Mp52-08 3d Bioprinted Renal Tissue Constructs Using a Novel Kidney Ecm-Derived Bioink

Biomedical Application of Decellularized Scaffolds

“…258−260 Kidney disease has become an international public health problem. 261,262 Currently, the main treatments for end-stage renal disease are dialysis and kidney transplantation. But both methods have serious flaws.…”

“…In addition, factors such as diabetes, hypertension, hyperlipidemia, and smoking easily lead to chronic kidney disease. This has led to an increasing number of people suffering from chronic kidney disease. − Kidney disease has become an international public health problem. , Currently, the main treatments for end-stage renal disease are dialysis and kidney transplantation. But both methods have serious flaws.…”

Whole Organ Engineering: Approaches, Challenges, and Future Directions

“…Additive manufacturing methods for kidney engineering have been utilized as well, and are showing promise in being able to replicate the internal architecture of the kidney [112][113][114][115]. Ali et al published results of a novel ECM-derived, photo-crosslinkable bioink they had developed [112].…”

“…Constructs were printed using the kidney-derived bioink with human kidney cells included, with the resulting printed constructs retaining structural properties of native kidney tissue, and the cells remaining viable over time and maturing [112]. More recently, Carreno-Galeano et al utilized the same kidney-derived bioink to print ECM constructs laden with human kidney cells and implanted them in nude rats [115]. The constructs remained viable for two months, with comparisons of the implant site at the two-month timepoint to earlier times revealing that new tubular structures formed in the tissue around the implant, with human kidney cell markers detected in these new structures [115].…”

“…More recently, Carreno-Galeano et al utilized the same kidney-derived bioink to print ECM constructs laden with human kidney cells and implanted them in nude rats [115]. The constructs remained viable for two months, with comparisons of the implant site at the two-month timepoint to earlier times revealing that new tubular structures formed in the tissue around the implant, with human kidney cell markers detected in these new structures [115]. Host renal progenitor cell recruitment was observed in tissue surrounding acellular constructs that were implanted as well [115].…”