Moss (<i>Physcomitrella patens</i>) Expressed Sequence Tags Include Several Sequences which are Novel for Plants*

Reski, Ralf; Reynolds, S.; Wehe, M.; Kleber-Janke, Tamara; Kruse, Sven

doi:10.1111/j.1438-8677.1998.tb00689.x

Cited by 38 publications

(26 citation statements)

References 32 publications

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“…RNA blot hybridization was carried out as described (39) with the following radioactively labeled cDNA probes: PpCOR47, PpCOR-TMC AP3 (39), the homolog to ribosomal protein L21 (32) amplified from cDNA with the primers 5′-GGTTGGTCATGGGTTGCG-3′ and 5′-GAGGTCAACTGTCTCGCC-3′, and a PCA1 cDNA fragment amplified using the primers 5′-TTGCGATTGGCTTCATAAGCTG-3′ and 5′-ACGGTAAAAGA-CAACGATCAAGTT-3′. Signal intensities were quantified by using the Quantity One software package (BioRad).…”

Section: Methodsmentioning

confidence: 99%

A P _IIB -type Ca ²⁺ -ATPase is essential for stress adaptation in Physcomitrella patens

Qudeimat

Faltusz

Wheeler

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

102

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Transient cytosolic Ca 2+ ([Ca 2+ ] cyt ) elevations are early events in plant signaling pathways including those related to abiotic stress. The restoration of [Ca 2+ ] cyt to prestimulus levels involves ATP-driven Ca 2+ pumps, but direct evidence for an essential role of a plant Ca 2+ -ATPase in abiotic stress adaptation is missing. Here, we report on a stress-responsive Ca 2+ -ATPase gene ( PCA1 ) from the moss Physcomitrella patens. Functional analysis of PCA1 in a Ca 2+ transport-deficient yeast mutant suggests that PCA1 encodes a P IIB -type Ca 2+ -ATPase harboring an N-terminal autoinhibitory domain. In vivo localizations identified membranes of small vacuoles as the integration site for a PCA1:GFP fusion protein. PCA1 mRNA levels are up-regulated by dehydration, NaCl, and abscisic acid, and PCA1 loss-of-function mutants (Δ PCA1 ) exhibit an enhanced susceptibility to salt stress. The Δ PCA1 lines show sustained elevated [Ca 2+ ] cyt in response to salt treatment in contrast to WT that shows transient Ca 2+ elevations, indicating a direct role for PCA1 in the restoration of prestimulus [Ca 2+ ] cyt . The altered Ca 2+ response of the Δ PCA1 mutant lines correlates with altered expression levels of stress-induced genes, suggesting disturbance of a stress-associated signaling pathway. We propose that PCA1 is an essential component for abiotic stress adaptation in Physcomitrella involved in the generation of a specific salt-induced Ca 2+ signature.

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Section: Methodsmentioning

confidence: 99%

A P _IIB -type Ca ²⁺ -ATPase is essential for stress adaptation in Physcomitrella patens

Qudeimat

Faltusz

Wheeler

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

102

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“…A high degree of conservation between moss and seed plant sequences has been observed. The intron-exon structure and the codon usage in mosses (P. patens and Tortula ruralis) are very similar to the dicotyledenous plants (Reski et al, 1998, Wood et al, 1999.…”

Section: Bryophytes As An Ancient Lineage May Have Retained Many Novementioning

confidence: 99%

Hormonal regulation in green plant lineage families

Johri

2008

Physiol Mol Biol Plants

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The patterns of phytohormones distribution, their native function and possible origin of hormonal regulation across the green plant lineages (chlorophytes, charophytes, bryophytes and tracheophytes) are discussed. The five classical phytohormonesauxins, cytokinins, gibberellins (GA), abscisic acid (ABA) and ethylene occur ubiquitously in green plants. They are produced as secondary metabolites by microorganisms. Some of the bacterial species use phytohormones to interact with the plant as a part of their colonization strategy. Phytohormone biosynthetic pathways in plants seem to be of microbial origin and furthermore, the origin of high affinity perception mechanism could have preceded the recruitment of a metabolite as a hormone. The bryophytes represent the earliest land plants which respond to the phytohormones with the exception of gibberellins. The regulation by auxin and ABA may have evolved before the separation of green algal lineage. Auxin enhances rhizoid and caulonemal differentiation while cytokinins enhance shoot bud formation in mosses. Ethylene retards cell division but seems to promote cell elongation. The presence of responses specific to cytokinins and ethylene strongly suggest the origin of their regulation in bryophytes. The hormonal role of GAs could have evolved in some of the ferns where antheridiogens (compounds related to GAs) and GAs themselves regulate the formation of antheridia.During migration of life forms to land, the tolerance to desiccation may have evolved and is now observed in some of the microorganisms, animals and plants. Besides plants, sequences coding for late embryogenesis abundant-like proteins occur in the genomes of other anhydrobiotic species of microorganisms and nematodes. ABA acts as a stress signal and increases rapidly upon desiccation or in response to some of the abiotic stresses in green plants. As the salt stress also increases ABA release in the culture medium of cyanobacterium Trichormus variabilis, the recruitment of ABA in the regulation of stress responses could have been derived from prokaryotes and present at the level of common ancestor of green plants. The overall hormonal action mechanisms in mosses are remarkably similar to that of the higher plants. As plants are thought to be monophyletic in origin, the existence of remarkably similar hormonal mechanisms in the mosses and higher plants, suggests that some of the basic elements of regulation cascade could have also evolved at the level of common ancestor of plants. The networking of various steps in a cascade or the crosstalk between different cascades is variable and reflects the dynamic interaction between a species and its specific environment.

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“…Preliminary analysis of genes and ESTs strongly indicate that these sequences are highly similar to those of the corresponding higher plant genes. These similarities extend to codon usage (68,96), intron-exon structure (18,61,67), and multigene family complexity (18,61,95). It is clear that the number of Physcomitrella genes and EST sequences accessible in the database will increase rapidly, and each entry directly enables the generation of the corresponding moss mutant by targeted transgenesis.…”

Section: The Physcomitrella Patens Model Systemmentioning

confidence: 99%

A NEWMOSSGENETICS: Targeted Mutagenesis inPhyscomitrella patens

Schaefer

2002

Annu. Rev. Plant Biol.

162

110

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s Abstract The potential of moss as a model system to study plant biology is associated with their relatively simple developmental pattern that nevertheless resembles the basic organization of the body plan of land plants, the direct access to cell-lineage analysis, their similar responses to plant growth factors and environmental stimuli as those observed in other land plants, and the dominance of the gametophyte in the life cycle that facilitates genetic approaches. Transformation studies in the moss Physcomitrella patens have revealed a totally unique feature for plants, i.e., that foreign DNA sequences integrate in the genome preferentially at targeted locations by homologous recombination, enabling for the first time in plants the application of the powerful molecular genetic approaches used routinely in bacteria, yeast, and since 1989, the mouse embryonic stem cells. This article reviews our current knowledge of Physcomitrella patens transformation and its unique suitability for functional genomic studies.

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Moss (Physcomitrella patens) Expressed Sequence Tags Include Several Sequences which are Novel for Plants*

Cited by 38 publications

References 32 publications

A P _IIB -type Ca ²⁺ -ATPase is essential for stress adaptation in Physcomitrella patens

A P _IIB -type Ca ²⁺ -ATPase is essential for stress adaptation in Physcomitrella patens

Hormonal regulation in green plant lineage families

A NEWMOSSGENETICS: Targeted Mutagenesis inPhyscomitrella patens

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Moss (Physcomitrella patens) Expressed Sequence Tags Include Several Sequences which are Novel for Plants*

Cited by 38 publications

References 32 publications

A P IIB -type Ca 2+ -ATPase is essential for stress adaptation in Physcomitrella patens

A P IIB -type Ca 2+ -ATPase is essential for stress adaptation in Physcomitrella patens

Hormonal regulation in green plant lineage families

A NEWMOSSGENETICS: Targeted Mutagenesis inPhyscomitrella patens

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A P _IIB -type Ca ²⁺ -ATPase is essential for stress adaptation in Physcomitrella patens

A P _IIB -type Ca ²⁺ -ATPase is essential for stress adaptation in Physcomitrella patens