Morphology and molecular study of three new Cordycipitoid fungi and its related species collected from Jilin Province, northeast China

Abstract

Cordyceps species are notable medicinal fungi in China, which are pathogenic on insects and exhibit high biodiversity in tropical and subtropical regions. Recently, three new Cordyceps species, Cordyceps changchunensis and Cordyceps jingyuetanensis growing on pupae of Lepidoptera and Cordyceps changbaiensis growing on larvae of Lepidoptera, were found in Jilin Province, China and are described, based on morphological and ecological characteristics. These three new species are similar to the Cordyceps militaris group, but are distinctly distinguishable from the known species. Cordyceps changchunensis, characterised by its small and light yellow to orange stromata which is occasionally forked, covered with white mycelium at the base of stipe, globose to ovoid perithecia, is macroscopically similar to Cordyceps militaris. Cordyceps changbaiensis is clearly discriminated from other Cordyceps species by its white to orange and branched stromata, clavate to cylindrical fertile apical portion, immersed and globose to ovoid perithecia. Moreover, unbranched, clavate and orange to light red stromata, almond-shaped to ovoid and immersed perithecia separate Cordyceps jingyuetanensis from other Cordyceps species. nrITS, nrLSU and EF-1α sequences were undertaken and phylogenetic trees, based on Maximum Likelihood and Bayesian Inference analysis showed that the three new species clustered with Cordyceps militaris, but formed individual clades, as well as confirmed the results of our morphological study.

Ecological Factors Influencing the Occurrence of Macrofungi from Eastern Mountainous Areas to the Central Plains of Jilin Province, China

“…Xie, and T.Z. Wei [ 20 ]; Cordyceps changchunensis J.J. Hu, Bo Zhang, and Y. Li; Cordyceps changbaiensis J.J. Hu, Bo Zhang, and Y. Li; and Cordyceps jingyuetanensis J.J. Hu, Bo Zhang, and Y. Li [ 21 ]. However, the macrofungi distribution patterns and the relationship with the environmental factors are currently unclear.…”

Morphology and Phylogeny Reveal Five Novel Species in the Genus Cordyceps (Cordycipitaceae, Hypocreales) From Yunnan, China

“…To date, together with the species as reported in recent years, there are about 70 species whose phylogenetic positions are known in Cordyceps worldwide ( Tasanathai et al, 2016 ; Chirivi et al, 2017 ; Catania et al, 2018 ; Mongkolsamrit et al, 2018 , 2020 ; Olatunji et al, 2018 ; Crous et al, 2019 ; Hyde et al, 2019 ; Li et al, 2020 ; Wang Y. B. et al, 2020 ; Hu et al, 2021 ).…”

Multiple evidences reveal new species and a new record of smelly Gymnopus (Agaricales, Omphalotaceae) from China

“…PCR reactions (25 μL) contained 8 μL 2 × EasyTaq ® PCR SuperMix (TransGen Biotech Co., Ltd., Beijing, China), 1 μL 10 μM primer L, 1 μL 10 μM primer R, 3 μL DNA solution, and 12 μL dd H 2 O (Hu et al, 2021a , 2022 ). The reaction programs were as follows: for ITS, initial denaturation at 94°C for 4 min, followed by 30 cycles at 94°C for 1 min, 54°C for 1 min and 72°C for 1 min, and a final extension of 72°C for 10 min (Coimbra et al, 2015 ); for nLSU, initial denaturation at 95°C for 3 min, followed by 30 cycles at 94°C for 30 s, 47°C for 45 s, and 72°C for 90 s, and a final extension of 72°C for 10 min (Ryoo et al, 2016 ; Hu et al, 2021b ). The PCR products were visualized via UV light after electrophoresis on 1% agarose gels stained with ethidium bromide and purified using the Genview High-Efficiency Agarose Gels DNA Purification Kit (Gen-View Scientific Inc., Galveston, TX, USA).…”

“…Impudicae (Kirk et al, 2008 ). In China, the species diversity, taxonomy, and phylogeny of macrofungi have been investigated in recent years, thus many new species have been discovered (Zhong et al, 2018 ; Cui et al, 2019 ; Sun et al, 2020 , 2022 ; Zhang et al, 2020 ; Cao et al, 2021a , b ; Hu et al, 2021b ; Liu et al, 2021 , 2022a , b ; Ji et al, 2022 ; Tuo et al, 2022 ; Wang et al, 2022 ). However, research on sect.…”