Morphometric analysis of the deposition and mineralization of enamel and dentine from rat incisor during the recovery phase following a low-calcium regimen

Lozupone, E; Favia, A

doi:10.1016/0003-9969(94)90171-6

Cited by 26 publications

(14 citation statements)

References 22 publications

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“…Moreover, previous results [31,32] suggest that the reduction in the growth of the rat, caused by calcium deficiency, could be balanced by the addition of calcium to their diet, which leads to recovery of the growth rate of the animals and restores the dentinogenesis via odontoblast activity to its genetically determined level. It is also noticeable that experimental diets of this study were slightly below the recommended energy level (18.42 kJ/g), but no deprivation of energy occurred because rats increase their food intake to meet their energy demands [33].…”

Section: Discussionmentioning

confidence: 96%

The Reducing Effects of a Calcium-Deficient Diet and High Sucrose Diet on Dentin Apposition of Rat Molars

et al. 2000

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A high sucrose diet reduces dentin apposition of growing rats. The mechanisms of reduction are unclear, but disturbances in calcium balance or in mineralization of predentin may explain them. In this experiment, 29 Sprague-Dawley rats, 21 days old, were weaned and randomized into calcium-deficient, high-sucrose or standard-diet groups for 3 weeks. They were given food and water ad libitum. During the experiment, animals were individually housed in metabolic cages where urine samples were collected. At ages of 21 and 40 days mineralizing dentin was marked using I.P. injections of oxytetracycline hydrochloride. At 42 days of age, the animals were anesthetized and their blood was collected by cardiac puncture. Right hemimandibles were sectioned sagittally and left hemimandibles were fixed, decalcified, and cut into histological sections. Dentin appositions were measured planimetrically, predentin width, from histological sections. Ca, K, and Na levels of serum and urine were measured flame photimetrically and P levels were measured by the UV method. Statistical analyses were done using one-way analysis of variation (ANOVA) Tuckey's HSD t test. In the calcium-deficient group, hypocalcemia, reduced dentin apposition, and increased predentin width were noticed when compared with the control group (P<0.05). Also, the increase in predentin width, caused by calcium deficiency, was significant compared with sucrose-fed animals (P<0.05). Sucrose diet reduced dentinogenesis, increased Ca excretion to urine, but also reduced urinary levels of P, K, and Na, and the differences were significant for the controls (P<0.05). In conclusion, despite the same kind of reduced dentinogenesis in calcium-deficient and high-sucrose groups, calcium imbalance or reduced mineralization of predentin does not explain reduced dentinogenesis in sucrose-fed animals.

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Section: Discussionmentioning

confidence: 96%

The Reducing Effects of a Calcium-Deficient Diet and High Sucrose Diet on Dentin Apposition of Rat Molars

et al. 2000

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“…After this period, eight animals were sacrificed and incisor samples were taken from the hemimandible of three of them (group E l ) chosen at random. (The other hemimandibles and the mandibles of the other five animals were used for the micro-hardness test; see Lozupone and Favia, 1994. ) Other incisor samples (group E2) were taken from the hemimandible of three of eight animals fed for an additional 10 days with a normal calcium diet (0.6% calcium, Altromin DP1000).…”

Section: Methodsmentioning

confidence: 99%

“…thickness (Lozupone and Favia, 1994). Thus the enamel matrix seems to retain its property to calcify a long time after its deposition.…”

mentioning

confidence: 99%

Morphological studies of hypomineralized enamel of rat pups on calcium‐deficient diet, and of its changes after return to normal diet

Bonucci¹,

Lozupone²,

Silvestrini³

et al. 1994

Anat. Rec.

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The results lead to the conclusions that: (1) E1 enamel is hypomineralized, and its degree of calcification is restored by return to a normal calcium diet; (2) intra- and interprismatic calcification occurs in a different way; (3) crystallite thickness is initially greater in dentinal than in the superficial zone and is reversed as crystallite growth is completed; and (4) loss of enamel proteins is necessary for completion of crystallite growth and not for crystallite formation.

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“…Vitamin A, zinc (Zn), and calcium (Ca) deficiencies have been studied, employing the rat as the experimental model [10][11][12][13][14][15]. Other minerals present in the diet such as boron (B) have received less attention [16].…”

Section: Introductionmentioning

confidence: 99%

Histomorphometric and Microchemical Characterization of Maturing Dental Enamel in Rats Fed a Boron-Deficient Diet

Durand¹,

Mesones²,

Nielsen³

et al. 2009

Biol Trace Elem Res

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Few reports are available in the literature on enamel formation under nutritional deficiencies. Thus, we performed a study to determine the effects of boron (B) deficiency on the maturing dental enamel, employing the rat continuously erupting incisor as the experimental model. Male Wistar rats, 21 days old, were used throughout. They were divided into two groups, each containing ten animals: +B (adequate; 3-mg B/kg diet) and -B (boron deficient; 0.07-mg B/kg diet). The animals were maintained on their respective diets for 14 days and then euthanized. The mandibles were resected, fixed, and processed for embedding in paraffin and/or methyl methacrylate. Oriented histological sections of the continuously erupting incisor were obtained at the level of the mesial root of the first molar, allowing access to the maturation zone of the developing enamel. Dietary treatment did not affect food intake and body weight. Histomorphometric evaluation using undecalcified sections showed a reduction in enamel thickness (hypoplasia), whereas microchemical characterization by energy-dispersive X-ray spectrometry did not reveal alterations in enamel mineralization.

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Morphometric analysis of the deposition and mineralization of enamel and dentine from rat incisor during the recovery phase following a low-calcium regimen

Cited by 26 publications

References 22 publications

The Reducing Effects of a Calcium-Deficient Diet and High Sucrose Diet on Dentin Apposition of Rat Molars

The Reducing Effects of a Calcium-Deficient Diet and High Sucrose Diet on Dentin Apposition of Rat Molars

Morphological studies of hypomineralized enamel of rat pups on calcium‐deficient diet, and of its changes after return to normal diet

Histomorphometric and Microchemical Characterization of Maturing Dental Enamel in Rats Fed a Boron-Deficient Diet

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