Mouse‐Protective Antibodies Against The Ibc Proteins of Group B Streptococci

Bevanger, Lars; Néss, Augusta Irene

doi:10.1111/j.1699-0463.1985.tb02862.x

Cited by 26 publications

(10 citation statements)

References 11 publications

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“…In 1975, Rebecca Lancefield first demonstrated that immunization of rabbits with C protein containing GBS strains elicited antibodies that provided passive protection against lethal challenge in mice with strains containing this protein [21]. Investigators later determined that the C protein was comprised of α and β components and found that rabbit antibodies to either component of C protein conferred passive protection in mice [22]. In the 1990's, a monoclonal antibody to α C protein was described that induced opsonic killing of GBS and protected mice from lethal challenge with GBS [23].…”

Section: Discussionmentioning

confidence: 99%

Alpha C protein-specific immunity in humans with group B streptococcal colonization and invasive disease

Pannaraj

Kelly

Rench

et al. 2008

Vaccine

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Alpha C protein, found in 76% of non-type III strains of group B Streptococcus (GBS), elicits antibodies protective against alpha C-expressing strains in experimental animals, making it an appealing carrier for a GBS conjugate vaccine. We determined whether natural exposure to alpha C elicits antibodies in women. Geometric mean concentrations of alpha C-specific IgM and IgG were similar by ELISA in sera from 58 alpha C GBS strain colonized and 174 age-matched non-colonized women (IgG 245 and 313 ng/ml; IgM 257 and 229 ng/ml, respectively), but acute sera from 13 women with invasive alpha C-expressing GBS infection had significantly higher concentrations (IgM 383 and IgG 476 ng/ml [p=0.036 and 0.038, respectively]). Convalescent sera from 5 of these women 16-49 days later had high alpha C-specific IgM and IgG concentrations (1355 and 4173 ng/ml, respectively). In vitro killing of alpha C-expressing GBS correlated with total alpha C-specific antibody concentration. Invasive disease but not colonization elicits alpha C-specific IgM and IgG in adults.

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Section: Discussionmentioning

confidence: 99%

Alpha C protein-specific immunity in humans with group B streptococcal colonization and invasive disease

Pannaraj

Kelly

Rench

et al. 2008

Vaccine

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“…In addition to the CPS, proteins are also present on the surfaces of GBS cells, and studies have shown that antibodies against both CPS and surface proteins are protective against GBS infection (67)(68)(69). The alpha-like protein (Alp) family proteins are among the well-characterized GBS surface proteins and are significant virulence factors.…”

Section: Surface Proteinsmentioning

confidence: 99%

Perinatal Streptococcus agalactiae Epidemiology and Surveillance Targets

2018

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SUMMARY, or group B streptococcus (GBS), is a major neonatal pathogen. Recent data have elucidated the global prevalence of maternal and neonatal colonization, but gaps still remain in the epidemiology of this species. A number of phenotypic and genotypic classifications can be used to identify the diversity of GBS strains, and some are more discriminatory than others. This review explores the main schemes used for GBS epidemiology and further details the targets for epidemiological surveillance. Current screening practices across the world provide a unique opportunity to gain detailed information on maternal colonizing strains and neonatal disease-causing strains, which is vital for monitoring and therapeutics, if sufficient detail can be extracted. Deciphering which isolates are circulating within specific populations and recording targets within invasive strains are crucial steps in monitoring the implementation of therapeutics, such as vaccines, as well as developing novel therapies against prevalent GBS strains. Having a detailed understanding of global GBS epidemiology will prove invaluable for understanding the pathogenesis of this organism and equipping future prevention strategies for success.

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“…However, in a mouse model, protection by antibodies to the Ibc protein antigens was demonstrated (14). Recent results in this laboratory have substantiated that antibodies to each of the a and p antigens are protective in an experimental model (8).…”

Section: Discussionmentioning

confidence: 95%

The Ibc Proteins Of Group b Streptococci: Isolation Of The α and β Antigens by Immunosorbent Chromatography and Test For Human Serum Antibodies Against The Two Antigens

Bevanger¹

1985

Acta Pathologica Microbiologica Scandinavica Series B: Microbio

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The Ibc protein fraction from group B streptococci contains at least two antigens, named α and β. Rabbit antisera to each of these antigens were used to prepare affinity‐purified α and β antigens. Analysis of the purified antigens by SDS‐PAGE showed at least three weakly stained proteins in the α antigen ‐and multiple protein bands in the β antigen preparation. Conditions for optimalization of ELISA for testing of human serum antibodies against these antigens were investigated. When tested at a dilution of 1 in 200, ELISA activity corresponding to an OD405 > 0.2 was shown by 11.2 per cent of 157 blood donor sera in tests with the α antigen, and 77.7 per cent with the β antigen. Increasing antibody activity against the α antigen was shown by a patient with septicaemia caused by an α antigen‐producing strain. The antigens were immunoblotted and tested against human and rabbit antibodies. With the α antigen, antibodies from both species produced a diffuse stained area, and with the β antigen multiple stained bands, some of which were colinear with the human and rabbit antibody. Thus, antibodies to the β antigen of group B streptococci are present in a large proportion of the healthy population. These antibodies may be important in the protection against group B streptococcal disease.

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Mouse‐Protective Antibodies Against The Ibc Proteins of Group B Streptococci

Cited by 26 publications

References 11 publications

Alpha C protein-specific immunity in humans with group B streptococcal colonization and invasive disease

Alpha C protein-specific immunity in humans with group B streptococcal colonization and invasive disease

Perinatal Streptococcus agalactiae Epidemiology and Surveillance Targets

The Ibc Proteins Of Group b Streptococci: Isolation Of The α and β Antigens by Immunosorbent Chromatography and Test For Human Serum Antibodies Against The Two Antigens

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