Morphology, molecular profiles and distribution of Japanese populations of Steinernema tielingense Ma, Chen, Li, Han, Khatri-Chhetri, De Clercq & Moens, 2012 (Rhabditida: Steinernematidae)

Abstract

Summary Steinernema tielingense was found to be widely distributed in Japan. The molecular characteristics of the nematodes isolated from Japan are basically in accordance with those mentioned in the original description of S. tielingense. However, the Japanese isolates are morphologically distinct from the type population by the following characters: length of the infective juvenile, 711 (671-769) μm, excretory pore of adult males and infective juveniles located posterior mid-pharynx, D% < 50%, smaller H value of 35% and shorter spicules in adult males of both generations. Although discrepancies in morphology and morphometric data between the Chinese isolate and Japanese isolate were observed, the molecular data matched and on this basis the Japanese isolate was identified as being conspecific with S. tielingense. The symbiotic bacterium isolated from the Japanese population of S. tielingense was identified as Xenorhabdus bovienii.

Systematics and phylogeny of the entomopathogenic nematobacterial complexes Steinernema–Xenorhabdus and Heterorhabditis–Photorhabdus

“…close to X. bovienii * [ 102 , 163 ] S. puntauvense X. bovienii subsp. bovienii [ 102 , 163 ] S. sandneri - S. sangi X. thuongxuanensis [ 99 ] and X. vietnamensis [ 95 ] S. silvaticum X. bovienii * [ 164 ] S. texanum - S. tielingense X. bovienii * [ 165 ] S. weiseri X. bovienii subsp. bovienii [ 94 , 102 ] S. xinbinense X. aichiensis** S. xueshanense Xenorhabdus sp.…”