Morphometric and Molecular Study of Fasciola Isolates from Ruminants in Iran

Akhlaghi, Elham; Mohammadi, Mohammad Ali; Ziaali, Naser; Baneshi, Mohammad Reza; Nasibi, Saeid; Kamyabi, Hossein; Rostami, Sima; Harandi, Majid Fasihi

doi:10.5152/tpd.2017.5214

Cited by 10 publications

(5 citation statements)

References 13 publications

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“…Isolates for F. hepatica, 8 out of 10 were found in sheep. This was in agreement with studies from other regions of the world that shows F. hepatica are more prevalent in sheep while F. gigantica are more prevalent in cattle (Akhlaghi et al 2017). A study in Tanzania showed that 41 Fasciola isolates were F. hepatica (Farjallah et al 2009), in Iran showed that 96.8% of 31 sheep sampled were infected with F. hepatica (Rokni et al 2010).…”

Section: Discussionsupporting

confidence: 88%

Molecular characterization of Fasciola isolates collected from sheep, goats and cattle in Kisumu, Baringo and Narok Counties, Kenya

Kipyegen

Muleke

Otachi

2021

Preprint

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Fasciolosis is a neglected trematode infection of public health and veterinary importance caused by Fasciola gigantica and Fasciola hepatica. Molecular analysis using the internal transcribed spacers’ ITS-1 and ITS-2 of nuclear ribosomal DNA is useful in distinguishing Fasciola species. This study aimed to characterize liver flukes from sheep, goats and cattle using these genetic markers. Fifty nine adult Fasciola specimens were collected from livers of naturally infected sheep, goats and cattle at selected abattoirs in Kisumu, Baringo and Narok Counties. Sequence comparison of ITS-1 and ITS-2 sequences of Fasciola isolates from this study and sequences in Genbank was carried out. A maximum likelihood tree was constructed for phylogenetic analysis. Analysis of ITS-1 and ITS-2 rDNA sequences revealed that F. hepatica and F. gigantica caused infection in both cattle and sheep and in goats only F. gigantica caused infection. The sequenced PCR amplicons showed a close relationship between Fasciola species in this study with Fasciola isolates from other regions in the world. Phylogenetic analysis showed that sequences of F. hepatica are similar to the sequence from Spain, China and Tunisia obtained from GenBank. The sequences of F. gigantica in this study have similarity to the sequence from Iran and Burkina Faso. Data from this study provides information that serves as basis for further studies on the distribution of F. gigantica and F. hepatica in other localities in Kenya, and is also important in designing epidemiological and control programmes for zoonotic fascioliasis.

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Section: Discussionsupporting

confidence: 88%

Molecular characterization of Fasciola isolates collected from sheep, goats and cattle in Kisumu, Baringo and Narok Counties, Kenya

Kipyegen

Muleke

Otachi

2021

Preprint

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“…Morphometric features of two species were studied to identify presence of hybrid form where overlapping occurs. Parameters like specimen length, width, diameter of two suckers were considered practical criteria for differentiation between species of liver flukes (Akhlaghi et al 2017). Results of present study indicated that measurements differed considerably between two species at three different topographic locations.…”

Section: Discussionmentioning

confidence: 69%

“…Molecular techniques have greater sensitivity but not cost effective for routine use (El-Rahimy et al 2012). Characterization of fasciola species is useful for epidemiology, prevention and control of disease in endemic areas where hybridization phenomenon occurs (Akhlaghi et al 2017). Such hybrids/ intermediate forms were reported from Korea (Agatsuma et al 2000), Iran (Amor et al 2011), Veitnam (Le et al 2008), Egypt (Amer et al 2011) and China (Peng et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Identification of Fasciolids at Different Altitudes of Azad Jammu and Kashmir

2020

THE JAPS

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Fasciolosis is an important zoonotic disease affecting domestic animals in most parts of the world. Two important species of flukes Fasciola (F.) gigantic and Fasciola hepatica with an intermediate form was reported by many researchers from different countries of the world. For identification of species of fasciolids 167 flukes were collected from domestic animals at abattoirs from three topographic locations from three districts of Azad Jammu and Kashmir during 2012. Readings were taken using glass rulers and ocular micrometer. Characterization of F. hepatica revealed average length (mm) 20.66±2.27 to 23.20±5.25 and width 9.90±0.50 to 10.16±1.00 and that of F. gigantic 41.25±1.55 to 45.33±0.83, 9.44±0.20 to 9.71±0.37 respectively. It was noted that F. hepatica predominantly affecting animals at high altitudes with cooler climate and F. gigantica at low altitudes with warm conditions. No "intermediate form" of fasciola was found at any location or host animal. All flukes after morphometery were identified through PCR using species specific primers. Further investigations are required to investigate intermediate form from different geographic locations of Pakistan for control measures.

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“…The life cycle of Fasciola spp. involves a very wide range of domestic and wild mammals, including sheep, cattle, horse, camel, coypu, rabbit as well as humans as definitive hosts (Akhlaghi et al., 2018 ; Beesley et al., 2018 ; Mas‐Coma et al., 2020 ; Sabourin et al., 2018 ). Galba truncatula is the main intermediate host in the old world.…”

Section: Discussionmentioning

confidence: 99%

Morphological and molecular characterization of Fasciola isolates from livestock in Golestan province, northern Iran

Sharbatkhori

Nasibi

Mohammadi

et al. 2023

Veterinary Medicine & Sci

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Background: Fascioliasis, caused by the liver flukes Fasciola hepatica and Fasciola gigantica, is a global zoonotic helminthic disease. The livestock and human are the final hosts of the parasites. Northern Iran is an important endemic region for fascioliasis. Few studies have been conducted on the characterization of Fasciola isolates from eastern regions of the Caspian littoral of the country. Objective:The aim of the present study was to identify F. hepatica, F. gigantica and intermediate/hybrid forms of Fasciola isolates from livestock in Golestan province, northern Iran, using morphometric and molecular tools.Methods: Livestock livers naturally infected with Fasciola spp. were collected from Golestan slaughterhouse during 2019-2020. The worms were morphometrically studied using a calibrated stereomicroscope. Genomic DNA was extracted from all samples, and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was performed on internal transcribed spacer (ITS1) region using Rsa1 restriction enzyme. All the isolates were then analysed by multiplex PCR on Pepck region.Results: A total of 110 Fasciola isolates were collected from the infected livers, including 94 sheep, 12 cattle and 4 goats. Morphometric analysis of 61 adult Fasciola isolates indicated that, 44 and 17 isolates belonged to F. hepatica and F. gigantica, respectively.Eighty-one and 29 isolates belonged to F. hepatica and F. gigantica using ITS1-RFLP, respectively. However, Pepck Multiplex PCR indicated 72 F. hepatica, 26 F. gigantica and 12 intermediate/hybrid forms. All 12 hybrid isolates were found in sheep host. Two isolates were identified as F. gigantica using morphometry and F. hepatica using both molecular methods. Conclusion:The present study confirmed the existence of both F. hepatica and F. gigantica species and reported the first molecular evidence of hybrid Fasciola isolates in ruminants of Golestan province.

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Morphometric and Molecular Study of Fasciola Isolates from Ruminants in Iran

Cited by 10 publications

References 13 publications

Molecular characterization of Fasciola isolates collected from sheep, goats and cattle in Kisumu, Baringo and Narok Counties, Kenya

Molecular characterization of Fasciola isolates collected from sheep, goats and cattle in Kisumu, Baringo and Narok Counties, Kenya

Identification of Fasciolids at Different Altitudes of Azad Jammu and Kashmir

Morphological and molecular characterization of Fasciola isolates from livestock in Golestan province, northern Iran

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