Morphology, toxin composition and pigment content of Prorocentrum lima strains isolated from a coastal lagoon in southern UK

Nascimento, Silvia M.; Purdie, Duncan A.; Morris, Steven

doi:10.1016/j.toxicon.2004.12.023

Cited by 85 publications

(109 citation statements)

References 34 publications

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“…Some of them are potential toxin producers (Nakajima et al 1981). Epiphytic Prorocentrum species are mainly associated with okadaic acid and the production of analogues (Faust 1991, Nascimento et al 2005. These findings support those of Romdhane et al (1998) and Armi et al (2010) in Tunisia and those of Aligizaki and Nikolaidis (2006), Mangialajo et al (2008) and Totti et al (2010) in the northern Mediterranean Sea.…”

Section: Discussionsupporting

confidence: 85%

Variability in the structure of epiphytic microalgae assemblages on the leaves of <em>Posidonia oceanica</em> in relation to human disturbance in a meadow off Tunisia

et al. 2014

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Summary:We investigated the impact of sewage discharge on Posidonia oceanica meadows in Mahdia, eastern Tunisia. We specifically addressed changes in biometric plant parameters and epiphytic microalgae composition on leaves caused by this anthropogenic interference. A hierarchical sampling design was used to compare epiphytic microalgae structure between one disturbed and two control stations. Samples were collected by SCUBA diving at 8 m depth in August 2009. A total of 58 microalgae taxa were identified on leaves. At the disturbed station, leaf length, leaf surface area and the leaf area index decreased, whereas epiphytic dinoflagellate abundances increased compared with the control stations, with more Prorocentrales, Protoperidiniales and potentially toxic species on leaves of the disturbed station. Moderate nutrient enrichment (mainly Si(OH) 4 and NH 4 + ) and low water transparency at the disturbed station were associated with the increased abundances of some dinoflagellate species.Keywords: epiphytic microalgae; sewage disturbance; spatial variability; Posidonia oceanica.Variabilidad en la estructura de las comunidades de microalgas epífitas en las hojas de Posidonia oceanica en respuesta a las perturbaciones humanas en una pradera de Túnez Resumen: Se ha investigado el impacto de los vertidos de aguas residuales en praderas de Posidonia oceanica situadas en Mahdia (Este de Túnez). Se han estudiado los cambios en los parámetros biométricos de la planta y la composición de las microalgas epífitas en las hojas causadas por esta interferencia antrópica. Se ha utilizado un diseño de muestreo jerárquico para comparar las microalgas epífitas entre la estación perturbada y dos estaciones control. Las muestras se recolectaron en buceo autónomo a 8 m de profundidad en Agosto de 2009. La longitud, el área y el índice de área de las hojas era inferior en la estación perturbada mientras que las abundancias de los dinoflagelados epífitos eran mayores en las estaciones control, con más Prorocentrales, Protoperidiniales y otras especies tóxicas potenciales en las hojas de la estación perturbada. Un ligero aumento de nutrientes en la estación perturbada (sobre todo Si(OH) 4 y NH 4 + ) y una menor transparencia del agua iba asociada a un aumento en la abundancia de algunas especies de dinoflageladas.

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Section: Discussionsupporting

confidence: 85%

Variability in the structure of epiphytic microalgae assemblages on the leaves of <em>Posidonia oceanica</em> in relation to human disturbance in a meadow off Tunisia

et al. 2014

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“…This is despite the fact that Dinophysiales and Prorocentrales share major synapomorphies. Both lineages have a theca divided into lateral halves and have two apical pores, one large and the other small, with both flagella arising from the larger pore (39), and many Dinophysis and some Prorocentrum species (e.g., P. lima) produce polyether-type toxins (27). The apparently close relationship between the two dinoflagellate orders based on these features is not strongly supported by phylogenies based on multiple genes and editing characteristics.…”

Section: Discussionmentioning

confidence: 99%

Mitochondrial cob and cox1 Genes and Editing of the Corresponding mRNAs in Dinophysis acuminata from Narragansett Bay, with Special Reference to the Phylogenetic Position of the Genus Dinophysis

Zhang

Bhattacharya

Maranda

et al. 2008

Appl Environ Microbiol

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Dinophysis acuminata cells were isolated from Narragansett Bay water samples in June 2005 using flow cytometry. Dinoflagellate-specific PCR primers were used to isolate small-subunit rRNA (18S rRNA), mitochondrial cytochrome b (cob), and cytochrome c oxidase I (cox1) genes and the encoded cDNAs. Maximumlikelihood analysis of a concatenated data set of ribosomal DNA and cDNA sequences of cob and cox1 showed that D. acuminata was sister to Gonyaulacoids, but without strong bootstrap support. The approximately unbiased test could not reject alternative positions of D. acuminata. To gain better resolution, mRNA editing of cob and cox1 was inferred for D. acuminata and 13 other dinoflagellate species. The location and type of editing as well as the distribution pattern in D. acuminata were generally similar to those in other dinoflagellates except for two edited sites that are unique to this species. Bayesian analyses of a matrix that recorded the location and type of editing, and of a matrix that included the protein sequences of COB and COX1 with the editing data yielded tree topologies similar to the three-gene tree but again failed to resolve the phylogenetic position of D. acuminata. However, the density of edited sites in the D. acuminata mitochondrial genes, consistent with phylogenetic trees, indicated that Dinophysis is a derived dinoflagellate lineage, diverging after other lineages such as Oxyrrhis, Amphidinium, and Symbiodinium. We demonstrate that dinoflagellate-specific PCR coupled with flow cytometry can be a useful tool to analyze genes and their transcripts from a natural dinoflagellate population.

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“…Nascimento et al, 2005;Mohammad-Noor et al, 2006;Aligizaki et al, 2009). Great variability in cell size and shape was observed by Aligizaki et al (2009) for P. lima strains from the North Aegean coastal waters, who considered them to be members of a P. lima species complex.…”

Section: Taxonomic Remarksmentioning

confidence: 99%

“…Outside tropical and subtropical areas, Ostreopsis is very frequent and well-documented in the Mediterranean Sea (Vila et al, 2001;Turki, 2005;Aligizaki & Nikolaidis, 2006;Ciminiello et al, 2006;Mangialajo et al, 2008;Totti et al, 2010) and reports of blooms in temperate waters have increased in recent years (Shears & Ross, 2009;Rhodes, 2010). Coolia and Prorocentrum appear to be more widely distributed, extending to colder waters than Gambierdiscus and Ostreopsis (Faust, 1991;Bravo et al, 2001;Levasseur et al, 2003;Nascimento et al, 2005;Maranda et al, 2007;Vale et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Morphological and genetic characterization of benthic dinoflagellates of the generaCoolia,OstreopsisandProrocentrumfrom the south-eastern Bay of Biscay

Laza‐Martínez

Orive

Miguel

2011

European Journal of Phycology

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Benthic dinoflagellates of the genera Coolia, Ostreopsis and Prorocentrum isolated from coastal waters of the south-eastern Bay of Biscay were identified morphologically by means of light microscopy (LM) including epifluorescence microscopy, and scanning electron microscopy (SEM). To identify the strains to species level, molecular phylogenetic analyses using the nuclear large subunit rDNA (LSU) were performed for 16 strains of the three genera. These morphological and phylogenetic analyses revealed the presence of the following species: Coolia canariensis S. Fraga, Coolia monotis Meunier, Ostreopsis cf. siamensis Schmidt, Prorocentrum emarginatun Fukuyo, P. lima (Ehrenberg) Dodge, P. rhathymum Loeblich III, Sherley & Schmidt, and two as yet unidentified species, which in the phylogenetic tree were grouped with different strains of Prorocentrum emarginatun and P. fukuyoi Murray et Nagahama from GenBank. A strain from Minorca (Balearic Islands, western Mediterranean Sea) analysed in this study for comparative purposes and fitting morphologically into the P. emarginatum/P. fukuyoi group also appeared in this cluster, which seems to include morphologically cryptic or semicryptic species. The most common taxa were Coolia monotis, Ostreopsis cf. siamensis and Prorocentrum lima, which appeared at most sampling sites. Only the strains corresponding to Ostreopsis cf. siamensis and Prorocentrum lima were toxic to Artemia franciscana.

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Morphology, toxin composition and pigment content of Prorocentrum lima strains isolated from a coastal lagoon in southern UK

Cited by 85 publications

References 34 publications

Variability in the structure of epiphytic microalgae assemblages on the leaves of <em>Posidonia oceanica</em> in relation to human disturbance in a meadow off Tunisia

Variability in the structure of epiphytic microalgae assemblages on the leaves of <em>Posidonia oceanica</em> in relation to human disturbance in a meadow off Tunisia

Mitochondrial cob and cox1 Genes and Editing of the Corresponding mRNAs in Dinophysis acuminata from Narragansett Bay, with Special Reference to the Phylogenetic Position of the Genus Dinophysis

Morphological and genetic characterization of benthic dinoflagellates of the generaCoolia,OstreopsisandProrocentrumfrom the south-eastern Bay of Biscay

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