Morphometric Analysis of Human Bone Biopsies: A Quantitative Structural Comparison of Histological Sections and Micro-Computed Tomography

Müller, Ralph; Campenhout, H Van; Damme, B Van; Perre, Georges Van der; Dequeker, Jan; Hildebrand, T.; Rüegsegger, P.

doi:10.1016/s8756-3282(98)00068-4

Cited by 587 publications

(455 citation statements)

References 22 publications

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“…Micro-CT is a relatively recent, non-destructive high-resolution imaging technique, validated by 2D histology and physical 3D phantoms [18,25,26,28], capable of 2D and 3D quantitative characterisation of excised bone segments at a detection level not achievable with conventional MRI or CT scanners. It has a spatial resolution (10 lm range), which is 10-100 times better than conventional clinical CT or MRI scanners, and can accurately differentiate between the trabecular and cortical bone compartments and non-osseous tissues, as opposed to conventional clinical CT and MRI imaging [14,26,27,29]. Although it has been used in spine studies previously, it has never been used to study in Modic changes before [30][31][32].…”

Section: Discussionmentioning

confidence: 99%

“…Although it has been used in spine studies previously, it has never been used to study in Modic changes before [30][31][32]. It is typically used for ex vivo scanning of excised human and animal bones [14,29] or in vivo scanning of small animals, at micrometer resolution [33]. For clinical CT scans on human patients in vivo, the improvement in spatial resolution (decrease of pixel size) is currently limited by the corresponding increase in radiation dose [28].…”

Section: Discussionmentioning

confidence: 99%

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Modic (endplate) changes in the lumbar spine: bone micro-architecture and remodelling

et al. 2014

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Purpose In the literature, inter-vertebral MRI signal intensity changes (Modic changes) were associated with corresponding histological observations on endplate biopsies. However, tissue-level studies were limited. No quantitative histomorphometric study on bone biopsies has yet been conducted for Modic changes. The aim of this study was to characterise the bone micro-architectural parameters and bone remodelling indices associated with Modic changes. Methods Forty patients suffering from disabling low back pain, undergoing elective spinal surgery, and exhibiting Modic changes on MRI (Modic 1, n = 9; Modic 2, n = 25; Modic 3, n = 6), had a transpedicular vertebral body biopsy taken of subchondral bone. Biopsies were first examined by micro-CT, for 3D morphometric analysis of bone volume fraction (BV/TV), trabecular thickness (Tb.Th), trabecular separation, trabecular number, and structure model index. Then, samples underwent histological analysis, for determination of bone remodelling indices: osteoid surface to bone surface ratio (OS/BS), eroded surface to bone surface (ES/BS) and osteoid surface to eroded surface ratio (OS/ES). Results Micro-CT analysis revealed significantly higher BV/TV (up to 70 % increase, p \ 0.01) and Tb.Th (up to ?57 %, p \ 0.01) in Modic 3 biopsies, compared to Modic 1 and 2. Histological analysis showed significantly lower OS/BS in Modic 2 biopsies (more than 28 % decrease, p \ 0.05) compared to 1 and 3. ES/BS progressively decreased from Modic 1 to 2 to 3, whereas OS/ES progressively increased with significantly higher values in Modic 3 (up to 159 % increase, p \ 0.05) than in Modic 1 and 2. Conclusions Significant differences were found in bone micro-architectural parameters and remodelling indices among Modic types. Modic 1 biopsies had evidence of highest bone turnover, possibly due to an inflammatory process; Modic 2 biopsies were consistent with a reduced bone formation/remodelling stage; Modic 3 biopsies suggested a more stable sclerotic phase, with significantly increased BV/TV and Tb.Th compared to Modic 1 and 2, linked to increased bone formation and reduced resorption.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Modic (endplate) changes in the lumbar spine: bone micro-architecture and remodelling

et al. 2014

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“…33 Bone volume fraction (BV/TV) was obtained by dividing bone volume (BV) with the total volume (TV) representing apparent bone density. 34 The structure model index (SMI) defines the predominant shape of bony trabeculae: 0 represents perfect plates while 3 stands for ideal rods. 35 The degree of anisotropy (DA) describes the prevailing orientation of the trabeculae.…”

Section: Methodsmentioning

confidence: 99%

Microstructural adaptation in trapezial bone due to subluxation of the thumb

Nufer

Goldhahn

Köhler

et al. 2007

Journal Orthopaedic Research

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Although the thumb saddle is one of the most common sites of degenerative osteoarthritis in the hand, little is known about the altered microstructure in osteoarthritic trapezial bones. External forces resulting from subluxation of the carpometacarpal joint of the thumb (CMC I) should provoke microstructural changes in the trapezium. The purpose of this study was to compare the regional differences of the microstructure between osteoarthritic and healthy trapezial bones. Fifteen trapezia harvested from female patients with radiologically and clinically diagnosed saddle joint osteoarthritis (OA) were compared with 15 unaffected controls. Microstructural parameters, such as bone volume ratio (BV/TV), three-dimensional connectivity (Conn.D), trabecular number (Tb.N), and trabecular thickness (Tb.Th) were studied using a microcomputed tomography (microCT) system. While the trapezial height in OA was 22% less, the sclerotic subchondral bone layer thickness was 50% higher in OA compared with the control group (p < 0.001). In the OA group there was a 42% higher bone volume ratio (p 0.001), an 18% increase in Tb.Th (p ¼ 0.006), and a 10% greater Tb.N (p ¼ 0.034) compared with the control group. Although in both groups BV/TV was significantly lower in the radial region, the radial column showed the highest relative increase in bone volume and structure compared with the control group (þ67% BV/TV, þ20% Tb.Th, þ23% Tb.N). The reinforcement of the bony microstructure in CMC I OA, especially at the radial side, is a sign for bone adaptation reacting to radially shifted joint forces. This has to be considered during the development of new prosthetic alternatives. ß

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“…49 In addition to the visual assessment of structural images, morphometric indices were determined on the basis of microtomographic data sets by performing direct three-dimensional morphometry. 50,51 …”

Section: Evaluation Of Bone Formation In Vivomentioning

confidence: 99%

Ultrasound-based nonviral gene delivery induces bone formation in vivo

et al. 2007

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Nonviral gene delivery is a promising, safe, therapeutic tool in regenerative medicine. This study is the first to achieve nonviral, ultrasound-based, osteogenic gene delivery that leads to bone tissue formation, in vivo. We hypothesized that direct in vivo sonoporation of naked DNA encoding for the osteogenic gene, recombinant human bone morphogenetic protein-9 (rhBMP-9) would induce bone formation. A luciferase plasmid (Luc), encoding rhBMP-9 or empty pcDNA3 vector mixed with microbubbles, was injected into the thigh muscles of mice. After injection, noninvasive sonoporation was applied. Luc activity was monitored noninvasively, and quantitatively using bioluminescence imaging in vivo, and found for 14 days with a peak expression on day 7. To examine osteogenesis in vivo, rhBMP-9 plasmid was sonoporated into the thigh muscles of transgenic mice that express the Luc gene under the control of a human osteocalcin promoter. Following rhBMP-9 sonoporation, osteocalcin-dependent Luc expression lasted for 24 days and peaked on day 10. Bone tissue was formed in the site of rhBMP-9 delivery, as was shown by microcomputerized tomography and histology. The sonoporation method was also compared with previously developed electrotransfer-based gene delivery and was found significantly inferior in its efficiency of gene delivery. We conclude that ultrasound-mediated osteogenic gene delivery could serve as a therapeutic solution in conditions requiring bone tissue regeneration after further development that will increase the transfection efficiency.

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Morphometric Analysis of Human Bone Biopsies: A Quantitative Structural Comparison of Histological Sections and Micro-Computed Tomography

Cited by 587 publications

References 22 publications

Modic (endplate) changes in the lumbar spine: bone micro-architecture and remodelling

Modic (endplate) changes in the lumbar spine: bone micro-architecture and remodelling

Microstructural adaptation in trapezial bone due to subluxation of the thumb

Ultrasound-based nonviral gene delivery induces bone formation in vivo

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