Mouse monoclonal antibodies against rabbit b6 allotype and their use for characterization of light-chain subpopulations

Internal Images of Rabbit Immunoglobulin Allotopes

“…This finding has been true for all systems thus far studied including, in addition to the above example, the al (Roux & Metzger 1984) and b4 (Roland & Cazenave, unpublished) systems. Returning to the b6 system, we recently prepared monoclonal antibodies in the mouse which were directed against different allotopes of the b6 rabbit allotype (Petit-Koskas et al 1983). These antibodies all bound to rabbit Ab2 prepared against rabbit anti-b6 Abi.…”

“…Groups of BALB/c mice were immunized against different anti-b6 monoclonal antibodies. One of these antibodies was antibody 27, an antibody reactive with the b6 allotype and non-cross-reactive with the b4, b5 or b9 allotypes (Petit-Koskas et al 1983). Polyclonal anti-27 Ab2 was isolated and injected into a third series of BALB/c mice to raise Ab3 antibodies.…”