Mould extracts increase the allergic response to ovalbumin in mice

Instanes, Christine; Ormstad, Heidi; Rydjord, B.; Wiker, Harald G.; Hetland, Geir

doi:10.1111/j.1365-2222.2004.02076.x

Cited by 33 publications

(38 citation statements)

References 35 publications

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“…A phenomenon that is common in allergy studies has been the observation that greater adjuvant responses occur at lower doses compared to higher doses. Instanes et al [16] indicated that the adjuvant effect of mold extracts was most pronounced at the lowest dose (12.5 µg) examined, which was comparable to the peak adjuvant dose of 10 µg of zymosan in the current study.…”

Section: Resultssupporting

confidence: 75%

“…A recent study by Huang et al [28] indicated that the adjuvant activity of purified 1→3-β-glucan led to a Th1/Th17 response, and not the Th2 response as reported in this paper and by others [14][15][16] for zymosan, further differentiating the activities of 1→3-β-glucan from zymosan. It was observed that OVA-loaded purified β-glucan particles induced strong humoral and Th1-and Th17-dependent CD4+ T-cell responses in mice.…”

Section: Resultssupporting

confidence: 60%

“…Ormstad et al [15] demonstrated that a crude β-glucan suspension extracted from barley enhanced a Th2-dependent antibody response to OVA, as indicated by increased levels of IgE and IgG1 compared to OVA. In a related investigation, Instanes et al [16] observed that soluble extracts of different molds had adjuvant effects on the allergic response to OVA in mice. Wan et al [14] utilized an airway OVA mouse allergy model by which animals were sensitized by an aerosolized crude β-glucan extract and OVA.…”

Section: Resultsmentioning

confidence: 97%

“…In a comparison of the pulmonary inflammatory potential of different components of yeast and fungal cell walls, 1→3-β-glucan was the most potent inflammatory agent tested, and zymosan, the crude B-glucan preparation, had a greater inflammatory activity than a partially purified particulate 1→3-β-glucan [13]. In addition, specific β-glucans [14,15] and soluble mold extracts [16] have been observed to have adjuvant effects in an allergic response to ovalbumin (OVA) in mice.…”

Section: Introductionmentioning

confidence: 99%

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Adjuvant effect of zymosan after pulmonary treatment in a mouse ovalbumin allergy model

Young

Wolfarth

Roberts

et al. 2013

Experimental Lung Research

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An association has been observed between indoor mold contamination and lung allergy and asthma. This relationship is not fully understood. 1→3-β-Glucan is the major cell wall component of fungi and a good marker of fungi exposure. The objective was to evaluate the adjuvant effect of zymosan, a crude yeast cell wall preparation of 1→3-β-glucan, during ovalbumin (OVA) sensitization in an allergy model. BALB/c mice were sensitized by pharyngeal aspiration with saline, 50 µg of OVA, or OVA with 1, 10, 50, or 75 µg of zymosan on days 0, 7, and 14. One week after sensitization, each sensitized animal group was challenged with an aspiration dose of 50 µg of OVA once a week for 2 weeks. At 1 day after the last aspiration, bronchoalveolar lavage fluid and blood was collected, and markers of lung allergy and inflammation were assessed. An adjuvant effect of zymosan on OVA allergy during sensitization was observed as indicated by significant elevations in lung eosinophils, serum OVA-specific IgE, and lung IL-5 in the groups sensitized with zymosan and OVA. Pulmonary treatment with zymosan also amplified lung inflammation. Elevations were observed in lung neutrophils, TNF-α, and parameters of lung injury in the groups primed with both zymosan and OVA. In nearly all parameters, a non-linear dose-response relationship was observed in the groups primed with OVA and zymosan. The optimum adjuvant dose of zymosan was 10 µg. This study demonstrated an adjuvant effect of zymosan when exposures occurred during the sensitization phase in an OVA-induced allergy model in BALB/c mice.

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Section: Resultssupporting

confidence: 75%

Section: Resultssupporting

confidence: 60%

Section: Resultsmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Adjuvant effect of zymosan after pulmonary treatment in a mouse ovalbumin allergy model

Young

Wolfarth

Roberts

et al. 2013

Experimental Lung Research

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“…However, the anti-allergic effects of Andosan, descibed below, are most probably not due to β-glucans because we have previously found that β-glucans from yeast rather increased the allergic response to ovalbumin in mice [51]. As suggested below, smaller molecules may be responsible for these effects.…”

Section: Mechanism Of Action-immunomodulationmentioning

confidence: 93%

Andosan?-An Anti-Allergic and Anti-Inflammatory Ingredient Prepared from Agaricus blazei Mushroom

Hetl

Therkelsen²,

Nentvich³

et al. 2015

J Clin Cell Immunol

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Andosan™ is an extract prepared from medicinal Basidiomycetes mushrooms, mainly Agaricus blazei Murill (AbM) (82%), but it also contains Hericeum erinaceus (He) (15%) and Grifola frondosa (Gf) (3%), all of which have immunomodulating properties. Whereas He and Gf have been used in traditional Chinese and Japanese medicine against cancer, AbM originates from Brazilian rain forest and was used locally and later abroad as health food for the prevention of a range of diseases, including chronic hepatitis, diabetes and cancer. The mushrooms' health effects are probably due to their content of β-glucans and other smaller immunomodulating polysaccharides and proteoglucans. These effects are mediated through their stimulation of innate immune cells, such as monocytes, NK cells and dendritic cells, and the amelioration of a skewded Th1/Th2 balance and of inflammation. Here, we give an overview of the anti-allergic effects of Andosan™ in a mouse model for allergy and anti-inflammatory effects both in vitro, in healthy individuals and in patients with inflammatory bowel diseases.

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Yeasts in Food and Beverages

Querol¹,

Fleet²

2006

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Mould extracts increase the allergic response to ovalbumin in mice

Abstract: Our results show that extracts from CH and PC, and the beta-1,3/1,6-glucan from baker's yeast have adjuvant effects on the allergic response in mice.

Cited by 33 publications

References 35 publications

Adjuvant effect of zymosan after pulmonary treatment in a mouse ovalbumin allergy model

Adjuvant effect of zymosan after pulmonary treatment in a mouse ovalbumin allergy model

Andosan?-An Anti-Allergic and Anti-Inflammatory Ingredient Prepared from Agaricus blazei Mushroom

Yeasts in Food and Beverages

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