Mouse linkage cytogenetics (L-C) probes

Liechty, Melissa C.; Clase, A. C.; Puschus, K. L.; Aytay, S.; Carpio, C. M.; Hall, Brad; Hozier, John

doi:10.1159/000015511

Cited by 6 publications

(2 citation statements)

References 14 publications

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“…The problem, that mouse chromosomes are hard to characterize by GTGbanding and SKY was recognized and tackled previously; i.e. at least two groups developed some kind of YAC/BAC-based chromosome bar code for mouse chromosomes (21)(22)(23)(24). However, no further applications of these approaches were published subsequently.…”

Section: Resultsmentioning

confidence: 99%

Molecular cytogenetic characterization of the mouse cell line WMP2 by spectral karyotyping and multicolor banding applying murine probes

Karst

Трифонов²,

Romanenko³

et al. 2006

Int J Mol Med

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The Moloney murine leukemia virus-transformed suspension cell line WMP2 is derived from wild mice (Mus musculus) of the WMP/WMP strain. These mice carry nine pairs of metacentric Robertsonian translocation chromosomes. As the chromosomes of the wild-type mouse are all acrocentric, metaphase spreads of the WMP2 cells seam to be highly suited for physical gene mapping. Here we studied the WMP2 line using spectral karyotyping (SKY) combined with new established mouse specific multicolor banding (mcb) probes for the chromosomes X, 3, 4, 6 and 18. SKY revealed that the WMP2 cell line developed further four derivative chromosomes. After application of mcb five previously unrecognizable intrachromosomal rearrangements with 9 breakpoints were detected for the studied chromosomes.

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Section: Resultsmentioning

confidence: 99%

Molecular cytogenetic characterization of the mouse cell line WMP2 by spectral karyotyping and multicolor banding applying murine probes

Karst

Трифонов²,

Romanenko³

et al. 2006

Int J Mol Med

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“…As a second tactic, the same group published a set of 149 mouse linkage cytogenetics (L-C) probes at 10-cM intervals. This probe set was designed as a two-color FISH probe set (Liechty et al, 2000). Finally, Henegariu et al (2001) published a triplecolor FISH probe set for mouse chromosome identification based on subtelomeric and subcentromeric probes labeled in three different fluorochromes.…”

Section: Fish-banding Methods In Micementioning

confidence: 99%

Multicolor fluorescence in situ hybridization (FISH) applied to FISH-banding

Liehr

Starke

Heller

et al. 2006

Cytogenet Genome Res

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During the last decade not only multicolor fluorescence in situ hybridization (FISH) using whole chromosome paints as probes, but also numerous chromosome banding techniques based on FISH have been developed for the human and for the murine genome. This review focuses on such FISH-banding techniques, which were recently defined as ‘any kind of FISH technique, which provide the possibility to characterize simultaneously several chromosomal subregions smaller than a chromosome arm. FISH-banding methods fitting that definition may have quite different characteristics, but share the ability to produce a DNA-specific chromosomal banding’. While the standard chromosome banding techniques like GTG lead to a protein-related black and white banding pattern, FISH-banding techniques are DNA-specific, more colorful and, thus, more informative. For some, even high-resolution FISH-banding techniques the development is complete and they can be used for whole genome hybridizations in one step. Other FISH-banding methods are only available for selected chromosomes and/or are still under development. FISH-banding methods have successfully been applied in research in evolution- and radiation-biology, as well as in studies on the nuclear architecture. Moreover, their suitability for diagnostic purposes has been proven in prenatal, postnatal and tumor cytogenetics, indicating that they are an important tool with the potential to partly replace the conventional banding techniques in the future.

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