Morphometric Study of the Jejunal Mucosa in Various Childhood Enteropathies with Special Reference to Intraepithelial Lymphocytes

Predictors and Significance of Incomplete Mucosal Recovery in Celiac Disease After 1 Year on a Gluten-Free Diet

“…Th e fi nal morphological analysis was conducted on the most severely damaged biopsy specimen ( 16 ). Normal Vh/CrD was considered to be over 2.0 ( 17 ). To ascertain a dose response refl ected in outcome measures, the Atrophy group were further categorized into those with subtotal or total villous atrophy (Vh/CrD below 0.9) and those with partial villous atrophy (Vh/CrD 0.9-1.9).…”

Degree of Damage to the Small Bowel and Serum Antibody Titers Correlate With Clinical Presentation of Patients With Celiac Disease

“…A ratio of 2.0 or more was considered normal. 9 Furthermore, the mucosal specimens were graded independently according to the Marsh-Oberhuber classification as follows: Marsh 0 denotes normal villi and crypt depth with no excess of intraepithelial lymphocytes (IEL), Marsh I-II denotes normal villi but intraepithelial lymphocytosis without (I) or with (II) hyperplastic crypts, and Marsh IIIa-c denotes varying degrees of villous atrophy with hyperplastic crypts. 10 Three biopsy specimens were freshly embedded in optimal cutting temperature compound, snap-frozen in liquid nitrogen, and stored at Ϫ70°C.…”

Endomysial antibody-negative coeliac disease: clinical characteristics and intestinal autoantibody deposits

“…In addition, to study the mucosal histology more objectively, the villous height:crypt depth ratio was determined from well-oriented biopsy samples from multiple sites. 21 A ratio ,2 was considered to be compatible with coeliac disease.…”