Morphometric and genetic variability among Mediterranean cereal cyst nematode ( Heterodera latipons ) populations in Turkey

Abstract

The Mediterranean cereal cyst nematode, Heterodera latipons, is an important plant parasitic nematode that negatively impacts cereal production worldwide, particularly in the Mediterranean Basin. Successful control of cereal cyst nematodes requires knowledge of the nematode species/pathotypes and their biology. However, little information is currently available concerning the phenotypic and genotypic variability of H. latipons populations in Turkey. This study analyzed the morphological/morphometric and genetic characteristics of Turkish H. latipons populations to define intraspecific distinctions between them. Morphological and morphometric studies focused on the pattern of the perineal region of cysts and second stage juveniles (J2), while the molecular analyses used sequenced ITS-rDNA regions targeting the internal transcribed spacer (ITS) region of rDNA. The data from these investigations demonstrate that nematode populations from Adana and Hatay provinces differ from the populations collected in Kilis, Gaziantep, and Mardin provinces. These phenotypic and genotypic differences between H. latipons populations may indicate that they are heterogenic, with at least two pathotypes. To date, this study is the most comprehensive analysis identifying H. latipons populations in major wheatproducing areas of Turkey.

Identification and genetic diversity of the Mediterranean cereal cyst nematode, Heterodera latipons Franklin, 1969 (Tylenchida: Heteroderidae) in cereal production areas of Northern Cyprus

“…Pratylenchus thornei, root lesion nematode, was determined to often co-occurred with H. latipons in the same area, which can also cause substantial yield losses of barley and wheat in Cyprus (Philis, 1988a(Philis, , 1995. This study demonstrated that surveyed cereal-growing areas were predominantly infested with the Mediterranean cereal cyst nematode, which complies with reports that found a relatively high density of the Mediterranean cereal cyst nematode in the samples obtained from cereal fields by Imren et al (2018) and Mokrini et al (2017) in Turkey and Morocco, respectively.…”

Journal of Plant Protection Research

“…The results of the present study did not find any polymorphism in H. filipjevi populations. In another study Imren et al (2012) reported that H. filipjevi populations from Iran and Turkey could cluster with a bootstrap value of 99%. Subbotin et al (2003) reported that the ITS sequence alignment of H. filipjevi isolates from Iran and Russia (Acc.…”

Effects of temperature and duration of storage on the hatching behaviour ofHeteroderalatipons (Nematoda: Heteroderidae)

“…The populations of H. latipons were sampled in 2016 growing season in wheat fields, which were identified to be historically infested with a high density (> 0.4 cyst g -1 of soil) of H. latipons (Table 1) (Imren et al, 2012(Imren et al, , 2018, located in four different provinces representing slightly diverse climatic zones: Adana, Hatay in Eastern-Mediterranean region, and Gaziantep and Kilis in South-Eastern region. At least 10 subsamples were randomly collected at depths of 0-20 cm to obtain a soil sample consisting of a minimum of 10 kg for each population.…”