Mouse RanBPM is a partner gene to a germline specific RNA helicase, mouse vasa homolog protein

Shibata, Norito; Tsunekawa, Naoki; Okamoto-Ito, Shino; Akasu, Ryuko; Tokumasu, Ako; Noce, Toshiaki

doi:10.1002/mrd.20009

Cited by 36 publications

(25 citation statements)

References 36 publications

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“…Among these were a SNc/Tudor domain protein (spot 3), a polyadenylation-binding protein (PABP; spot 10), and several RNA helicases (spots 10,15,22,23,30,32,37). Homologs of these proteins have been shown to associate with MTs, either directly or indirectly.…”

Section: Resultsmentioning

confidence: 99%

“…Homologs of these proteins have been shown to associate with MTs, either directly or indirectly. For example, PABP co-purified with MTs from sea urchin embryos and HeLa cells (30,31), and a human RNA helicase (Vasa) interacts with a MT-binding protein that regulates MT nucleation (32). A protein similar to the SNc/Tudor domaincontaining protein has also been identified in cytoskeleton fractions from rice and pea (33,34).…”

Section: Resultsmentioning

confidence: 99%

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Large-scale Identification of Tubulin-binding Proteins Provides Insight on Subcellular Trafficking, Metabolic Channeling, and Signaling in Plant Cells

Chuong

Good

Taylor

et al. 2004

Molecular & Cellular Proteomics

115

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Microtubules play an essential role in the growth and development of plants and are known to be involved in regulating many cellular processes ranging from translation to signaling. In this article, we describe the proteomic characterization of Arabidopsis tubulin-binding proteins that were purified using tubulin affinity chromatography. Microtubule co-sedimentation assays indicated that most, if not all, of the proteins in the tubulin-binding protein fraction possessed microtubule-binding activity. Two-dimensional gel electrophoresis of the tubulin-binding protein fraction was performed, and 86 protein spots were excised and analyzed for protein identification. A total of 122 proteins were identified with high confidence using LC-MS/MS. These proteins were grouped into six categories based on their predicted functions: microtubule-associated proteins, translation factors, RNA-binding proteins, signaling proteins, metabolic enzymes, and proteins with other functions. Almost one-half of the proteins identified in this fraction were related to proteins that have previously been reported to interact with microtubules. This study represents the first large-scale proteomic identification of eukaryotic cytoskeleton-binding proteins, and provides insight on subcellular trafficking, metabolic channeling, and signaling in plant cells. Molecular & Cellular Proteomics 3:970 -983, 2004.The cytoskeleton is the single most important structure that contributes to the highly ordered organization of the eukaryotic cell. It provides a framework for cell division and the trafficking of organelles and macromolecules, and also serves to regulate important cellular processes such as signaling, translation, and metabolism. The cytoskeleton plays a key role in a number of plant-specific processes, such as assisting in the formation of the cell plate, regulating cell-to-cell movement, and influencing the direction of cell elongation (1). A role for the microtubule (MT) 1 component of the cytoskeleton in many of these processes has been demonstrated, and a number of MT-binding proteins that are responsible for regulating these events have been identified.Plant MTs are assembled into four distinct arrays during the cell cycle (2). Three of these arrays-the interphase cortical array, the pre-prophase band, and the phragmoplast-have no counterpart in animal cells. The cortical MT array has been linked to the regulation of cellulose microfibril deposition and, hence, a role in cell expansion, while the pre-prophase band and the phragmoplast have important roles in the positioning and synthesis of the new cell plate in dividing cells. The fourth array, the spindle, has an evolutionarily conserved role in the segregation of chromosomes during cell division. The organization and dynamics of MTs in these arrays depend on the activity of various MT-associated proteins (MAPs). Several plant MAPs have been identified, including the 65-kDa MAPs, MAP 190, and MOR1 (3). These proteins are important in cross-bridging MTs, linking MTs with actin filamen...

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Large-scale Identification of Tubulin-binding Proteins Provides Insight on Subcellular Trafficking, Metabolic Channeling, and Signaling in Plant Cells

Chuong

Good

Taylor

et al. 2004

Molecular & Cellular Proteomics

115

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“…For example, the mouse vasa homolog gene (Mvh; Ddx4 -Mouse Genome Informatics), encoding an ATPdependent RNA helicase, is crucial for the development of male germ cells (Shibata et al, 2004;Tanaka et al, 2000). However, in Mvh-null males, germ cell differentiation stops earlier than in RanBPM -/-mice, at the zygotene stage of prophase I, and Mvh -/-females are fertile.…”

Section: Discussionmentioning

confidence: 99%

RanBPM is essential for mouse spermatogenesis and oogenesis

et al. 2011

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SUMMARYRanBPM is a recently identified scaffold protein that links and modulates interactions between cell surface receptors and their intracellular signaling pathways. RanBPM has been shown to interact with a variety of functionally unrelated proteins; however, its function remains unclear. Here, we show that RanBPM is essential for normal gonad development as both male and female RanBPM -/-mice are sterile. In the mutant testis there was a marked decrease in spermatogonia proliferation during postnatal development. Strikingly, the first wave of spermatogenesis was totally compromised, as seminiferous tubules of homozygous mutant animals were devoid of post-meiotic germ cells. We determined that spermatogenesis was arrested around the late pachytene-diplotene stages of prophase I; surprisingly, without any obvious defect in chromosome synapsis. Interestingly, RanBPM deletion led to a remarkably quick disappearance of all germ cell types at around one month of age, suggesting that spermatogonia stem cells are also affected by the mutation. Moreover, in chimeric mice generated with RanBPM -/-embryonic stem cells all mutant germ cells disappeared by 3 weeks of age suggesting that RanBPM is acting in a cell-autonomous way in germ cells. RanBPM homozygous mutant females displayed a premature ovarian failure due to a depletion of the germ cell pool at the end of prophase I, as in males. Taken together, our results highlight a crucial role for RanBPM in mammalian gametogenesis in both genders.

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“…Vasa/MVH, a germ cell-specific RNA helicase, interacts with RanBPM, which recruits RanGTP. The resulting complex may play a role in trapping and transferring of specific RNAs from the nucleus to the chromatoid body (21). However it was noted that the binding of DDX3 to CRM1 did not required its N-terminal NES (rather a C-terminal sequences between aa 260 and 517) and RanGTP (22) for HIV-1 Rev-PRE export.…”

Section: Discussionmentioning

confidence: 99%

Gonadotropin-regulated Testicular RNA Helicase (GRTH/Ddx25) Is a Transport Protein Involved in Gene-specific mRNA Export and Protein Translation during Spermatogenesis

Yi¹,

Tsai‐Morris²,

Gutti

et al. 2006

Journal of Biological Chemistry

105

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Gonadotropin-regulated testicular RNA helicase (GRTH/ Ddx25), a member of the DEAD-box protein family, is essential for completion of spermatogenesis. GRTH is present in the cytoplasm and nucleus of meiotic spermatocytes and round spermatids and functions as a component of mRNP particles, implicating its post-transcriptional regulatory roles in germ cells. In this study, GRTH antibodies specific to N-or C-terminal sequences showed differential subcellular expression of GRTH 56-and 61-kDa species in nucleus and cytoplasm, respectively, of rodent testis and transfected COS1 cells. The 56-kDa nuclear species interacted with CRM1 and participated in mRNA transport. The phosphorylated cytoplasmic 61-kDa species was associated with polyribosomes. Confocal studies on COS-1 cells showed that GRTH-GFP was retained in the nucleus by treatment with a RNA polymerase inhibitor or the nuclear protein export inhibitor. This indicated that GRTH is a shuttling protein associated with RNA export. The N-terminal leucine-rich region (61-74 amino acids) was identified as the nuclear export signal that participated in CRM1-dependent nuclear export pathway. Deletion analysis identified a 14-amino acid GRTH sequence (100 -114 amino acids) as a nuclear localization signal. GRTH selectively regulated the translation of specific genes including histone 4 and HMG2 in germ cells. In addition, GRTH participated in the nuclear export of RNA messages (PGK2, tACE, and TP2) in a gene-specific manner. These studies strongly indicate that the mammalian GRTH/Ddx25 gene is a multifunctional RNA helicase that is an essential regulator of sperm maturation.Modulation of RNA structure by members of the DEAD-box family of RNA helicases is a crucial step in many fundamental biological processes (1-7). This class of proteins participates in several aspects of RNA metabolism and translational events, including pre-mRNA splicing, ribosome biogenesis, nucleo-cytoplasmic transport, translation, and RNA decay that ultimately regulate organelle gene expression for specific biological functions. RNA helicases contain at least eight conserved motifs, some of which have recognized functions such as ATP hydrolysis, RNA binding, and RNA unwinding that modulate RNA activity, while their N-or C-terminal extensions may confer substrate specificity.Gene expression in germ cells requires temporal uncoupling of transcription and translation (8, 9). Two-thirds of the mRNAs in the adult mammalian testis are associated with specific proteins to form messenger ribonuclear protein (mRNP) 3 particles, and are stored in the cytoplasm of spermatids for translation at specific times required for progression and completion of spermatogenesis. We have recently discovered and characterized a hormone-dependent testis-specific RNA helicase in Leydig and germinal cells (meiotic spermatocytes and spermatids) of rat, mouse, and human, termed gonadotropinregulated testicular helicase (GRTH/Ddx25) (10). This enzyme is present in the nucleus and cytoplasm of germ cells, and selectively binds ...

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Mouse RanBPM is a partner gene to a germline specific RNA helicase, mouse vasa homolog protein

Cited by 36 publications

References 36 publications

Large-scale Identification of Tubulin-binding Proteins Provides Insight on Subcellular Trafficking, Metabolic Channeling, and Signaling in Plant Cells

Large-scale Identification of Tubulin-binding Proteins Provides Insight on Subcellular Trafficking, Metabolic Channeling, and Signaling in Plant Cells

RanBPM is essential for mouse spermatogenesis and oogenesis

Gonadotropin-regulated Testicular RNA Helicase (GRTH/Ddx25) Is a Transport Protein Involved in Gene-specific mRNA Export and Protein Translation during Spermatogenesis

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