Mouse T cell antigen receptor: Structure and organization of constant and joining gene segments encoding the β polypeptide

Malissen, Marie; Minard, Karyl I.; Mjolsness, Shelley; Kronenberg, Mitchell; Goverman, Joan; Hunkapiller, Tim; Prystowsky, Michael B.; Yoshikai, Yasunobu; Fitch, Frank W.; Mak, Tak W.; Hood, Leroy

doi:10.1016/0092-8674(84)90444-6

Cited by 400 publications

(189 citation statements)

References 27 publications

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“…There are multiple base changes and several amino acid changes between the two constant region genes, Co31 and C(2, in both mouse and human, and the 3'-untranslated regions are 50% divergent Malissen et al, 1984;Yoshikai et al, 1984b). The coding and 3'-untranslated sequences shown here clearly correspond to the previously published C(3 sequence from Jurkat ascribed to C(31 (Sims et al, 1984).…”

Section: Discussionmentioning

confidence: 54%

“…cDNA clones which correspond to the /3-chain mRNA have recently been isolated (Hedrick et al, 1984a(Hedrick et al, , 1984bYanagi et al, 1984). The predicted amino acid sequence has homology with immunoglobulin heavy and light chains and characterisation of corresponding genomic clones has demonstrated the presence of variable, diversity, joining and constant gene segments, further supporting the relationship with immunoglobulins Gascoigne et al, 1984;Malissen et al, 1984;Siu et al, 1984aSiu et al, , 1984b. Analysis of genomic clones hybridising with a constant region probe has localised the mouse /-chain genes into two clusters ofjoining regions and two constant regions in the order JT C,B1, JT C/32 .…”

Section: Introductionmentioning

confidence: 82%

“…Recognition sequences for V-D and D-J recombination follow rules first established for immunoglobulin (Early et al, 1980). The recognition sequences in the T-cell receptor (-chain locus include a highly conserved heptamer with the bases TGTG situated immediately 5' to every joining region gene in both human (Siu et al, 1984a) and mouse Malissen et al, 1984). Inspection of the sequence shown in Figure 2 reveals that no such sequence exists immediately 5' to the boundary of the joining region.…”

Section: Discussionmentioning

confidence: 99%

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Transcripts from an aberrantly re-arranged human T-cell receptor beta-chain gene.

Collins¹,

Kissonerghis²,

Dunne³

et al. 1985

The EMBO Journal

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A number of cDNA clones homologous to the human T-celi receptor ,3-chain gene have been isolated from a library constructed from the human leukaemic cell line Jurkat. The nucleotide sequences of two of these clones demonstrate thatJurkat synthesises an RNA, probably derived from an aberrantly rearranged CO1 gene, which cannot encode a fnctional /-chain. This transcript contains a novel joining region and similar transcripts appear to be relatively abundant in several T-celi lines.

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Section: Discussionmentioning

confidence: 54%

Section: Introductionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Transcripts from an aberrantly re-arranged human T-cell receptor beta-chain gene.

Collins¹,

Kissonerghis²,

Dunne³

et al. 1985

The EMBO Journal

View full text Add to dashboard Cite

show abstract

“…The discovery that the immense diversity of the T cell receptor (TCR) repertoire is based on chromosomal rearrangements [26][27][28] once more ignited the hunt for factors and forces that were driving negative selection. A logical consequence of the random nature of TCR rearrangement is that auto-reactive clones will be generated and must therefore be eliminated during thymocyte maturation to preserve tolerance.…”

Section: Apoptosis In the Immune System Central Tolerance -Education mentioning

confidence: 99%

How apoptosis got the immune system in shape

Feig

Marynen

2007

Eur. J. Immunol.

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“…Briefly, for TCR-b rearrangement analysis, 10 mg of DNA from thymus, spleen and liver was digested with either HindIII or PvuII, transferred onto a Hybond-N membrane (Amersham) and hybridized with a-32 P-labeled probe. The HindIII filter was hybridized with a J-b2 probe (Malissen et al, 1984) to obtain a spectrum of TCR-b gene rearrangement, and the obtained rearrangements were further confirmed by hybridizing the PvuII filter with a J-b1 probe (Malissen et al, 1984). For IgH rearrangement analysis, genomic DNA isolated from spleen, liver and lymph node was digested with BglII and hybridized with a-32 P-labeled J H probe (Sakano et al, 1980).…”

Section: Tcr and Igh Rearrangement Analysismentioning

confidence: 99%

Overexpression of a transcription factor LYL1 induces T- and B-cell lymphoma in mice

et al. 2007

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LYL1, a member of the class II basic helix-loop-helix transcription factors, is aberrantly expressed in a fraction of human T-cell acute lymphoblastic leukemia. Here, we generated transgenic mice ubiquitously overexpressing LYL1 using a construct expressing full-length cDNA driven by a human elongation factor 1a promoter. Four independent lines exhibiting high LYL1 expression were established. Of these transgenic mice, 96% displayed loss of hair with a short kinked tail. Furthermore, 30% of them developed malignant lymphoma, with an average latent period of 352 days. In these mice, histological examination revealed tumor cell infiltration in multiple organs and immunohistochemical analysis showed that the infiltrated tumor cells were either CD3 or CD45R/B220-positive; fluorescence-activated cell sorter analysis indicated that each tumor consisted either of mainly CD4, CD8 double-positive T cells or mature B cells; the clonality of LYL1-induced lymphoma was confirmed by T-cell receptor rearrangement and immunoglobulin heavychain gene rearrangement analyses. Mammalian twohybrid analysis and luciferase assay suggested that excess LYL1 blocked the dimerization of E2A and thus inhibited the regulatory activity of E2A on the CD4 promoter. Reverse transcription-polymerase chain reaction results showed that the expression of certain E2A/HEB target genes was downregulated. Taken together, our results provide direct evidence that aberrant expression of LYL1 plays a role in lymphomagenesis.

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Mouse T cell antigen receptor: Structure and organization of constant and joining gene segments encoding the β polypeptide

Cited by 400 publications

References 27 publications

Transcripts from an aberrantly re-arranged human T-cell receptor beta-chain gene.

Transcripts from an aberrantly re-arranged human T-cell receptor beta-chain gene.

How apoptosis got the immune system in shape

Overexpression of a transcription factor LYL1 induces T- and B-cell lymphoma in mice

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