Mosquito vector competence for dengue is modulated by insect-specific viruses

Olmo, Roenick Proveti; Todjro, Yaovi M H; Aguiar, Eric Roberto Guimarães Rocha; Almeida, João Fernando Lourenço de; Ferreira, Flávia V; Armache, Juliana N.; Faria, Isaque J S de; Ferreira, Álvaro G. A.; Amadou, Siad C. G.; Silva, Ana Teresa S; Souza, Kátia Paulino Ribeiro de; Vilela, Ana Paula Pessoa; Babarit, Antinéa; Tan, Cheong Huat; Diallo, Mawlouth; Gaye, Alioune; Paupy, Christophe; Obame-Nkoghe, Judicaël; Visser, Tessa M.; Koenraadt, Constantianus J. M.; Wongsokarijo, Merril; Cruz, Ana Luiza C; Prieto, Mariliza T; Parra, Maisa Carla Pereira; Nogueira, Maurício Lacerda; Avelino‐Silva, Vivian Iida; Mota, Renato N; Borges, Magno Augusto Zazá; Drumond, Betânia Paiva; Kroon, Erna Geessien; Recker, Mario; Sedda, Luigi; Marois, Eric; Imler, Jean‐Luc; Marques, João Trindade

doi:10.1038/s41564-022-01289-4

Cited by 79 publications

(70 citation statements)

References 78 publications

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“…Similarly, future work could examine trends in how coinfection dynamics are studied and tested. Both insect-specific viruses and Wolbachia have been considered as potential biological countermeasures to arboviral transmission in mosquitoes [45][46][47][48] ; however, the network of pathogencoinfection-vector combinations has been characterized even less systematically, and these data remain largely unsynthesized. Addressing these types of questions in the future may point to new opportunities for both empirical research and modeling that harnesses these data to predict and prevent arboviral emergence.…”

Section: Discussionmentioning

confidence: 99%

Exploring the Mosquito–Arbovirus Network: A Survey of Vector Competence Experiments

Chen

Sweeny

et al. 2023

The American Journal of Tropical Medicine and Hygiene

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Arboviruses receive heightened research attention during major outbreaks or when they cause unusual or severe clinical disease, but they are otherwise undercharacterized. Global change is also accelerating the emergence and spread of arboviral diseases, leading to time-sensitive questions about potential interactions between viruses and novel vectors. Vector competence experiments help determine the susceptibility of certain arthropods to a given arbovirus, but these experiments are often conducted in real time during outbreaks, rather than with preparedness in mind. We conducted a systematic review of reported mosquito–arbovirus competence experiments, screening 570 abstracts to arrive at 265 studies testing in vivo arboviral competence. We found that more than 90% of potential mosquito–virus combinations are untested in experimental settings and that entire regions and their corresponding vectors and viruses are undersampled. These knowledge gaps stymie outbreak response and limit attempts to both build and validate predictive models of the vector–virus network.

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Section: Discussionmentioning

confidence: 99%

Exploring the Mosquito–Arbovirus Network: A Survey of Vector Competence Experiments

Chen

Sweeny

et al. 2023

The American Journal of Tropical Medicine and Hygiene

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“…Taken together, the early reports along with recent evidence suggest that mosquito vectors in Panama may be infected with MADV at a lower frequency when compared with VEEV ID subtype and even MADV in other endemic regions. The reasons for this variation in MADV and VEEV ID subtype frequency of isolation or detection from mosquitoes collected in Panama remain unclear and may include variation in vector competence or viral heterologous competition or even enhanced VEEV ID subtype transmission by insect specific virus as has been shown with dengue and Zika viruses (42).…”

Section: Discussionmentioning

confidence: 99%

Venezuelan equine encephalitis complex, Madariaga and Eastern equine encephalitis viruses genome detection in human and mosquito populations

Carrera

Araúz²,

Rojas

et al. 2022

Preprint

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Background. Venezuelan equine encephalitis (VEE) complex, eastern equine encephalitis virus (EEEV), and Maradiaga virus (MADV), are associated with severe neurological disease in human and equine hosts. We aimed to overcome the lack of available molecular diagnostics for these pathogens by designing and clinically evaluating real-time reverse transcription PCRs (rRT-PCRs) for 1) VEE complex and 2) MADV/EEEV. Methodology/Principal Findings. Primers and probes were designed from alignments of all publicly available complete genome sequences for VEE complex, EEEV and MADV. Linear range for the VEE complex and MADV/EEEV rRT-PCRs extended from 2.0 to 8.0 log10 copies/uL with exclusive detection of the respective viruses. Fifteen serum samples from febrile patients collected from 2015 and 2017 outbreaks in Panama were tested to evaluate the new assays. Ten samples (66.7%) samples were positive for VEEV, and in one sample, rRT-PCR detected both VEEV and MADV. Early acute samples (≤ 3 days since onset) with coincidental anti-VEEV IgM and IgG antibodies were also found positive for VEEV viral RNA. Of 150 mosquito pools, 3 tested positive for VEEV by rRT-PCR. Two of these also yielded VEEV isolates. Conclusions. The VEE complex and MADV/EEEV rRT-PCRs provide accurate detection while yielding significant benefits over available molecular methods. Data from the clinical evaluation provide further evidence for VEE complex and MADV co-infections and suggest that re-infection with VEE complex viruses is possible.

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“…In addition to their role in disease transmission, mosquitos also harbour a highly diverse virome, encompassing many "insect-specific" viruses that are not associated with the infection of vertebrates 2,3 . Although these insect-specific viruses do not directly impact public health, some are known to influence the transmission of arboviruses 4 .…”

Section: Introductionmentioning

confidence: 99%

Metagenomic analysis of individual mosquitos reveals the ecology of insect viruses

Pan,

Zhao,

Gou

et al. 2023

Preprint

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Mosquito transmitted viruses are responsible for an increasing burden of human disease. Despite this, little is known about the diversity and ecology of viruses within individual mosquito hosts. Using a meta-transcriptomic approach, we analysed the virome of 2,438 individual mosquitos (79 species), spanning ∼4000 km along latitudes and longitudes in China. From these data we identified 393 core viral species associated with mosquitos, including seven (putative) arbovirus species. We identified potential species and geographic hotspots of viral richness and arbovirus occurrence, and demonstrated that host phylogeny had a strong impact on the composition of individual mosquito viromes. Our data revealed a large number of viruses shared among mosquito species or genera, expanding our knowledge of host specificity of insect-associated viruses. We also detected multiple virus species that were widespread throughout the country, possibly facilitated by long-distance mosquito migrations. Together, our results greatly expand the known mosquito virome, linked the viral diversity at the scale of individual insects to that at a country-wide scale, and offered unique insights into the ecology of viruses of insect vectors.

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Mosquito vector competence for dengue is modulated by insect-specific viruses

Cited by 79 publications

References 78 publications

Exploring the Mosquito–Arbovirus Network: A Survey of Vector Competence Experiments

Exploring the Mosquito–Arbovirus Network: A Survey of Vector Competence Experiments

Venezuelan equine encephalitis complex, Madariaga and Eastern equine encephalitis viruses genome detection in human and mosquito populations

Metagenomic analysis of individual mosquitos reveals the ecology of insect viruses

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