Morphology and ultrastructure of the germarium in panoistic ovarioles of a basal “apterygotous” insect, Thermobia domestica

Tworzydło, Wacław; Kisiel, Elzbieta; Jankowska, Władysława; Biliński, S

doi:10.1016/j.zool.2014.01.002

Cited by 29 publications

(32 citation statements)

References 38 publications

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“…The germaria of Thermobia are relatively short and contain germline cells in various developmental stages, i.e., cystoblasts, early meiotic (leptotene–diploten) oocytes, and previtellogenic (diktiotene) oocytes, as well as small somatic cells (Tworzydlo et al 2014). The chromatin of somatic cells is highly condensed and stains with the methylene blue (Epon sections), Giemsa, and Hoechst (squash preparations) (Figs.…”

Section: Resultsmentioning

confidence: 99%

“…Simultaneously, to the gradual formation of the chromosome bouquet in the nucleoplasm, rearrangement of organelles within the ooplasm (oocyte cytoplasm) takes place. This process leads to the formation of the complex assemblage of organelles known as the Balbiani body (Tworzydlo et al 2014). During the next stage, i.e., the diplotene, the bouquet disappears, the chromosomes become progressively shorter and markedly thicker (0.6–0.7 μm) (Figs.…”

Section: Resultsmentioning

confidence: 99%

“…The female reproductive system of Thermobia domestica is composed of two ovaries attached to paired lateral oviducts that join together to form a single common oviduct (Tworzydlo et al 2014). Each of the ovaries is composed of five ovarioles of the panoistic type.…”

Section: Introductionmentioning

confidence: 99%

“…Our previous studies have shown that in Thermobia the germaria contain three categories of germline cells, namely the cystoblasts (progenitor cells of the oocytes), early meiotic and previtellogenic oocytes, and that the chromosomes of post leptotene oocytes gather in one region of the nucleoplasm where they form the so-called bouquet (Tworzydlo et al 2014; see Harper et al 2004; Tomita and Cooper 2006; Chikashige et al 2006; Ding et al 2007 for further reading). We have shown additionally that in concert to the formation of the chromosome bouquet, rearrangement of organelles within the ooplasm (oocyte cytoplasm) takes place, leading to the formation of a complex organelle assemblage, known as the Balbiani body (Tworzydlo et al 2014; for a review of the Balbiani body morphology and functioning see Kloc et al 2004a, 2014; Pepling et al 2007; Marlow and Mullins 2008). Interestingly, the Balbiani body locates invariably next to this segment of the nuclear envelope to which the telomeres of the bouquet chromosomes are attached (Tworzydlo et al 2014).…”

Section: Introductionmentioning

confidence: 99%

“…We have shown additionally that in concert to the formation of the chromosome bouquet, rearrangement of organelles within the ooplasm (oocyte cytoplasm) takes place, leading to the formation of a complex organelle assemblage, known as the Balbiani body (Tworzydlo et al 2014; for a review of the Balbiani body morphology and functioning see Kloc et al 2004a, 2014; Pepling et al 2007; Marlow and Mullins 2008). Interestingly, the Balbiani body locates invariably next to this segment of the nuclear envelope to which the telomeres of the bouquet chromosomes are attached (Tworzydlo et al 2014). In this context, we decided to analyze sequential changes within the nucleoplasm during early stages of meiotic division in this insect.…”

Section: Introductionmentioning

confidence: 99%

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Meiosis, Balbiani body and early asymmetry of Thermobia oocyte

et al. 2016

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The meiotic division guarantees maintenance of a genetic diversity; it consists of several stages, with prophase I being the longest and the most complex. We decided to follow the course of initial stages of meiotic division in ovaries of Thermobia domestica using modified techniques of squash preparations, semithin sections, and electron microscopy. We show that germaria contain numerous germline cells that can be classified into three categories: cystoblasts, meiotic oocytes, and growing previtellogenic oocytes. The cystoblasts are located most apically. The meiotic oocytes occupy the middle part of the germarium, and the previtellogenic oocytes can be found in the most basal part, near the vitellarium. Analyses of the semithin sections and squash preparations show that post leptotene meiotic chromosomes gather in one region of the nucleoplasm where they form the so-called bouquet. The telomeres of the bouquet chromosomes are attached to a relatively small area (segment) of the nuclear envelope. Next to this envelope segment, the nucleolar organizers are also located. We show that in concert to sequential changes inside the oocyte nuclei, rearrangement of organelles within the ooplasm (oocyte cytoplasm) takes place. This leads to the formation of the Balbiani body and consequent asymmetry of the ooplasm. These early nuclear and cytoplasmic asymmetries, however, are transient. During diplotene, the chromosome bouquet disappears, while the Balbiani body gradually disperses throughout the ooplasm. Finally, our observations indicate the presence of lampbrush chromosomes in the nuclei of previtellogenic oocytes. In the close vicinity to lampbrush chromosomes, characteristic spherical nuclear bodies are present.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Meiosis, Balbiani body and early asymmetry of Thermobia oocyte

et al. 2016

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Previtellogenic and vitellogenic oocytes in ovarian follicles of cultured siberian sturgeon Acipenser baerii (Chondrostei, Acipenseriformes)

Żelazowska

Fopp‐Bayat

2016

Journal of Morphology

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Previtellogenic and vitellogenic oocytes in ovarian follicles from cultured Siberian sturgeon Acipenser baerii were examined. In previtellogenic oocytes, granular and homogeneous zones in the cytoplasm (the ooplasm) are distinguished. Material of nuclear origin, rough endoplasmic reticulum, Golgi complexes, complexes of mitochondria with cement and round bodies are numerous in the granular ooplasm. In vitellogenic oocytes, the ooplasm comprises three zones: perinuclear area, endoplasm and periplasm. The endoplasm contains yolk platelets, lipid droplets, and aggregations of mitochondria and granules immersed in amorphous material. In the nucleoplasm, lampbrush chromosomes, nucleoli, and two types of nuclear bodies are present. The first type of nuclear bodies is initially composed of fibrillar threads only. Their ultrastructure subsequently changes and they contain threads and medium electron dense material. The second type of nuclear bodies is only composed of electron dense particles. All nuclear bodies impregnate with silver, stain with propidium iodide, and are DAPI-negative. Their possible role is discussed. All oocytes are surrounded by follicular cells and a basal lamina which is covered by thecal cells. Egg envelopes are not present in previtellogenic oocytes. In vitellogenic oocytes, the plasma membrane (the oolemma) is covered by three envelopes: vitelline envelope, chorion, and extrachorion. Vitelline envelope comprises four sublayers: filamentous layer, trabecular layer 2 (t2), homogeneous layer, and trabecular layer 1 (t1). In the chorion, porous layer 1 and porous layer 2 are distinguished in most voluminous examined oocytes. Three micropylar cells that are necessary for the formation of micropyles are present between follicular cells at the animal hemisphere. J. Morphol. 278:50-61, 2017. ©© 2016 Wiley Periodicals,Inc.

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Ovary micromorphology and oogenesis in a rhyacodriline oligochaete (Clitellata: Naididae, Rhyacodrilinae)

Świątek

Rodríguez

Małota

et al. 2022

Journal of Morphology

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The main goal of the article is to describe the ovary organization and oogenesis in Peristodrilus montanus, an aquatic oligochaete of the subfamily Rhyacodrilinae. The presented analysis will not only enrich the knowledge about how eggs are formed but, because of the suggested conservatism of ovary organization in clitellate annelids, can contribute to disentangling the complex phylogenetic relationships of the rhyacodrilines within Naididae. The paired, conically shaped ovaries are located in segment XI. They are composed of a dozen or so syncytial germ‐line cysts, which are associated with somatic cells. Each germ cell in a cyst has one intercellular bridge that joins it to a central and anuclear cytoplasmic mass, the cytophore. This pattern of cyst organization is typical for all clitellates that have been studied to date. Initially, the germ cells in a cyst undergo a synchronous development, however, there is no synchrony between cysts, and therefore there is a developmental gradient (oogonia, pre‐diplotene germ cells, germ cells in diplotene) of oogenesis along the long ovary axis. The cysts are composed of a maximum of 32 cells. Cysts with cells in diplotene detach from the ovaries and the extraovarian phase of oogenesis begins. The developmental synchrony is lost, one cell (an oocyte) per cyst starts to gather cell components and yolk and grows considerably. The remaining cells grow to some extent and function as nurse cells. Like in other microdriles, P. montanus oocytes are rich in yolk; other features of oogenesis are also similar to those that are known from other microdrile taxa. The system of ovary organization found in the studied species is broadly similar to the corresponding features known from Naidinae and Phreodrilidae and, to some extent, in Enchytraeidae. However, this system is different from the one that is known in Tubificinae, Limnodriloidinae and Branchiurinae.

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Morphology and ultrastructure of the germarium in panoistic ovarioles of a basal “apterygotous” insect, Thermobia domestica

Cited by 29 publications

References 38 publications

Meiosis, Balbiani body and early asymmetry of Thermobia oocyte

Meiosis, Balbiani body and early asymmetry of Thermobia oocyte

Previtellogenic and vitellogenic oocytes in ovarian follicles of cultured siberian sturgeon Acipenser baerii (Chondrostei, Acipenseriformes)

Ovary micromorphology and oogenesis in a rhyacodriline oligochaete (Clitellata: Naididae, Rhyacodrilinae)

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