Morphology effects on singlet oxygen production and bacterial photoinactivation efficiency by different silica-protoporphyrin IX nanocomposites

Zampini, Giulia; Planas, Oriol; Marmottini, Fabio; Gulías, Òscar; Agut, Montserrat; Nonell, Santi; Latterini, Loredana

doi:10.1039/c7ra00784a

Cited by 24 publications

(13 citation statements)

References 55 publications

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“…It is worth recalling that recently it has been reported that samples similar to those under investigation are photosensitizing the formation of singlet oxygen, which has been quantified by directly monitoring its phosphorescence . These data support the hypothesis that even in the present experimental conditions the samples sensitize the formation of single oxygen; since an indirect method of monitoring ROS formation has been used in the present work, for correctness we will continue to refer generally to reactive oxygen species.…”

Section: Resultssupporting

confidence: 84%

“…It has been recently reported that PpIX can be chemically adsorbed on mesoporous silica nanoparticles to minimize the aggregation and assist singlet‐oxygen delivery in aqueous media . Indeed, the morphology and size of pores on the silica surface have been found beneficial for the distribution of PpIX in its monomeric form . However, the porosity of the nanoparticles plays a pivotal role on the singlet‐oxygen production but also on its release from the nanoparticles and delivery to the target cells.…”

Section: Introductionmentioning

confidence: 99%

“…However, the porosity of the nanoparticles plays a pivotal role on the singlet‐oxygen production but also on its release from the nanoparticles and delivery to the target cells. In particular, compact regular silica nanoparticles, with PpIX anchored exclusively on the surface, presented the highest biological activity, despite the lower efficiency in singlet‐oxygen formation, whereas large‐pore mesoporous silica nanoparticles, which had the highest singlet‐oxygen photosensitization yield, displayed lower inactivation efficiency . This behaviour has been related to chemical properties of the particle surface (zeta potential) and/or to the ability of singlet oxygen to escape from the pores where it formed.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Gold Colloids on the Photosensitization Efficiency of Silica Particles Doped with Protoporphyrin IX

et al. 2017

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Photodynamic treatments represent a non‐invasive tool to intervene in the cell cycle, only when needed, but high excitation power is an important limitation. In this work, protoporphyrin IX (PpIX) is covalently anchored to the surface of silica particles. The hybrid colloids are prepared with different photosensitizer loadings and characterized by TEM, SEM‐EDX and spectroscopic methods. The visible excitation of the colloidal samples generates reactive oxygen species, which are quantified by use of a specific chemical scavenger agent (1,3‐diphenylisobenzofuran). The determined efficiencies depend on the PpIX loading, being higher in the sample where the aggregation is reduced. To enhance further the photosensitizing performance of silica‐PpIX samples, increasing amounts of gold nanoparticles are conjugated to the silica surface in order to exploit the antenna effect of plasmonic nanostructures. The visible excitation of silica‐PpIX systems in the presence of gold nanoparticles results in a faster and more efficient photoinduced formation of ROS species reaching a 230 % enhancement of ROS photosensitization when 1013 Au nanoparticles mL−1 (corresponding to circa 75 ppm of gold) are present. The efficiency data are discussed in terms of plasmonic enhancement of the excitation field.

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Section: Resultssupporting

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of Gold Colloids on the Photosensitization Efficiency of Silica Particles Doped with Protoporphyrin IX

et al. 2017

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“…Under dark conditions, porphyrins do not exhibit significant cyto‐ or genotoxicity (Donnelly et al ). Until now applications for porphyrins were essentially performed on cancer cells for photodynamic therapy or on microorganisms as antimicrobial agents (Pläetzer et al , Maish , Pérez‐Lagunal et al , Zampini et al ). For example, upon illumination, protoporphyrin IX and other derivatives induced a strong cytotoxic effect in yeasts Saccharomyces cerevisiae and Candida albicans (Carré et al , Donnelly et al , Cormick et al , Funes et al , Quiroga et al ).…”

Section: Introductionmentioning

confidence: 99%

Responses of an adventitious fast‐growing plant to photodynamic stress: comparative study of anionic and cationic porphyrin effect on Arabidopsis thaliana

Issawi

Guillaumot

Sol

et al. 2017

Physiologia Plantarum

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Antimicrobial photodynamic treatment (APDT) based on the use of a photosensitizer to produce reactive oxygen species (ROS) that induce cell death could be envisaged to fight against plant pathogens. For setting this strategy, we want to study how plants themselves respond to photodynamic treatment. In previous work we showed that tomato plantlets were able to resist photoactivated tetra (N-methylpyridyl) porphyrin (CP) or the zinc metalated form (CP-Zn). To enlarge our plant expertise related to exogenous porphyrins treatment and to further defend this approach, we studied how a weed like Arabidopsis thaliana responded to exogenous supply of anionic and cationic porphyrins. Both types of photosensitizers had no negative effect on seed germination and did not hamper the development etiolated Arabidopsis plantlet under dark conditions. Thus, post-emergence effects of porphyrin photoactivation on the development of 14 day-old in vitro Arabidopsis plantlet under light were observed. CP-Zn was the most efficient photosensitizer to kill Arabidopsis plantlets while anionic tetra (4-sulfonatophenyl) porphyrin only delayed their growth and development. Indeed only 7% of plantlets could be rescued after CP-Zn treatment. Furthermore, non-enzymatic and enzymatic defense components involved in detoxification of ROS generated by CP-Zn under illumination were downregulated or stable with the exception of sevenfold increase in proline content. As previously demonstrated in the literature for microbial agents and in the present work for Arabidopsis, CP-Zn was efficient enough to eradicate unwanted vegetation and plant pathogens without at the same time killing plants of agronomic interest such as tomato plantlets.

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“…Latterini and co‐workers prepared different silica‐protoporphyrin IX (PpIX) derivatives as antimicrobial agents . The systems—compact silica nanoparticles (CSNP), mesoporous silica nanoparticles (MPSAm), stellate mesoporous silica nanoparticles (MPSSt), and larger pore mesoporous silica nanoparticles (MPSLP)—functionalized with PpIX were synthesized to evaluate the morphology‐dependence of the nanomaterials in the singlet oxygen production and their bacterial efficiency against S. aureus .…”

Section: Ms In Antibacterial Applicationsmentioning

confidence: 99%

Mesoporous Silica‐Based Materials with Bactericidal Properties

Bernardos

Piacenza

Sancenón

et al. 2019

Small

146

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Bacterial infections are the main cause of chronic infections and even mortality. In fact, due to extensive use of antibiotics and, then, emergence of antibiotic resistance, treatment of such infections by conventional antibiotics has become a major concern worldwide. One of the promising strategies to treat infection diseases is the use of nanomaterials. Among them, mesoporous silica materials (MSMs) have attracted burgeoning attention due to high surface area, tunable pore/particle size, and easy surface functionalization. This review discusses how one can exploit capacities of MSMs to design and fabricate multifunctional/controllable drug delivery systems (DDSs) to combat bacterial infections. At first, the emergency of bacterial and biofilm resistance toward conventional antimicrobials is described and then how nanoparticles exert their toxic effects upon pathogenic cells is discussed. Next, the main aspects of MSMs (e.g., physicochemical properties, multifunctionality, and biosafety) which one should consider in the design of MSM‐based DDSs against bacterial infections are introduced. Finally, a comprehensive analysis of all the papers published dealing with the use of MSMs for delivery of antibacterial chemicals (antimicrobial agents functionalized/adsorbed on mesoporous silica (MS), MS‐loaded with antimicrobial agents, gated MS‐loaded with antimicrobial agents, MS with metal‐based nanoparticles, and MS‐loaded with metal ions) is provided.

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Morphology effects on singlet oxygen production and bacterial photoinactivation efficiency by different silica-protoporphyrin IX nanocomposites

Abstract: Different silica-protoporphyrin IX (PpIX) nanocomposites have been synthesized to evaluate the dependence of singlet oxygen production and bacterial inactivation efficiency on the morphology of the nanomaterials.

Cited by 24 publications

References 55 publications

Effects of Gold Colloids on the Photosensitization Efficiency of Silica Particles Doped with Protoporphyrin IX

Effects of Gold Colloids on the Photosensitization Efficiency of Silica Particles Doped with Protoporphyrin IX

Responses of an adventitious fast‐growing plant to photodynamic stress: comparative study of anionic and cationic porphyrin effect on Arabidopsis thaliana

Mesoporous Silica‐Based Materials with Bactericidal Properties

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