Morphology, physicochemical properties and antioxidant capacity of bee pollens

Bleha, Roman; Shevtsova, Tetiana; Sinica, Andrej; Kružík, Vojtěch; Brindza, Ján

doi:10.17221/139/2018-cjfs

Cited by 16 publications

(14 citation statements)

References 39 publications

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“…The FTIR-ATR spectra of pollens belonging to different genera as shown in Figure 2 were only a sample representative of each genus reported. Main constituents of bee pollen are proteins, saccharides and lipids; IR spectra of the pollen samples have similar characteristic peaks [17,18,19], but several peculiarities can be found in dissimilar pollens due to little differences in chemical compositions. The meaningful IR peaks of bee pollens are shown in Table 3.…”

Section: Resultsmentioning

confidence: 99%

“…Kasprzyk et al in 2018 [18] investigated the floral origin of bee pollens using a sequence of chemometric procedures (DA, HCA, etc.) applied on parameters obtained from FTIR spectra to confirm the floral origin of rape pollen; Svecniak et al in 2015 [20] explored the possible clustering of unifloral honeys belonging to different pollens using principal component analysis; in addition Bleha et al in 2019 [17] characterized six unifloral bee pollens of various botanical origins by morphometry, SEM, CIE L*a*b* color parameters and FTIR spectroscopy. However, in the literature there are no reports on the use of FTIR data together with those of color and granulometry to identify the botanical origin of bee pollen loads.…”

Section: Discussionmentioning

confidence: 99%

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Morphological, Physicochemical and FTIR Spectroscopic Properties of Bee Pollen Loads from Different Botanical Origin

et al. 2019

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Bee pollen loads generally have a homogeneous and monospecific pollen content and assume a typical form and color, due to the typical bee foraging habits, thus having a typical composition related to the botanical origin. The present study aims to characterize bee pollen loads belonging to different botanical species using morphological, spectroscopic and color properties and to find relationships between these variables. IR spectra analysis allowed to have a reliable picture of the components present in the different samples; color and granulometry permits a visual identification of pollen load belonging to different species. Multivariate analysis enabled differentiation among the botanical origin of most of the bee pollen samples, grouping them according to the family and the genus and confirming the possibility to use IR and color measurements for the evaluative analysis and classification of bee pollen samples, to promote the consumption of this bee product as functional food.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Morphological, Physicochemical and FTIR Spectroscopic Properties of Bee Pollen Loads from Different Botanical Origin

et al. 2019

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“…In addition, statistical evaluation of the image spectra may be useful for the evaluation of bee pollen homogeneity and for the discrimination of bee pollen samples yielding trends and clusters [8,16]. In our previous works [8,9,18,19], vibrational spectroscopy and color analysis based on diffuse reflectance VIS spectra were used in the analysis of homogenates of bee pollen and bee bread; however, this approach is not able to evaluate the botanical and color heterogeneity of these beekeeping products.…”

Section: Introductionmentioning

confidence: 99%

Spectroscopic Discrimination of Bee Pollen by Composition, Color, and Botanical Origin

Bleha

Shevtsova

Živčáková

et al. 2021

Foods

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Bee pollen samples were discriminated using vibrational spectroscopic methods by connecting with botanical sources, composition, and color. SEM and light microscope images of bee pollen loads were obtained and used to assess the botanical origin. Fourier transform (FT) mid- and near-infrared (FT-MIR, FT-NIR), and FT-Raman spectra of bee pollen samples (a set of randomly chosen loads can be defined as an independent sample) were measured and processed by principal component analysis (PCA). The CIE L*a*b* color space parameters were calculated from the image analysis. FT-MIR, FT-NIR, and FT-Raman spectra showed marked sensitivity to bee pollen composition. In addition, FT-Raman spectra indicated plant pigments as chemical markers of botanical origin. Furthermore, the fractionation of bee pollen was also performed, and composition of the fractions was characterized as well. The combination of imaging, spectroscopic, and statistical methods is a potent tool for bee pollen discrimination and thus may evaluate the quality and composition of this bee-keeping product.

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“…Alicic et al 16 and Leja et al 11 reported the action mechanism responsible for antioxidant and antibacterial potential of pollen grains that accompanying with the existence of flavonoids, phenolic acids, and pigments like as β-carotene. Lately, Bleha et al 17 evidenced the antioxidant potential of Bee pollen extracts correlated with high content of polyphenols. The high contents of flavonoids and phenols of Apis mellifera pollen grains represent the main reasons antioxidant activity 18.…”

Section: Introductionmentioning

confidence: 99%

Genotoxic and Antitumor Activity of Pollen Grains against Prostate Cancer Cell Line

Ganash¹

2021

Int J Pharma Bio Sci

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Since the use of engineered antioxidants and antitumor is under investigation, inferable from its likely poisonousness, scientists have deflected their thoughtfulness regarding the quest for characteristic sources to meet the human medication and diet requests. Therefore the study aimed to evaluate the antitumor and antioxidant activities of maize pollen grains against the Prostate Cancer Cell (Pc3) line. Maize pollen grains were collected by Bee through a pollen trap, and then subjected for flavonoids and alkaloids analysis by HPLC method. an in vitro assays, were used to test the antitumor properties, against Pc3 cells. Furthermore, its antioxidant potential was also evaluated by DPPH. The detected flavonoids were identified to be quercetin, luteolin kaempferol, rutin, apigenin and naringin and the alkaloids were quinolone, hydroxyindolenine and conofoline. The antitumor efficacy of pollen grains extract increased with concentration and reached to 94.92 % that similar to the toxicity % of adriamycin at 1000 µg/mL, however, the IC50 (339.81 µg) of pollen grains extract was highest than IC50 (58.07 µg) of adriamycin. At 500 μg/mL of pollen grains extract, morphological changes of Pc3 were recorded. These changes deformed more at 1000 μg/mL. DPPH scavenging activity was found to be 92.26 % at 1280 µg/mL of pollen grains extracted with IC50 425.4 µg/mL compared with IC50 (13.9 µg/mL) of the ascorbic acid. DNA fragmentation and quantitative RT-PCR examinations of Bax and Bcl-2 genes demonstrated that pollen grains extract induced cellular apoptosis of Pc3 cells. This study concluded that the maize pollen grains may applied as natural safe source for inhibit Pc3 Cells proliferation as well as applied as antioxidant.

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Morphology, physicochemical properties and antioxidant capacity of bee pollens

Cited by 16 publications

References 39 publications

Morphological, Physicochemical and FTIR Spectroscopic Properties of Bee Pollen Loads from Different Botanical Origin

Morphological, Physicochemical and FTIR Spectroscopic Properties of Bee Pollen Loads from Different Botanical Origin

Spectroscopic Discrimination of Bee Pollen by Composition, Color, and Botanical Origin

Genotoxic and Antitumor Activity of Pollen Grains against Prostate Cancer Cell Line

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