Morphometric and molecular characterization of Parapharyngodon echinatus (Nematoda, Pharyngodonidae) from the Senegal gecko (Tarentola parvicarinata)

Abstract

A West African species of gecko, Tarentola parvicarinata Joger (Gekkonidae), collected in the Niokolo Koba National Park in East Senegal is a new host species for the nematode Parapharyngodon echinatus (Rudolphi, 1819). Fifty one specimens of P. echinatus were studied under a light microscope and thirteen specimens were studied for the first time by a scanning electron microscope. The main differences between P. echinatus and other African species, especially Parapharyngodon micipsae (Seurat, 1917), were in the shape of annules on body cuticle, broad lateral alae and their terminations, the shape of the distal extremity of the spicule and number of outgrowths at the anterior cloacal lip. For the purposes of DNA characterization, partial sequence of the small subunit ribosomal RNA gene (SSU rRNA) was obtained from two specimens of P. echinatus. This is the first published sequence of a species from the family Pharyngodonidae.

Molecular and ultrastructure characterization of two nematodes(Thelandros scleratus and Physalopteroides dactyluris)based on ribosomal and mitochondrial DNA sequences

“…This supports the previous fi ndings which already pointed on the closeness of these two genera (Chaudhary et al, 2014). In fact, the two genera are morphologically very similar (Mašová et al, 2008). Sequences of T. scleratus and Parapharyngodon spp.…”

The importance of integrative approaches in nematode taxonomy: the validity of Parapharyngodon and Thelandros as distinct genera

“…(2012), and observed under a light microscope (Olympus CX41, Olympus Australia Pty Ltd). Species identification was based on previously published traits (Roca, 1985; Moravec et al ., 1987; Astasio-Arbiza et al ., 1988, 1989; Solera-Puertas et al ., 1988; Mašová et al ., 2008, 2009; see table 2). For all individuals, the following body measurements were taken: body length (BL) and width at mid-body region (BW), tail length (TL), distance from nerve ring to mouth (NR), oesophageal bulb length (OBL) and width (OBW), and oesophagus length (OL) and width (OW).…”

Integrative approach on Pharyngodonidae (Nematoda: Oxyuroidea) parasitic in reptiles: Relationship among its genera, importance of their diagnostic features, and new data on Parapharyngodon bainae

“…Even though some studies deal with numerous sequences of the same gene from a single species [ 9 – 11 , 21 , 22 ], few species belonging to few genera of Pharyngodonidae have been genetically characterized (i.e. 5 genera out of 24) [ 9 , 10 , 20 – 23 ]. Furthermore, some available sequences (mainly those that have been not published in previous papers) are seemingly incorrect, e.g., the 28S sequences of P .…”