Motility is an important determinant for pathogenesis of Erwinia carotovora subsp. carotovora

Hossain, Md. Mijan; Shibata, Satoshi; Aizawa, Shin‐Ichi; Tsuyumu, Shinji

doi:10.1016/j.pmpp.2005.06.001

Cited by 58 publications

(46 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It has been recently shown that the FliC homolog of Erwinia carotovora subsp. carotovora (64% identity, 79% similarity to E. chrysanthemi FliC) is essential for motility (30). An increased level of yet another transcript, namely that of gltA, encoding citrate synthase correlates well with the fact that citrate is the precursor for achromobactin.…”

Section: Discussionmentioning

confidence: 99%

TheErwinia chrysanthemi3937 PhoQ Sensor Kinase Regulates Several Virulence Determinants

Balakrishnan

Babujee

Liu³

et al. 2006

J Bacteriol

Self Cite

View full text Add to dashboard Cite

The PhoPQ two-component system regulates virulence factors in Erwinia chrysanthemi, a pectinolytic enterobacterium that causes soft rot in several plant species. We characterized the effect of a mutation in phoQ, the gene encoding the sensor kinase PhoQ of the PhoPQ two-component regulatory system, on the global transcriptional profile of E. chrysanthemi using cDNA microarrays and further confirmed our results by quantitative reverse transcription-PCR analysis. Our results indicate that a mutation in phoQ affects transcription of at least 40 genes, even in the absence of inducing conditions. Enhanced expression of several genes involved in iron metabolism was observed in the mutant, including that of the acs operon that is involved in achromobactin biosynthesis and transport. This siderophore is required for full virulence of E. chrysanthemi, and its expression is governed by the global repressor protein Fur. Changes in gene expression were also observed for membrane transporters, stress-related genes, toxins, and transcriptional regulators. Our results indicate that the PhoPQ system governs the expression of several additional virulence factors and may also be involved in interactions with other regulatory systems.

show abstract

Section: Discussionmentioning

confidence: 99%

TheErwinia chrysanthemi3937 PhoQ Sensor Kinase Regulates Several Virulence Determinants

Balakrishnan

Babujee

Liu³

et al. 2006

J Bacteriol

Self Cite

View full text Add to dashboard Cite

show abstract

“…Unlike the model bacterial plant pathogens, P. syringae, SRE are motile during infection (99), but whether they secrete proteases or plant cell wall--degrading enzymes while motile remains unknown. Motility itself, but not the presence of flagella, is required for the virulence of some Dickeya and Pectobacterium, suggesting that the agitation provided by the motile cells may assist in disease development (60). The flagellar secretion system is also tied to microbe-microbe interactions; it is the secretion system used to deliver colicin, a microbial toxin that kills closely related bacterial species (17).…”

Section: Type III Secretion System: Disease Through Elicitation Of Plmentioning

confidence: 99%

The Role of Secretion Systems and Small Molecules in Soft-Rot Enterobacteriaceae Pathogenicity

Charkowski¹,

Blanco

Condemine

et al. 2012

Annu. Rev. Phytopathol.

Self Cite

220

251

View full text Add to dashboard Cite

Soft-rot Enterobacteriaceae (SRE), which belong to the genera Pectobacterium and Dickeya, consist mainly of broad host-range pathogens that cause wilt, rot, and blackleg diseases on a wide range of plants. They are found in plants, insects, soil, and water in agricultural regions worldwide. SRE encode all six known protein secretion systems present in gram-negative bacteria, and these systems are involved in attacking host plants and competing bacteria. They also produce and detect multiple types of small molecules to coordinate pathogenesis, modify the plant environment, attack competing microbes, and perhaps to attract insect vectors. This review integrates new information about the role protein secretion and detection and production of ions and small molecules play in soft-rot pathogenicity.

show abstract

“…In addition, motility and some effectors secreted through the type III secretion system also augment virulence of E. carotovora subsp. carotovora (12,37,65).The regulation of the extracellular enzymes and proteins including Harpin, the elicitor of the hypersensitive response, in E. carotovora subsp. carotovora has been extensively studied, and many regulatory genes and factors have been identified.…”

mentioning

confidence: 99%

“…In addition, motility and some effectors secreted through the type III secretion system also augment virulence of E. carotovora subsp. carotovora (12,37,65).…”

mentioning

confidence: 99%

“…Flagellar motility is an accessory virulence determinant in animal pathogens, as well as in several plant pathogens, including E. carotovora (31,37,40,44,52,53,59). The FlhDC complex, comprising the products of flhD and flhC, is the master regulatory operon controlling the expression of flagellar genes in Escherichia coli and Salmonella enterica serovar Typhimurium (5,7,18,53).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Regulatory Network Controlling Extracellular Proteins inErwinia carotovorasubsp.carotovora: FlhDC, the Master Regulator of Flagellar Genes, ActivatesrsmBRegulatory RNA Production by AffectinggacAandhexA(lrhA) Expression

et al. 2008

View full text Add to dashboard Cite

Erwinia carotovora subsp. carotovora produces an array of extracellular proteins (i.e., exoproteins), including plant cell wall-degrading enzymes and Harpin, an effector responsible for eliciting hypersensitive reaction. Exoprotein genes are coregulated by the quorum-sensing signal, N-acyl homoserine lactone, plant signals, an assortment of transcriptional factors/regulators (GacS/A, ExpR1, ExpR2, KdgR, RpoS, HexA, and RsmC) and posttranscriptional regulators (RsmA, rsmB RNA). rsmB RNA production is positively regulated by GacS/A, a two-component system, and negatively regulated by HexA (PecT in Erwinia chrysanthemi; LrhA [LysR homolog A] in Escherichia coli) and RsmC, a putative transcriptional adaptor. While free RsmA, an RNA-binding protein, promotes decay of mRNAs of exoprotein genes, binding of RsmA with rsmB RNA neutralizes the RsmA effect. In the course of studies of GacA regulation, we discovered that a locus bearing strong homology to the flhDC operon of E. coli also controls extracellular enzyme production. A transposon insertion FlhDC ؊ mutant produces very low levels of pectate lyase, polygalacturonase, cellulase, protease, and E. carotovora subsp. carotovora Harpin (Harpin Ecc ) and is severely attenuated in its plant virulence. The production of these exoproteins is restored in the mutant carrying an FlhDC ؉ plasmid. Sequence analysis and transcript assays disclosed that the flhD operon of E. carotovora subsp. carotovora, like those of other enterobacteria, consists of flhD and flhC. Complementation analysis revealed that the regulatory effect requires functions of both flhD and flhC products. The data presented here show that FlhDC positively regulates gacA, rsmC, and fliA and negatively regulates hexA (lrhA). Evidence shows that FlhDC controls extracellular protein production through cumulative effects on hexA and gacA. Reduced levels of GacA and elevated levels of HexA in the FlhDC ؊ mutant are responsible for the inhibition of rsmB RNA production, a condition conducive to the accumulation of free RsmA. Indeed, studies with an RsmA ؊ FlhDC ؊ double mutant and multiple copies of rsmB ؉ DNA establish that the negative effect of FlhDC deficiency is exerted via RsmA. The FlhDC-mediated regulation of fliA has no bearing on exoprotein production in E. carotovora subsp. carotovora. Our observations for the first time establish a regulatory connection between FlhDC, HexA, GacA, and rsmB RNA in the context of the exoprotein production and virulence of E. carotovora subsp. carotovora.Erwinia carotovora subsp. carotovora (Ecc), a member of the Enterobacteriaceae family, causes soft-rotting disease on a wide variety of plants worldwide. A consortium of plant cell walldegrading extracellular enzymes comprising pectate lyase (Pel), polygalacturonase (Peh), protease (Prt), and cellulase (Cel) contribute to its plant virulence (4,15,19,61,71). Among them, extracellular pectinases, including Pel and Peh, play a crucial role in tissue maceration and cell death. In addition, motility and some effectors secreted th...

show abstract

Motility is an important determinant for pathogenesis of Erwinia carotovora subsp. carotovora

Cited by 58 publications

References 55 publications

TheErwinia chrysanthemi3937 PhoQ Sensor Kinase Regulates Several Virulence Determinants

TheErwinia chrysanthemi3937 PhoQ Sensor Kinase Regulates Several Virulence Determinants

The Role of Secretion Systems and Small Molecules in Soft-Rot Enterobacteriaceae Pathogenicity

Regulatory Network Controlling Extracellular Proteins inErwinia carotovorasubsp.carotovora: FlhDC, the Master Regulator of Flagellar Genes, ActivatesrsmBRegulatory RNA Production by AffectinggacAandhexA(lrhA) Expression

Contact Info

Product

Resources

About