Mouse Models for Use in Cryptosporidium Infection Studies and Quantification of Parasite Burden Using Flow Cytometry, qPCR, and Histopathology

Sonzogni-Desautels, Karine; Mead, Jan R.; Ndao, Momar

doi:10.1007/978-1-4939-9748-0_14

Cited by 5 publications

(2 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast, the intensity and duration of oocyst shedding in mice infected with IIdA19G1-GD were just slightly higher than those in mice infected with IIa-Waterborne and were not associated with any clinical signs. Differences in infectivity and virulence are well known among IIa isolates [ 10 , 31 , 32 ]. In the present study, the two IId isolates with lower infective doses, higher and longer oocyst shedding, and higher virulence in GKO mice were from dairy farms with cryptosporidiosis outbreaks and the associated mortality, indicating that there is correlation in the virulence of C .…”

Section: Discussionmentioning

confidence: 99%

High infectivity and unique genomic sequence characteristics of Cryptosporidium parvum in China

Jia

Huang

et al. 2022

PLoS Negl Trop Dis

View full text Add to dashboard Cite

Zoonotic Cryptosporidium parvum infections are mainly caused by IIa and IId subtypes. As most biological characterizations have been performed on IIa subtypes, the biological and genetic characteristics of IId subtypes in China are not clear. We evaluated the infection and genetic characteristics of IId isolates in interferon-γ-knockout mice using qPCR to quantify oocyst shedding, histological examination to monitor pathological changes and comparative genomic analyses to identify infectivity and virulence-associated differences. Compared with the reference IIa isolate, mice infected with the IId isolates had significantly higher and longer oocyst shedding and lower body weight gain. In addition, the four IId isolates examined differed significantly in infectivity (as indicated by the half infective dose), oocyst shedding duration, and pathogenicity. Comparative genomic analysis indicated that the IId isolates had three more subtelomeric genes than the reference IIa isolate and 5385–5548 nucleotide substitutions, with the hypervariable genes mostly in two blocks on chromosome 1. In contrast, the four IId isolates differed from each other by 77–1,452 nucleotides, with virulence-associated sequence differences mainly in nine genes within a 28-kb block on chromosome 6. These data indicate the newly emerged C. parvum IId subtypes in China have high animal infectivity and unique genomic characteristics.

show abstract

Section: Discussionmentioning

confidence: 99%

High infectivity and unique genomic sequence characteristics of Cryptosporidium parvum in China

Jia

Huang

et al. 2022

PLoS Negl Trop Dis

View full text Add to dashboard Cite

show abstract

“…In addition, both IbA12G3 and InA17 infections resulted in significantly higher parasite loads in the mouse intestine than ImA20, with the former two also causing reductions in body weight gain, consistent with their much lower ID50 values. Previously, differences in infectivity and virulence have been observed between different subtype families of C. parvum and C. tyzzeri [29][30][31] , and between different isolates of the same gp60 subtype [32][33][34] . Results from comparative genomic analysis indicate that sequence differences in genes encoding mucin glycoproteins and other secretory proteins are associated with different phenotypic traits of isolates in mice 29,30 .…”

Section: Discussionmentioning

confidence: 99%

Unique genomic characteristics underlie the infectivity of divergent Cryptosporidium hominis subtypes to animals

Xiao,

Huang,

et al. 2023

Preprint

View full text Add to dashboard Cite

The anthroponotic Cryptosporidium hominis differs from the zoonotic C. parvum in its lack of infectivity to rodents, but several divergent C. hominis subtypes has been recently found in nonhuman primates and equines. Here, we performed whole-genome sequencing of 17 animal C. hominis isolates. In a comparative analysis with 222 human isolates, the animal-adapted isolates showed significant genetic divergence, and genetic recombination shaped their population. In addition, these animal isolates had additional genes found in C. parvum and significant sequence differences in subtelomeric genes. In interferon-γ knockout mice, three monkey isolates showed differences in infectivity and induced higher and longer oocyst shedding than a reference C. parvum isolate. Two of the isolates were fluorescently tagged, allowing the screening of the progeny of a genetic cross. These data provide insight into genetic determinants of host range and virulence in Cryptosporidium, and a convenient animal model for biological studies of C. hominis.

show abstract

A productive immunocompetent mouse model of cryptosporidiosis with long oocyst shedding duration for immunological studies

Huang

Sun

et al. 2022

Journal of Infection

View full text Add to dashboard Cite

Mouse Models for Use in Cryptosporidium Infection Studies and Quantification of Parasite Burden Using Flow Cytometry, qPCR, and Histopathology

Cited by 5 publications

References 29 publications

High infectivity and unique genomic sequence characteristics of Cryptosporidium parvum in China

High infectivity and unique genomic sequence characteristics of Cryptosporidium parvum in China

Unique genomic characteristics underlie the infectivity of divergent Cryptosporidium hominis subtypes to animals

A productive immunocompetent mouse model of cryptosporidiosis with long oocyst shedding duration for immunological studies

Contact Info

Product

Resources

About