropriate scaffolds by composing cell culture media and by mechanical stimulation in dynamic bioreactors. Similar approaches can also be applied for differentiating stem cells, particularly adipose tissue-derived stem cells, toward VSMCs for the purposes of vascular tissue engineering.

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Morphology and contractile gene expression of adipose-derived mesenchymal stem cells in response to short-term cyclic uniaxial strain and TGF-β1

Cited by 9 publications

References 36 publications

Soft pneumatic actuators for mimicking multi-axial femoropopliteal artery mechanobiology

Soft pneumatic actuators for mimicking multi-axial femoropopliteal artery mechanobiology

Enhanced gene delivery in tumor cells using chemical carriers and mechanical loadings

Vascular Smooth Muscle Cells (VSMCs) in Blood Vessel Tissue Engineering: The Use of Differentiated Cells or Stem Cells as VSMC Precursors

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