Morphometric and ultrastructural features of the mare oviduct epithelium during oestrus

Desantis, Salvatore; Zizza, Sara; Accogli, Gianluca; Acone, F; Rossi, Roberta; Resta, Leonardo

doi:10.1016/j.theriogenology.2010.10.007

Cited by 25 publications

(33 citation statements)

References 38 publications

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“…Total cell numbers remained constant and the percentage of ciliated cells (relative to secretory cells) did not change in response to LNG treatment. Oestradiol and P4 control the transformation between ciliated and secretory cells, as well as the induction of ciliogenesis and deciliation in multiple mammalian oviduct epithelial cells, including those from horses, dogs and humans . However, there are no marked changes in the relative number of ciliated and secretory cells in any oviductal segment during the oestrous cycle in rats because of its short duration .…”

Section: Discussionmentioning

confidence: 99%

Levonorgestrel decreases cilia beat frequency of human fallopian tubes and rat oviducts without changing morphological structure

Zhao

Zhu

Yan

et al. 2015

Clin Exp Pharma Physio

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SUMMARYLevonorgestrel, a derivative of progesterone, effectively protects women against unwanted pregnancy as an emergency contraceptive. Previous studies have not been successful in determining the mechanism by which levonorgestrel acts. In the present study we analysed cilia beat action and cilia morphology following levonorgestrel exposure in vitro and in vivo using both light and electron microscopy. There was a significant decrease in the ciliary beat frequency (CBF) of human fallopian tubes between mucosal explants bathed in 5 lmol/L levonorgestrel and those bathed in medium alone (P < 0.05). There was a tendency for CBF to decrease more in the ampulla than in isthmus, but there were no differences between the proliferative and secretory phases. In rat oviducts, levonorgestrel produced a similar reduction in CBF (~10%) compared with the saline control group (P < 0.05). Histological and ultrastructural analysis demonstrated no changes in the percentage of ciliated cells or in the classic '9 + 2' structure of cilia following levonorgestrel treatment in either system. Thus, levonorgestrel reduces CBF without damaging cilia morphology. Decreases in CBF may indicate a pathological role for levonorgestrel in the transportation of the ovum and zygote in the fallopian tube.

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Section: Discussionmentioning

confidence: 99%

Levonorgestrel decreases cilia beat frequency of human fallopian tubes and rat oviducts without changing morphological structure

Zhao

Zhu

Yan

et al. 2015

Clin Exp Pharma Physio

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“…We analyzed the localization of DMBT1 in different sections of porcine oviduct (ampulla and isthmus) during the different estrous cycle stages. In both regions, the immunostaining was present mainly in non-ciliated cells, which are secretory cells mainly involved in the synthesis and release of glycoproteins that dissolve in the oviductal fluid (Buhi 2002, Abe & Hoshi 2007, Desantis et al 2011, but also in other cell types. The secretion of DMBT1 seems to follow the stages of the estrus cycle, as the presence of the protein is observed from the end of follicular phase in both isthmus and ampulla.…”

Section: Discussionmentioning

confidence: 99%

Deleted in malignant brain tumor 1 is secreted in the oviduct and involved in the mechanism of fertilization in equine and porcine species

Ambruosi¹,

Accogli²,

Douet³

et al. 2013

Reproduction

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Oviductal environment affects preparation of gametes for fertilization, fertilization itself, and subsequent embryonic development. The aim of this study was to evaluate the effect of oviductal fluid and the possible involvement of deleted in malignant brain tumor 1 (DMBT1) on IVF in porcine and equine species that represent divergent IVF models. We first performed IVF after pre-incubation of oocytes with or without oviductal fluid supplemented or not with antibodies directed against DMBT1. We showed that oviductal fluid induces an increase in the monospermic fertilization rate and that this effect is canceled by the addition of antibodies, in both porcine and equine species. Moreover, pre-incubation of oocytes with recombinant DMBT1 induces an increase in the monospermic fertilization rate in the pig, confirming an involvement of DMBT1 in the fertilization process. The presence of DMBT1 in the oviduct at different stages of the estrus cycle was shown by western blot and confirmed by immunohistochemical analysis of ampulla and isthmus regions. The presence of DMBT1 in cumulus-oocyte complexes was shown by western blot analysis, and the localization of DMBT1 in the zona pellucida and cytoplasm of equine and porcine oocytes was observed using immunofluorescence analysis and confocal microscopy. Moreover, we showed an interaction between DMBT1 and porcine spermatozoa using surface plasmon resonance studies. Finally, a bioinformatic and phylogenetic analysis allowed us to identify the DMBT1 protein as well as a DMBT1-like protein in several mammals. Our results strongly suggest an important role of DMBT1 in the process of fertilization.

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“…This result demonstrates a segment-specific distribution of the glycan pattern along the isthmus epithelium. Not all the oviductal NCs are secretory cells because some of them contain microvilli, which are covered with well-developed glycocalyx [11,60]. This suggests that NCs [61,62], which can be glycosylated [63][64][65].…”

Section: Discussionmentioning

confidence: 99%

Glycan profile of oviductal isthmus epithelium in normal and superovulated ewes

et al. 2016

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Morphometric and ultrastructural features of the mare oviduct epithelium during oestrus

Cited by 25 publications

References 38 publications

Levonorgestrel decreases cilia beat frequency of human fallopian tubes and rat oviducts without changing morphological structure

Levonorgestrel decreases cilia beat frequency of human fallopian tubes and rat oviducts without changing morphological structure

Deleted in malignant brain tumor 1 is secreted in the oviduct and involved in the mechanism of fertilization in equine and porcine species

Glycan profile of oviductal isthmus epithelium in normal and superovulated ewes

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