Morphology and viability of pollen grains from passion fruit species (Passiflora spp.)

Soares, Taliane Leila; Jesus, O. N. de; Souza, Everton Hilo de; Santos-Serejo, Janay Almeida dos; Oliveira, E. J. de

doi:10.1590/s0102-33062013000400018

Cited by 16 publications

(12 citation statements)

References 28 publications

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“…The pollenkitt can also protect against dehydration and UV radiation damage, as well as with the pollen grain recognition system on the stigmatic surface (Pacini and Hesse 2005). Other authors also observed the presence of pollenkitt in the exine of the pollen grains of Passiflora (Souza et al 2004, Soares et al 2013, Silvério and Mariath 2014.…”

Section: Discussionmentioning

confidence: 94%

Comparative pollen morphological analysis in the subgenera Passiflora and Decaloba

Soares

Jesus

Souza

et al. 2018

An. Acad. Bras. Ciênc.

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The genus Passiflora is the most diversified of the Passifloraceae, and its palynology presents wide morphological variability. The objective of the study was to evaluate the pollen morphology of 18 Passiflora species in order to identify informative pollinic characteristics to contribute to the taxonomic classification of the genus. The morphology of the pollen grains and the exine structure were investigated using light microscopy and scanning electron microscopy. Differences in the pollen morphology were found in the studied species, mainly in terms of shape, pollen aperture and ornamentation pattern of the exine. Most of the species belonging to the subgenus Passiflora presented ornamented 6-syncolpate pollen grains with an oblate-spheroidal shape and an exine with large lumens. In the subgenus Decaloba the pollen grains were 6-colporate, 12-colpate and 12-colporate, with subprolate to prolate-spheroidal shape, as well as an exine with smaller lumen size and few ornamented. The pollen morphology of the species of the subgenera Passiflora and Decaloba has shown palynological characteristics that have specific diagnostic value, thus allowing a better understanding of the taxonomy of the genus Passiflora.

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Section: Discussionmentioning

confidence: 94%

Comparative pollen morphological analysis in the subgenera Passiflora and Decaloba

Soares

Jesus

Souza

et al. 2018

An. Acad. Bras. Ciênc.

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“…The assessment of viability using TTC is based on the color change of the tissues in the presence of a salt solution of 2,3,5-triphenyltetrazolium chloride, which is reduced by dehydrogenase respiratory enzymes in live tissues, resulting in a red carmine-colored compound called formazan (Beyhan and Serdan, 2008). The TTC test has been used to estimate the pollen viability in many species (Kelen and Demirtas, 2003;Huang et al, 2004;Kang et al, 2009;Soares et al, 2013). Several authors argued that the TTC test is a reliable estimative of pollen viability, showing similar results to in vitro germination tests (Bolat and Pirlak, 1999;Huang et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

“…Each species requires a specific protocol of culture medium to obtain adequate pollen grain germination (Soares et al, 2013). The pH of the culture medium considered ideal for in vitro Means followed by the same lower-case letters in the column and upper-case letters in the row within the same factor do not differ from each other by the Tukey test at 5% probability.…”

Section: Discussionmentioning

confidence: 99%

Effect of collection time on the viability of banana pollen grains

Soares¹,

Souza²,

Sampaio³

et al. 2015

Afr. J. Biotechnol.

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The objective of this study was to assess the pollen viability of six improved diploid banana plants (AA) collected in different periods of the day, in two seasons of the year (winter and summer), using in vitro germination test and staining. Pollen grains collected at 8 am (anthesis), 10 am, 12 pm, 2 pm and 4 pm were evaluated. We used a culture medium for pollen germination containing 15% sucrose, 0.01% H 3 BO 3 , 0.01% KNO 3 , 0.03% Ca(NO 3 ) 2 .4H 2 O, and 0.02% MgSO 4 .7H 2 O, solidified with 0.8% agar, adjusted to different pH. The pollen viability was evaluated by staining with 2,3,5-triphenyltetrazolium chloride (TTC). The highest pollen germination rates and viability were obtained at 8 am and the lowest at 4 pm, in both seasons. The average in vitro germination percentage and viability level were negatively influenced by the number of hours after anthesis. During the summer, the pollen viability and in vitro germination rates were highest when compared to winter period. The results presented can help at the selection of genetic materials and enable inferences on the best pollen collection time for use in cross breeding programs for plant improvement.

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“…TTC staining method is one of the widely and fast used methods based on dehydrogenase enzymatic reaction in mitochondria of living cells (Firmage & Dafni 2001, Soares et al 2013.…”

Section: Introductionmentioning

confidence: 99%

Effect of vehicular traffic on pollen size and viability of Apocynaceous plant species

Kaur¹,

Nagpal²

2017

Trop. Plant Res.

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Vehicular traffic adds up different kinds of contaminants like heavy metals, polyaromatic hydrocarbons, gaseous toxins etc. to the atmosphere. Pollen grains of different plants have been found to be sensitive to these pollutants, which show varied effects on their viability, and size and hence can be used as bioindicators of air pollution. Keeping this in mind, the present work was designed to measure the pollen viability and pollen size of three common roadside plants, namely Alstonia scholaris, Nerium oleander and Tabernaemontana divaricata belonging to Apocynaceae family growing at seven sites of Amritsar city with varying levels of traffic stress. Pollen viability and pollen size were studied using 2, 3, 5-triphenyl tetrazolium chloride (TTC) staining method. The percentage of pollen viability of A. scholaris ranged from 32.45% to 65.74%, for N. oleander it ranged from 30.3% to 45.11% and for T. divaricata it ranged from 14.31% to 49.62%. Pollen size ranged from 22.86 to 23.94 µm for A. scholaris, 29.58 to 32.17 µm for N. oleander and 29.83-32.43 µm for T. divaricata. Pollen viability was found to be inversely proportional to a load of traffic at a given site whereas no remarkable variation was found in pollen size. For A. scholaris, a significant negative correlation (r= -0.762, p≤0.05) was observed between pollen viability and traffic load, while for other two species correlation between pollen viability and traffic load was not statistically significant at p≤0.05.

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Morphology and viability of pollen grains from passion fruit species (Passiflora spp.)

Cited by 16 publications

References 28 publications

Comparative pollen morphological analysis in the subgenera Passiflora and Decaloba

Comparative pollen morphological analysis in the subgenera Passiflora and Decaloba

Effect of collection time on the viability of banana pollen grains

Effect of vehicular traffic on pollen size and viability of Apocynaceous plant species

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