Mouse antibody production test: can we do without it?

Mahabir, Esther; Jacobsen, K; Brielmeier, Markus; Peters, D.; Needham, J. R.; Schmidt, Jörg

doi:10.1016/j.jviromet.2004.05.006

Cited by 14 publications

(11 citation statements)

References 17 publications

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“…Prior to the test, the serum samples were inactivated by heating to 568C for 30 min and diluted 1:10 in PBS (Oxoid, Hants, U.K.) containing 0.05% Tween 20 (R&L Slaughter, Essex, U.K.). Sera were tested for specific antibodies to MHV by use of an enzyme-linked immunosorbent assay (ELISA) using nonviral control and viral antigens and negative and positive serum (Churchill; Applied Biotechnology Ltd. Cambridgeshire, U.K.) [39]. The control sera undergo quality-control tests (ISO accreditation).…”

Section: Serological Analysismentioning

confidence: 99%

Risk Assessment of Mouse Hepatitis Virus Infection via In Vitro Fertilization and Embryo Transfer by the Use of Zona-Intact and Laser-Microdissected Oocytes1

Peters

Marschall

Mahabir

et al. 2006

Biology of Reproduction

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The aim of this study was to estimate the risk of mouse hepatitis virus (MHV) transmission by the in vitro fertilization and embryo transfer (IVF-ET) procedure. In addition, resistance to infection of zona-intact and laser-microdissected oocytes was compared. For this purpose, infectious mouse hepatitis virus, a common viral pathogen in mouse facilities, was used. Oocytes having an intact or laser-microdissected zona pellucida were incubated for fertilization in media containing MHV-A59 and resulting embryos were transferred to the oviduct of specific pathogen-free (SPF) Swiss recipients. The oocytes were divided into three experimental groups: 1) zona-intact oocytes continuously exposed to MHV in fertilization (HTF), culture (KSOM), and embryo transfer (M2) media; 2) zona-intact oocytes exposed to MHV in HTF medium and transferred after a standard washing procedure with virus-free KSOM and M2; and 3) laser-microdissected oocytes exposed to MHV in HTF medium and transferred after a standard washing procedure with virus-free KSOM and M2. Respective serum samples of embryo recipients and their offspring were tested for MHV antibodies using ELISA. In experiment 1, 10 out of 14 embryo recipients seroconverted to MHV and only their offspring (8 of 19) received maternal antibodies. In experiments 2 and 3, MHV antibodies were detected neither in the recipients nor in the offspring. These results indicate, for the first time, that even if the zona pellucida is partially disrupted by laser microdissection, the transmission of MHV-A59 can be avoided by correctly performed washing steps in the IVF-ET procedure.

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Section: Serological Analysismentioning

confidence: 99%

Risk Assessment of Mouse Hepatitis Virus Infection via In Vitro Fertilization and Embryo Transfer by the Use of Zona-Intact and Laser-Microdissected Oocytes1

Peters

Marschall

Mahabir

et al. 2006

Biology of Reproduction

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“…Crl:CD1(Icr) mice are typically used as ET recipients at many facilities. With respect to the virus, we used the MMVp to extend the results of previous studies [18,26,28], thereby enabling comparison of data.…”

Section: Discussionmentioning

confidence: 99%

“…In total, sequential drops from 40 and 18 sets of IVF and culture dishes from the CDO and CEO groups, respectively, were analyzed. All drops were diluted 1:10 with DMEM and 10% FCS and were tested for the presence of MMVp by PCR and for virus infectivity by in vitro infectivity assay (IVIA) in cell culture using the protocols previously described [18,26]. DMEM containing 10% FCS was used as the diluent to facilitate cell culture.…”

Section: Virological Examination Of Washing Drops and Embryosmentioning

confidence: 99%

Lack of Transmission of Mouse Minute Virus (MMV) from In Vitro-Produced Embryos to Recipients and Pups Due to the Presence of Cumulus Cells During the In Vitro Fertilization Process

Mahabir¹,

Bulian²,

Needham³

et al. 2009

Biology of Reproduction

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The risk of transmission of mouse minute virus (MMV) to recipients of murine embryos arising from in vitro fertilization (IVF) of cumulus-enclosed oocytes (CEOs) or without cumulus cells (CDOs) in the presence of MMV-exposed (10(4) TCID(50) [mean tissue culture infective dose]/ml MMVp [prototype strain of MMV]) spermatozoa was evaluated. Also, the time after embryo transfer to detection of MMV antibody and the presence of MMV DNA in the mesenteric lymph nodes of recipients and pups were investigated. All mice were MMV free, but two seropositive recipients and four seropositive pups were found in the group with CDOs. With regard to the CEOs, two of 11 holding drops and five of 11 groups of embryos were MMV positive using PCR, while neither holding drops nor embryos carried infectious MMVp, as evidenced by the in vitro infectivity assay. From IVF with CDOs, five of 14 holding drops and four of nine groups of embryos were MMV positive, while one of 14 holding drops and no embryos carried infectious MMVp. When 10(5) cumulus cells were analyzed 5 h after exposure to 10(4) TCID(50)/ml MMVp, cells had an average titer of 10(4) TCID(50)/ml MMVp. The present data show that, in contrast to CDOs, 2-cell embryos from CEOs did not transmit infectious MMVp to the holding drops and to recipients. This observation is due to the presence of cumulus cells during the IVF process that reduce entry of MMV into the zona pellucida and absorb some of the virus. These data further confirm the efficacy of the IVF procedure in producing embryos that are free of infectious virus, leading to virus-free seronegative recipients and rederived pups.

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“…If it is not possible to separate the species, mice and rats may be grown in the same area due to their similar pathogenic profile and compatible behavior (National…, 2003). Nowadays, transference of biological material and genetically modified mice among different institutions, with no regular quarantine sanitary programs and caesarian rederivation of the animals, has also enabled dissemination of pathogenic agents among colonies (Nicklas and Weiss, 2000;Mahabir et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Sanitary profile in mice and rat colonies in laboratory animal houses in Minas Gerais: I - Endo and ectoparasites

Bicalho¹,

Araújo²,

Rocha³

et al. 2007

Arq. Bras. Med. Vet. Zootec.

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The sanitary conditions of 13 animal houses in nine public institutions in Minas Gerais, and the presence of endo and ectoparasites of mice and rats colonies kept in these facilities were evaluated. Data about barriers to prevent the transmission of diseases and a program of sanitary monitoring were obtained through a questionnaire and local visit. Parasitological methods were performed for diagnosing mite, lice, helminthes, and protozoa parasites in 344 mice and 111 rats. Data have shown that the majority of the animal houses had neither proper physical environment nor protection barriers to prevent the transmission of infections. Parasitological results have shown that only one animal house (7.7%) had parasite free animals, whereas the others have presented infected animals and the prevalences of parasites in the mice colonies were: Myobia musculi ( Palavras-chave: camundongo, rato, biotério, parasito, barreira sanitária

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Mouse antibody production test: can we do without it?

Cited by 14 publications

References 17 publications

Risk Assessment of Mouse Hepatitis Virus Infection via In Vitro Fertilization and Embryo Transfer by the Use of Zona-Intact and Laser-Microdissected Oocytes1

Risk Assessment of Mouse Hepatitis Virus Infection via In Vitro Fertilization and Embryo Transfer by the Use of Zona-Intact and Laser-Microdissected Oocytes1

Lack of Transmission of Mouse Minute Virus (MMV) from In Vitro-Produced Embryos to Recipients and Pups Due to the Presence of Cumulus Cells During the In Vitro Fertilization Process

Sanitary profile in mice and rat colonies in laboratory animal houses in Minas Gerais: I - Endo and ectoparasites

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